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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissimum L.) seed catalyzing the selective production of trilinolenin.
Pan X, Siloto RM, Wickramarathna AD, Mietkiewska E, Weselake RJ. Pan X, et al. Among authors: siloto rm. J Biol Chem. 2013 Aug 16;288(33):24173-88. doi: 10.1074/jbc.M113.475699. Epub 2013 Jul 2. J Biol Chem. 2013. PMID: 23824186 Free PMC article.
Antisense suppression of type 1 diacylglycerol acyltransferase adversely affects plant development in Brassica napus.
Lock YY, Snyder CL, Zhu W, Siloto RM, Weselake RJ, Shah S. Lock YY, et al. Among authors: siloto rm. Physiol Plant. 2009 Sep;137(1):61-71. doi: 10.1111/j.1399-3054.2009.01258.x. Epub 2009 Jun 1. Physiol Plant. 2009. PMID: 19602173
Directed evolution of acyl-CoA:diacylglycerol acyltransferase: development and characterization of Brassica napus DGAT1 mutagenized libraries.
Siloto RM, Truksa M, Brownfield D, Good AG, Weselake RJ. Siloto RM, et al. Plant Physiol Biochem. 2009 Jun;47(6):456-61. doi: 10.1016/j.plaphy.2008.12.019. Epub 2009 Jan 9. Plant Physiol Biochem. 2009. PMID: 19195902
High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function.
Chen G, Xu Y, Siloto RMP, Caldo KMP, Vanhercke T, Tahchy AE, Niesner N, Chen Y, Mietkiewska E, Weselake RJ. Chen G, et al. Among authors: siloto rmp. Plant J. 2017 Oct;92(2):167-177. doi: 10.1111/tpj.13652. Epub 2017 Sep 10. Plant J. 2017. PMID: 28755522 Free article.
Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds.
Wickramarathna AD, Siloto RM, Mietkiewska E, Singer SD, Pan X, Weselake RJ. Wickramarathna AD, et al. Among authors: siloto rm. BMC Biotechnol. 2015 Jun 30;15:63. doi: 10.1186/s12896-015-0156-6. BMC Biotechnol. 2015. PMID: 26123542 Free PMC article.
Acyl-CoA:diacylglycerol acyltransferase: molecular biology, biochemistry and biotechnology.
Liu Q, Siloto RM, Lehner R, Stone SJ, Weselake RJ. Liu Q, et al. Among authors: siloto rm. Prog Lipid Res. 2012 Oct;51(4):350-77. doi: 10.1016/j.plipres.2012.06.001. Epub 2012 Jun 15. Prog Lipid Res. 2012. PMID: 22705711 Review.
Functional and topological analysis of yeast acyl-CoA:diacylglycerol acyltransferase 2, an endoplasmic reticulum enzyme essential for triacylglycerol biosynthesis.
Liu Q, Siloto RM, Snyder CL, Weselake RJ. Liu Q, et al. Among authors: siloto rm. J Biol Chem. 2011 Apr 15;286(15):13115-26. doi: 10.1074/jbc.M110.204412. Epub 2011 Feb 14. J Biol Chem. 2011. PMID: 21321129 Free PMC article.
Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in a pah1Δ mutant strain of Saccharomyces cerevisiae.
Mietkiewska E, Siloto RM, Dewald J, Shah S, Brindley DN, Weselake RJ. Mietkiewska E, et al. Among authors: siloto rm. FEBS J. 2011 Mar;278(5):764-75. doi: 10.1111/j.1742-4658.2010.07995.x. Epub 2011 Jan 12. FEBS J. 2011. PMID: 21205207 Free article.
Role of cysteine residues in thiol modification of acyl-CoA:diacylglycerol acyltransferase 2 from yeast.
Liu Q, Siloto RM, Weselake RJ. Liu Q, et al. Among authors: siloto rm. Biochemistry. 2010 Apr 20;49(15):3237-45. doi: 10.1021/bi9020499. Biochemistry. 2010. PMID: 20225889
Simple methods to detect triacylglycerol biosynthesis in a yeast-based recombinant system.
Siloto RM, Truksa M, He X, McKeon T, Weselake RJ. Siloto RM, et al. Lipids. 2009 Oct;44(10):963-73. doi: 10.1007/s11745-009-3336-0. Epub 2009 Sep 10. Lipids. 2009. PMID: 19763656
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