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Expression of human augmenter of liver regeneration in pichia pastoris yeast and its bioactivity in vitro.
Liu Q, Yu HF, Sun H, Ma HF. Liu Q, et al. Among authors: Sun H. World J Gastroenterol. 2004 Nov 1;10(21):3188-90. doi: 10.3748/wjg.v10.i21.3188. World J Gastroenterol. 2004. PMID: 15457571 Free PMC article.
The effects of hALR on in vitro proliferation of QGY and HepG(2) cells were evaluated by (3)H-TdR methods. RESULTS: The correctness and integrity of recombinant plasmids pPIC9-hALR and pPIC9K-hALR were identified by restriction digestion, PCR and sequencing methods, respectively. hALR as a secretive protein was successfully expressed by GS115. ...
The effects of hALR on in vitro proliferation of QGY and HepG(2) cells were evaluated by (3)H-TdR methods. RESULTS: The correctness a …
[Expression of CD14 protein in U937 cells induced by vitamin D3].
Sun H, Wu CX, Gong JP, Liu HZ, Li XH, You HB, Chen XF. Sun H, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Mar;21(2):155-8. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005. PMID: 15766397 Chinese.
[Inhibitory effect of blocking expression of human augmenter of liver regeneration (hALR) on proliferation of hepatocellular carcinoma cell line HepG2].
Tang L, Sun H, Zhang L, Guo H, Zhang L, Liu Q. Tang L, et al. Among authors: Sun H. Ai Zheng. 2006 Jun;25(6):671-6. Ai Zheng. 2006. PMID: 16764759 Chinese.
After transfection, the expression of green fluorescent protein was observed under a fluorescent microscope to calculate transfection efficiency, the protein level of hALR was measured by immunocytochemistry, meanwhile, the expression of hALR mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). (3)H-TdR incorporation approach was performed to detect the proliferation of HepG2 cells after transfection and after neutralization with anti-hALR McAb. ...Both immunocytochemistry and RT-PCR showed that pSIALR-A inhibited the expression of hALR in HepG2 cells significantly by 83% as compared with pSIALR-B. pSIALR-A specifically inhibited the growth of HepG2 cells after transfection. (3)H-TdR incorporation of pSIALR-A/HepG2 cells was significantly lower than that of pSIALR-B/HepG2 cells (67 687+/-6 548 vs. 104 807+/-5 713, P<0.05). ...
After transfection, the expression of green fluorescent protein was observed under a fluorescent microscope to calculate transfection effici …
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