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Calculation of the isoelectric point of tryptic peptides in the pH 3.5-4.5 range based on adjacent amino acid effects.
Cargile BJ, Sevinsky JR, Essader AS, Eu JP, Stephenson JL Jr. Cargile BJ, et al. Among authors: stephenson jl jr. Electrophoresis. 2008 Jul;29(13):2768-78. doi: 10.1002/elps.200700701. Electrophoresis. 2008. PMID: 18615785
Automated proteomics of E. coli via top-down electron-transfer dissociation mass spectrometry.
Bunger MK, Cargile BJ, Ngunjiri A, Bundy JL, Stephenson JL Jr. Bunger MK, et al. Among authors: stephenson jl jr. Anal Chem. 2008 Mar 1;80(5):1459-67. doi: 10.1021/ac7018409. Epub 2008 Jan 30. Anal Chem. 2008. PMID: 18229893
Whole genome searching with shotgun proteomic data: applications for genome annotation.
Sevinsky JR, Cargile BJ, Bunger MK, Meng F, Yates NA, Hendrickson RC, Stephenson JL Jr. Sevinsky JR, et al. Among authors: stephenson jl jr. J Proteome Res. 2008 Jan;7(1):80-8. doi: 10.1021/pr070198n. Epub 2007 Dec 7. J Proteome Res. 2008. PMID: 18062665
Detection and validation of non-synonymous coding SNPs from orthogonal analysis of shotgun proteomics data.
Bunger MK, Cargile BJ, Sevinsky JR, Deyanova E, Yates NA, Hendrickson RC, Stephenson JL Jr. Bunger MK, et al. Among authors: stephenson jl jr. J Proteome Res. 2007 Jun;6(6):2331-40. doi: 10.1021/pr0700908. Epub 2007 May 9. J Proteome Res. 2007. PMID: 17488105
Minimizing back exchange in 18O/16O quantitative proteomics experiments by incorporation of immobilized trypsin into the initial digestion step.
Sevinsky JR, Brown KJ, Cargile BJ, Bundy JL, Stephenson JL Jr. Sevinsky JR, et al. Among authors: stephenson jl jr. Anal Chem. 2007 Mar 1;79(5):2158-62. doi: 10.1021/ac0620819. Epub 2007 Jan 24. Anal Chem. 2007. PMID: 17249691 Free PMC article.
A comparison of immobilized pH gradient isoelectric focusing and strong-cation-exchange chromatography as a first dimension in shotgun proteomics.
Essader AS, Cargile BJ, Bundy JL, Stephenson JL Jr. Essader AS, et al. Among authors: stephenson jl jr. Proteomics. 2005 Jan;5(1):24-34. doi: 10.1002/pmic.200400888. Proteomics. 2005. PMID: 15672457
Immobilized pH gradients as a first dimension in shotgun proteomics and analysis of the accuracy of pI predictability of peptides.
Cargile BJ, Talley DL, Stephenson JL Jr. Cargile BJ, et al. Among authors: stephenson jl jr. Electrophoresis. 2004 Mar;25(6):936-45. doi: 10.1002/elps.200305722. Electrophoresis. 2004. PMID: 15004858
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