Abstract
The self-transmissible plasmid pUO1 from Delftia acidovorans strain B carries two haloacetate-catabolic transposons, TnHad1 and TnHad2, and the mer genes for resistance to mercury. The complete 67,066-bp sequence of pUO1 revealed that the mer genes were also carried by two Tn402/Tn5053-like transposons, Tn4671 and Tn4672, and that the pUO1 backbone regions shared 99% identity to those of the archetype IncP-1beta plasmid R751. Comparison of pUO1 with three other IncP-1beta plasmids illustrated the importance of transposon insertion in the diversity and evolution of this group of plasmids. Mutational analysis of the four outermost residues in the inverted repeats (IRs) of TnHad2, a Tn21-related transposon, revealed a crucial role of the second residue of its IRs in transposition.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetates / chemistry
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Acetates / metabolism*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Base Sequence
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Biodegradation, Environmental
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Conjugation, Genetic*
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DNA Transposable Elements / genetics*
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DNA, Bacterial / genetics
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Delftia / drug effects
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Delftia / genetics*
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Delftia / metabolism
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Drug Resistance, Bacterial / genetics
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Escherichia coli / genetics
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Hydrolases / genetics*
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Hydrolases / metabolism
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Mercury / pharmacology
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Mutation
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Plasmids / genetics*
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Sequence Analysis, DNA
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Transposon Resolvases / genetics
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Transposon Resolvases / metabolism
Substances
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Acetates
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Bacterial Proteins
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DNA Transposable Elements
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DNA, Bacterial
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Tn21 resolvase
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Transposon Resolvases
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Hydrolases
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haloacetate dehalogenase
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Mercury