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Binding proteins from alternative scaffolds.
Nygren PA, Skerra A. Nygren PA, et al. J Immunol Methods. 2004 Jul;290(1-2):3-28. doi: 10.1016/j.jim.2004.04.006. J Immunol Methods. 2004. PMID: 15261569 Review.
The quickly growing number of approaches can be classified into three groups: carrier proteins for the display of single variegated loops, scaffolds providing rigid elements of secondary structure, and protein frameworks supporting a group of conformationally variable loop …
The quickly growing number of approaches can be classified into three groups: carrier proteins for the display of single variegated loops, s …
Use of an albumin-binding domain for the selective immobilisation of recombinant capture antibody fragments on ELISA plates.
König T, Skerra A. König T, et al. J Immunol Methods. 1998 Sep 1;218(1-2):73-83. doi: 10.1016/s0022-1759(98)00112-4. J Immunol Methods. 1998. PMID: 9819124
A small albumin-binding domain (ABD) of 46 amino acids derived from streptococcal protein G was employed for the directed attachment of recombinant immunoglobulin (Ig) fragments to microtitre plates that had been coated with human serum albumin (HSA). ...Using the anti-lys
A small albumin-binding domain (ABD) of 46 amino acids derived from streptococcal protein G was employed for the directed attachment
Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold.
Beste G, Schmidt FS, Stibora T, Skerra A. Beste G, et al. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1898-903. doi: 10.1073/pnas.96.5.1898. Proc Natl Acad Sci U S A. 1999. PMID: 10051566 Free PMC article.
Hence, a lipocalin can be used for generating molecular pockets with a diversity of shapes. We term this class of engineered proteins "anticalins." Their one-domain scaffold makes them a promising alternative to antibodies to create a stable receptor p …
Hence, a lipocalin can be used for generating molecular pockets with a diversity of shapes. We term this class of engineered p …
Engineered protein scaffolds for molecular recognition.
Skerra A. Skerra A. J Mol Recognit. 2000 Jul-Aug;13(4):167-87. doi: 10.1002/1099-1352(200007/08)13:4<167::AID-JMR502>3.0.CO;2-9. J Mol Recognit. 2000. PMID: 10931555 Review.
This development started with the notion that immunoglobulins owe their function to the composition of a conserved framework region and a spatially well-defined antigen-binding site made of peptide segments that are hypervariable both in sequence and in conformation …
This development started with the notion that immunoglobulins owe their function to the composition of a conserved framework region a …
'Anticalins': a new class of engineered ligand-binding proteins with antibody-like properties.
Skerra A. Skerra A. J Biotechnol. 2001 Jun;74(4):257-75. doi: 10.1016/s1389-0352(01)00020-4. J Biotechnol. 2001. PMID: 11526907 Review.
The bilin-binding protein (BBP) was employed as a model system for the preparation of a random library with 16 selectively mutagenized residues. ...Compared with them, they exhibit however several advantages, including a smaller size, composition of a
The bilin-binding protein (BBP) was employed as a model system for the preparation of a random library with 16 selectively mut …
Duocalins: engineered ligand-binding proteins with dual specificity derived from the lipocalin fold.
Schlehuber S, Skerra A. Schlehuber S, et al. Biol Chem. 2001 Sep;382(9):1335-42. doi: 10.1515/BC.2001.166. Biol Chem. 2001. PMID: 11688717
Anticalins comprise a novel class of receptor proteins with predetermined ligand specificities which were engineered using the lipocalin fold. ...Both engineered lipocalins were previously selected from a random library prepared on the basis of the bilin-binding pro …
Anticalins comprise a novel class of receptor proteins with predetermined ligand specificities which were engineered using the lipoca …
Lipocalins in drug discovery: from natural ligand-binding proteins to "anticalins".
Schlehuber S, Skerra A. Schlehuber S, et al. Drug Discov Today. 2005 Jan 1;10(1):23-33. doi: 10.1016/S1359-6446(04)03294-5. Drug Discov Today. 2005. PMID: 15676296 Review.
Some lipocalins have a pathophysiological role, which opens possibilities for their use in medical applications. Furthermore, lipocalins from blood-sucking insects have evolved as scavengers for mediators of inflammation. ...These novel proteins have potential applications …
Some lipocalins have a pathophysiological role, which opens possibilities for their use in medical applications. Furthermore, lipocal …
Anticalins as an alternative to antibody technology.
Schlehuber S, Skerra A. Schlehuber S, et al. Expert Opin Biol Ther. 2005 Nov;5(11):1453-62. doi: 10.1517/14712598.5.11.1453. Expert Opin Biol Ther. 2005. PMID: 16255649 Review.
Anticalins are a class of engineered ligand-binding proteins that are based on the lipocalin scaffold. The lipocalin protein architecture is characterised by a compact, rigid beta-barrel that supports four structurally hypervariable loops. These loops form a
Anticalins are a class of engineered ligand-binding proteins that are based on the lipocalin scaffold. The lipocalin protein architec …
Alternative non-antibody scaffolds for molecular recognition.
Skerra A. Skerra A. Curr Opin Biotechnol. 2007 Aug;18(4):295-304. doi: 10.1016/j.copbio.2007.04.010. Epub 2007 Jul 20. Curr Opin Biotechnol. 2007. PMID: 17643280 Review.
Such engineered protein scaffolds are usually obtained by designing a random library with mutagenesis focused at a loop region or at an otherwise permissible surface area and by selection of variants against a given target via phage display or related techniq …
Such engineered protein scaffolds are usually obtained by designing a random library with mutagenesis focused at a loop region …
Anticalins as alternative binding proteins for therapeutic use.
Skerra A. Skerra A. Curr Opin Mol Ther. 2007 Aug;9(4):336-44. Curr Opin Mol Ther. 2007. PMID: 17694446 Review.
Members of the human lipocalin protein family exhibit four structurally hypervariable loops that form a ligand pocket, similar to the six complementarity-determining regions of antibodies. ...Compared with antibodies, anticalins provide several practical advantages because …
Members of the human lipocalin protein family exhibit four structurally hypervariable loops that form a ligand pocket, similar to the …
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