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Biochemical analysis of the Saccharomyces cerevisiae SEC18 gene product: implications for the molecular mechanism of membrane fusion.
Steel GJ, Laude AJ, Boojawan A, Harvey DJ, Morgan A. Steel GJ, et al. Biochemistry. 1999 Jun 15;38(24):7764-72. doi: 10.1021/bi990315v. Biochemistry. 1999. PMID: 10387016
An in vitro assay using overexpressed yeast SRP demonstrates that cotranslational translocation is dependent upon the J-domain of Sec63p.
Willer M, Jermy AJ, Steel GJ, Garside HJ, Carter S, Stirling CJ. Willer M, et al. Among authors: steel gj. Biochemistry. 2003 Jun 17;42(23):7171-7. doi: 10.1021/bi034395l. Biochemistry. 2003. PMID: 12795613
Analysis of NSF mutants reveals residues involved in SNAP binding and ATPase stimulation.
Horsnell WG, Steel GJ, Morgan A. Horsnell WG, et al. Among authors: steel gj. Biochemistry. 2002 Apr 23;41(16):5230-5. doi: 10.1021/bi0160359. Biochemistry. 2002. PMID: 11955072
Tail-anchored protein insertion into yeast ER requires a novel posttranslational mechanism which is independent of the SEC machinery.
Steel GJ, Brownsword J, Stirling CJ. Steel GJ, et al. Biochemistry. 2002 Oct 1;41(39):11914-20. doi: 10.1021/bi026105r. Biochemistry. 2002. PMID: 12269836
Nucleotide binding by Lhs1p is essential for its nucleotide exchange activity and for function in vivo.
de Keyzer J, Steel GJ, Hale SJ, Humphries D, Stirling CJ. de Keyzer J, et al. Among authors: steel gj. J Biol Chem. 2009 Nov 13;284(46):31564-71. doi: 10.1074/jbc.M109.055160. Epub 2009 Sep 15. J Biol Chem. 2009. PMID: 19759005 Free PMC article.
Evidence for interaction of the fusion protein alpha-SNAP with membrane lipid.
Steel GJ, Buchheim G, Edwardson JM, Woodman PG. Steel GJ, et al. Biochem J. 1997 Jul 15;325 ( Pt 2)(Pt 2):511-8. doi: 10.1042/bj3250511. Biochem J. 1997. PMID: 9230135 Free PMC article.
Association of the fusion protein NSF with clathrin-coated vesicle membranes.
Steel GJ, Tagaya M, Woodman PG. Steel GJ, et al. EMBO J. 1996 Feb 15;15(4):745-52. EMBO J. 1996. PMID: 8631296 Free PMC article.
A screen for dominant negative mutants of SEC18 reveals a role for the AAA protein consensus sequence in ATP hydrolysis.
Steel GJ, Harley C, Boyd A, Morgan A. Steel GJ, et al. Mol Biol Cell. 2000 Apr;11(4):1345-56. doi: 10.1091/mbc.11.4.1345. Mol Biol Cell. 2000. PMID: 10749934 Free PMC article.
Selective stimulation of the D1 ATPase domain of N-ethylmaleimide-sensitive fusion protein (NSF) by soluble NSF attachment proteins.
Steel GJ, Morgan A. Steel GJ, et al. FEBS Lett. 1998 Feb 13;423(1):113-6. doi: 10.1016/s0014-5793(98)00072-6. FEBS Lett. 1998. PMID: 9506852 Free article.
Coordinated activation of Hsp70 chaperones.
Steel GJ, Fullerton DM, Tyson JR, Stirling CJ. Steel GJ, et al. Science. 2004 Jan 2;303(5654):98-101. doi: 10.1126/science.1092287. Science. 2004. PMID: 14704430 Free article.
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