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[Activation of low-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1 by heterologous activators].
Sheng Wu Gong Cheng Xue Bao. 2020 Aug 25;36(8):1578-1589. doi: 10.13345/j.cjb.190562.
Sheng Wu Gong Cheng Xue Bao. 2020.
PMID: 32924356
Free article.
Chinese.
Activator E and activator G had conserved domains (TIGR03889), and deletion of their partial sequences resulted in a substantial loss of activation abilities for both activators. ...
Activator E and activator G had conserved domains (TIGR03889), and deletion of their partial sequences resulted in a substantial loss …
Identification and functional analysis of the activator gene involved in the biosynthesis of Co-type nitrile hydratase from Aurantimonas manganoxydans.
Pei X, Yang Z, Wang A, Yang L, Wu J.
Pei X, et al.
J Biotechnol. 2017 Jun 10;251:38-46. doi: 10.1016/j.jbiotec.2017.03.016. Epub 2017 Mar 18.
J Biotechnol. 2017.
PMID: 28323014
Furthermore, the successive alpha-helices in domain TIGR03889 were identified to be critical for the activator function. The integration efficiency of cobalt into the active site was decreased with the interruption of alpha-helices. ...
Furthermore, the successive alpha-helices in domain TIGR03889 were identified to be critical for the activator function. The integrat …
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