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A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies.
Ng ES, Davis R, Stanley EG, Elefanty AG. Ng ES, et al. Among authors: stanley eg. Nat Protoc. 2008;3(5):768-76. doi: 10.1038/nprot.2008.42. Nat Protoc. 2008. PMID: 18451785
Regulation of human embryonic stem cell differentiation by BMP-2 and its antagonist noggin.
Pera MF, Andrade J, Houssami S, Reubinoff B, Trounson A, Stanley EG, Ward-van Oostwaard D, Mummery C. Pera MF, et al. Among authors: stanley eg. J Cell Sci. 2004 Mar 1;117(Pt 7):1269-80. doi: 10.1242/jcs.00970. J Cell Sci. 2004. PMID: 14996946
Retinoic acid induces Pdx1-positive endoderm in differentiating mouse embryonic stem cells.
Micallef SJ, Janes ME, Knezevic K, Davis RP, Elefanty AG, Stanley EG. Micallef SJ, et al. Among authors: stanley eg. Diabetes. 2005 Feb;54(2):301-5. doi: 10.2337/diabetes.54.2.301. Epub 2004 Dec 7. Diabetes. 2005. PMID: 15585742
The primitive streak gene Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells.
Ng ES, Azzola L, Sourris K, Robb L, Stanley EG, Elefanty AG. Ng ES, et al. Among authors: stanley eg. Development. 2005 Mar;132(5):873-84. doi: 10.1242/dev.01657. Epub 2005 Jan 26. Development. 2005. PMID: 15673572
The hESC line Envy expresses high levels of GFP in all differentiated progeny.
Costa M, Dottori M, Ng E, Hawes SM, Sourris K, Jamshidi P, Pera MF, Elefanty AG, Stanley EG. Costa M, et al. Among authors: stanley eg. Nat Methods. 2005 Apr;2(4):259-60. doi: 10.1038/nmeth748. Epub 2005 Mar 23. Nat Methods. 2005. PMID: 15782217
Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation.
Ng ES, Davis RP, Azzola L, Stanley EG, Elefanty AG. Ng ES, et al. Among authors: stanley eg. Blood. 2005 Sep 1;106(5):1601-3. doi: 10.1182/blood-2005-03-0987. Epub 2005 May 24. Blood. 2005. PMID: 15914555
Mixl1 and oct4 proteins are transiently co-expressed in differentiating mouse and human embryonic stem cells.
Mossman AK, Sourris K, Ng E, Stanley EG, Elefanty AG. Mossman AK, et al. Among authors: stanley eg. Stem Cells Dev. 2005 Dec;14(6):656-63. doi: 10.1089/scd.2005.14.656. Stem Cells Dev. 2005. PMID: 16433620
Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene.
Hirst CE, Ng ES, Azzola L, Voss AK, Thomas T, Stanley EG, Elefanty AG. Hirst CE, et al. Among authors: stanley eg. Dev Biol. 2006 May 1;293(1):90-103. doi: 10.1016/j.ydbio.2006.01.023. Epub 2006 Mar 20. Dev Biol. 2006. PMID: 16546155
A method for genetic modification of human embryonic stem cells using electroporation.
Costa M, Dottori M, Sourris K, Jamshidi P, Hatzistavrou T, Davis R, Azzola L, Jackson S, Lim SM, Pera M, Elefanty AG, Stanley EG. Costa M, et al. Among authors: stanley eg. Nat Protoc. 2007;2(4):792-6. doi: 10.1038/nprot.2007.105. Nat Protoc. 2007. PMID: 17446878
Differentiation of human embryonic stem cells in serum-free medium reveals distinct roles for bone morphogenetic protein 4, vascular endothelial growth factor, stem cell factor, and fibroblast growth factor 2 in hematopoiesis.
Pick M, Azzola L, Mossman A, Stanley EG, Elefanty AG. Pick M, et al. Among authors: stanley eg. Stem Cells. 2007 Sep;25(9):2206-14. doi: 10.1634/stemcells.2006-0713. Epub 2007 Jun 7. Stem Cells. 2007. PMID: 17556598
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