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A method to obtain avian germ-line chimaeras using isolated primordial germ cells.
Yasuda Y, Tajima A, Fujimoto T, Kuwana T. Yasuda Y, et al. J Reprod Fertil. 1992 Nov;96(2):521-8. doi: 10.1530/jrf.0.0960521. J Reprod Fertil. 1992. PMID: 1339832
The PGCs were picked up with a fine glass pipette, and one hundred were then injected into the terminal sinuses of 2-day-old Japanese quail embryos (24 somites); bubbles were then inserted to prevent haemorrhage. ...A similar percentage of the host (quail) PGCs had …
The PGCs were picked up with a fine glass pipette, and one hundred were then injected into the terminal sinuses of 2-day-old Japanese …
Simple method for isolation of primordial germ cells from chick embryos.
Chang IK, Tajima A, Yasuda Y, Chikamune T, Ohno T. Chang IK, et al. Cell Biol Int Rep. 1992 Sep;16(9):853-7. doi: 10.1016/s0309-1651(06)80165-7. Cell Biol Int Rep. 1992. PMID: 1423655
A simple one step centrifugation method was developed for purification of primordial germ cells (PGCs) of chick embryos. PGCs, constituting less than 0.1% of the total blood cells of stage 13-14 embryos that contained a microliter amount of blood, were concentrated
A simple one step centrifugation method was developed for purification of primordial germ cells (PGCs) of chick embryos. PGCs, consti
Germ line chimera produced by transfer of cultured chick primordial germ cells.
Chang IK, Yoshiki A, Kusakabe M, Tajima A, Chikamune T, Naito M, Ohno T. Chang IK, et al. Cell Biol Int. 1995 Jul;19(7):569-76. doi: 10.1006/cbir.1995.1103. Cell Biol Int. 1995. PMID: 7550064
Intrinsic primordial germ cells (PGCs) from stage 27 (5-day-old) chick embryonic germinal ridges were cultured in vitro for a further 5 days, and shown to proliferate on stroma cells derived from the germinal ridge. ...
Intrinsic primordial germ cells (PGCs) from stage 27 (5-day-old) chick embryonic germinal ridges were cultured in vitro for a further …
Preservation of chick primordial germ cells in liquid nitrogen and subsequent production of viable offspring.
Naito M, Tajima A, Tagami T, Yasuda Y, Kuwana T. Naito M, et al. J Reprod Fertil. 1994 Nov;102(2):321-5. doi: 10.1530/jrf.0.1020321. J Reprod Fertil. 1994. PMID: 7861384
The primordial germ cells were then suspended in a freezing medium containing 10% dimethyl sulfoxide. The temperature of the cell suspension was decreased by 1 degree C min-1 to -80 degrees C; the suspension was then placed in liquid nitrogen (-196 degrees C) and stored fo …
The primordial germ cells were then suspended in a freezing medium containing 10% dimethyl sulfoxide. The temperature of the cell sus …
Production of germ-line chimeras by transfer of cryopreserved gonadal primordial germ cells (gPGCs) in chicken.
Tajima A, Naito M, Yasuda Y, Kuwana T. Tajima A, et al. J Exp Zool. 1998 Feb 15;280(3):265-7. J Exp Zool. 1998. PMID: 9472483
Upon reaching sexual maturity, a progeny test was performed by mating recipient chicks with normal BPR of the opposite sex. Of 840 progeny produced from 4 gPGC recipients, 74 chicks (8.8%) were phenotypically white. ...
Upon reaching sexual maturity, a progeny test was performed by mating recipient chicks with normal BPR of the opposite sex. Of 840 pr …
Donor primordial germ cell-derived offspring from recipient germline chimaeric chickens: absence of long-term immune rejection and effects on sex ratios.
Naito M, Tajima A, Yasuda Y, Kuwana T. Naito M, et al. Br Poult Sci. 1998 Mar;39(1):20-3. doi: 10.1080/00071669889330. Br Poult Sci. 1998. PMID: 9568293
Germline chimaeric chickens were produced by the transfer of primordial germ cells, and the generation of donor-derived offspring was examined for a maximum of 146 weeks. 2. ...Differentiation of donor primordial germ cells into functional gametes appeared to be restricted …
Germline chimaeric chickens were produced by the transfer of primordial germ cells, and the generation of donor-derived offspring was examin …
Study on the concentration of circulating primordial germ cells (cPGCs) in early chick embryos.
Tajima A, Hayashi H, Kamizumi A, Ogura J, Kuwana T, Chikamune T. Tajima A, et al. J Exp Zool. 1999 Dec 1;284(7):759-64. doi: 10.1002/(sici)1097-010x(19991201)284:7<759::aid-jez5>3.0.co;2-6. J Exp Zool. 1999. PMID: 10589506
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