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Limited proteolysis unmasks specific DNA-binding of the murine RNA polymerase I-specific transcription termination factor TTFI.
Smid A, Finsterer M, Grummt I. Smid A, et al. J Mol Biol. 1992 Oct 5;227(3):635-47. doi: 10.1016/0022-2836(92)90213-4. J Mol Biol. 1992. PMID: 1404380
We have used limited proteolysis to purify and functionally characterize a TTFI core polypeptide (p50) which still specifically binds to the Sal-box target sequence and directs rDNA transcription termination. ...Additionally, we demonstrate that TTFI binds to DNA as a
We have used limited proteolysis to purify and functionally characterize a TTFI core polypeptide (p50) which still specifically binds …
Different domains of the murine RNA polymerase I-specific termination factor mTTF-I serve distinct functions in transcription termination.
Evers R, Smid A, Rudloff U, Lottspeich F, Grummt I. Evers R, et al. Among authors: smid a. EMBO J. 1995 Mar 15;14(6):1248-56. EMBO J. 1995. PMID: 7720715 Free PMC article.
Recombinant mTTF-I binds specifically to the murine terminator elements and terminates Pol I transcription in a reconstituted in vitro system. Deletion analysis has defined a modular structure of mTTF-I comprising a dispensable N-terminal half, a large …
Recombinant mTTF-I binds specifically to the murine terminator elements and terminates Pol I transcription in a reconstituted in vitr …
An undecamer DNA sequence directs termination of human ribosomal gene transcription.
Pfleiderer C, Smid A, Bartsch I, Grummt I. Pfleiderer C, et al. Among authors: smid a. Nucleic Acids Res. 1990 Aug 25;18(16):4727-36. doi: 10.1093/nar/18.16.4727. Nucleic Acids Res. 1990. PMID: 2395639 Free PMC article.
Previously we have shown that a repetitive 18 bp sequence motif, the Sal box (AGGTCGACCAGA/TT/ANTCCG), present in the 3' terminal spacer of mouse rDNA constitutes a termination signal for RNA polymerase I (pol I). ...A similar sequence motif with a 10 …
Previously we have shown that a repetitive 18 bp sequence motif, the Sal box (AGGTCGACCAGA/TT/ANTCCG), present in the 3' terminal spa …
A shared subunit belongs to the eukaryotic core RNA polymerase.
Lanzendörfer M, Smid A, Klinger C, Schultz P, Sentenac A, Carles C, Riva M. Lanzendörfer M, et al. Among authors: smid a. Genes Dev. 1997 Apr 15;11(8):1037-47. doi: 10.1101/gad.11.8.1037. Genes Dev. 1997. PMID: 9136931
The yeast RNA polymerase I is a multimeric complex composed of 14 distinct subunits, 5 of which are shared by the three forms of nuclear RNA polymerase. ...Electron microscopic visualization and electrophoretic analysis of the subunit depleted and reactivated forms of the …
The yeast RNA polymerase I is a multimeric complex composed of 14 distinct subunits, 5 of which are shared by the three forms of nucl …
The association of three subunits with yeast RNA polymerase is stabilized by A14.
Smid A, Riva M, Bouet F, Sentenac A, Carles C. Smid A, et al. J Biol Chem. 1995 Jun 2;270(22):13534-40. doi: 10.1074/jbc.270.22.13534. J Biol Chem. 1995. PMID: 7768955
Based on the amino acid sequence of one of the resulting peptides, a degenerate oligonucleotide was synthesized and used to isolate the RPA14 gene from a yeast subgenomic DNA library. ...This form of the enzyme was not active in a nonspecific in vitro transcr …
Based on the amino acid sequence of one of the resulting peptides, a degenerate oligonucleotide was synthesized and used to isolate t …
Polymorphic variation in TPMT is the principal determinant of TPMT phenotype: A meta-analysis of three genome-wide association studies.
Tamm R, Mägi R, Tremmel R, Winter S, Mihailov E, Smid A, Möricke A, Klein K, Schrappe M, Stanulla M, Houlston R, Weinshilboum R, Mlinarič Raščan I, Metspalu A, Milani L, Schwab M, Schaeffeler E. Tamm R, et al. Among authors: smid a. Clin Pharmacol Ther. 2017 May;101(5):684-695. doi: 10.1002/cpt.540. Epub 2017 Feb 1. Clin Pharmacol Ther. 2017. PMID: 27770449 Free PMC article. Review.
To examine this possibility, we performed a genome-wide association study (GWAS) of red blood cell TPMT activity in 844 Estonian individuals and 245 pediatric ALL cases. ...Only genetic variants mapping to chromosome 6, including the TPMT gene region, were significantly as …
To examine this possibility, we performed a genome-wide association study (GWAS) of red blood cell TPMT activity in 844 Estonian indi …
Lysosomal proteases cathepsins D, B, H, L and their inhibitors stefins A and B in head and neck cancer.
Kos J, Smid A, Krasovec M, Svetic B, Lenarcic B, Vrhovec I, Skrk J, Turk V. Kos J, et al. Among authors: smid a. Biol Chem Hoppe Seyler. 1995 Jul;376(7):401-5. doi: 10.1515/bchm3.1995.376.7.401. Biol Chem Hoppe Seyler. 1995. PMID: 7576236
Additionally, stefin A and stefin B were measured in tumour and normal tissue samples. In contrast to the cathepsins, the concentrations of these inhibitors of cysteine proteinases was not significantly different between tumour and normal samples. The concentrations of cat …
Additionally, stefin A and stefin B were measured in tumour and normal tissue samples. In contrast to the cathepsins, the concentrati …
Common polymorphism in the glycine N-methyltransferase gene as a novel risk factor for cleft lip with or without cleft palate.
Karas Kuželički N, Šmid A, Kek T, Eberlinc A, Geršak K, Mlinarič-Raščan I. Karas Kuželički N, et al. Among authors: smid a. Int J Oral Maxillofac Surg. 2018 Nov;47(11):1381-1388. doi: 10.1016/j.ijom.2018.06.001. Epub 2018 Jun 30. Int J Oral Maxillofac Surg. 2018. PMID: 30318092
Folate Insufficiency Due to MTHFR Deficiency Is Bypassed by 5-Methyltetrahydrofolate.
Vidmar Golja M, Šmid A, Karas Kuželički N, Trontelj J, Geršak K, Mlinarič-Raščan I. Vidmar Golja M, et al. Among authors: smid a. J Clin Med. 2020 Sep 2;9(9):E2836. doi: 10.3390/jcm9092836. J Clin Med. 2020. PMID: 32887268 Free article.
Short communication: Motivation to walk affects gait attributes.
Mokhtarnazif S, Smid AC, Weary DM, Mohamadnia A, von Keyserlingk MAG. Mokhtarnazif S, et al. Among authors: smid ac. J Dairy Sci. 2020 Oct;103(10):9481-9487. doi: 10.3168/jds.2019-18060. Epub 2020 Jul 31. J Dairy Sci. 2020. PMID: 32747100
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