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Quantitative determination of isothiocyanates, dithiocarbamates, carbon disulfide, and related thiocarbonyl compounds by cyclocondensation with 1,2-benzenedithiol.
Zhang Y, Wade KL, Prestera T, Talalay P. Zhang Y, et al. Anal Biochem. 1996 Aug 1;239(2):160-7. doi: 10.1006/abio.1996.0311. Anal Biochem. 1996. PMID: 8811900
Posner, and P. Talalay, Anal. Biochem. 205, 100-107, 1992). The assay utilizes 1, 2-benzenedithiol as the vicinal dithiol reagent and measures the reaction product, 1,3-benzodithiole-2-thione, spectroscopically (am of 23,000 M-1 cm-1 at lambdamax = 365 nm). ...
Posner, and P. Talalay, Anal. Biochem. 205, 100-107, 1992). The assay utilizes 1, 2-benzenedithiol as the vicinal dithiol reag …
Positioning of a spin-labeled substrate analogue into the structure of delta 5-3-ketosteroid isomerase by combined kinetic, magnetic resonance, and X-ray diffraction methods.
Kuliopulos A, Westbrook EM, Talalay P, Mildvan AS. Kuliopulos A, et al. Biochemistry. 1987 Jun 30;26(13):3927-37. doi: 10.1021/bi00387a028. Biochemistry. 1987. PMID: 2888482
., & Sigler, P. B. (1984) J. Biol. Chem. 259, 9096-9103]. However, the rotational orientation of this steroid about its long axis could not be unambiguously established. ...
., & Sigler, P. B. (1984) J. Biol. Chem. 259, 9096-9103]. However, the rotational orientation of this steroid about its long axis …
Kinetic and ultraviolet spectroscopic studies of active-site mutants of delta 5-3-ketosteroid isomerase.
Kuliopulos A, Mildvan AS, Shortle D, Talalay P. Kuliopulos A, et al. Biochemistry. 1989 Jan 10;28(1):149-59. doi: 10.1021/bi00427a022. Biochemistry. 1989. PMID: 2706241
., Talalay, P., & Mildvan, A.S. (1987) Biochemistry 26, 3927-3937], thereby corroborating the approximate location of the steroid binding site deduced from a difference Fourier X-ray diffraction map of the 4-(acetoxymercuri)estradiol-isomerase complex [Westbrook …
., Talalay, P., & Mildvan, A.S. (1987) Biochemistry 26, 3927-3937], thereby corroborating the approximate location of the …
Combined effects of two mutations of catalytic residues on the ketosteroid isomerase reaction.
Kuliopulos A, Talalay P, Mildvan AS. Kuliopulos A, et al. Biochemistry. 1990 Nov 6;29(44):10271-80. doi: 10.1021/bi00496a017. Biochemistry. 1990. PMID: 2271654
., & Talalay, P. (1989) Biochemistry 28, 149-159]. Primary, secondary, and combined deuterium kinetic isotope effects establish concerted substrate enolization to be the rate-limiting step with the wild-type enzyme [Xue, L., Talalay, P., & Mild …
., & Talalay, P. (1989) Biochemistry 28, 149-159]. Primary, secondary, and combined deuterium kinetic isotope effects esta …
Studies of the catalytic mechanism of an active-site mutant (Y14F) of delta 5-3-ketosteroid isomerase by kinetic deuterium isotope effects.
Xue LA, Talalay P, Mildvan AS. Xue LA, et al. Biochemistry. 1991 Nov 12;30(45):10858-65. doi: 10.1021/bi00109a008. Biochemistry. 1991. PMID: 1932008
., Talalay, P., & Mildvan, A. S. (1990) Biochemistry 29, 7491-7500]. Primary, secondary, solvent, and combined kinetic deuterium isotope effects have been used to investigate the mechanism of the Y14F mutant, which lacks the proton donor and is 10(4.7)-fold less …
., Talalay, P., & Mildvan, A. S. (1990) Biochemistry 29, 7491-7500]. Primary, secondary, solvent, and combined kinetic deu …
Quantitative determination of dithiocarbamates in human plasma, serum, erythrocytes and urine: pharmacokinetics of broccoli sprout isothiocyanates in humans.
Ye L, Dinkova-Kostova AT, Wade KL, Zhang Y, Shapiro TA, Talalay P. Ye L, et al. Clin Chim Acta. 2002 Feb;316(1-2):43-53. doi: 10.1016/s0009-8981(01)00727-6. Clin Chim Acta. 2002. PMID: 11750273
Chemoprotective glucosinolates and isothiocyanates of broccoli sprouts: metabolism and excretion in humans.
Shapiro TA, Fahey JW, Wade KL, Stephenson KK, Talalay P. Shapiro TA, et al. Cancer Epidemiol Biomarkers Prev. 2001 May;10(5):501-8. Cancer Epidemiol Biomarkers Prev. 2001. PMID: 11352861 Clinical Trial.

Cumulative excretion of dithiocarbamates following 111-micromol doses of isothiocyanates was greater than that after glucosinolates (88.9 +/- 5.5 and 13.1 +/- 1.9 micromol, respectively; P < 0.0003). ...Dithiocarbamate excretion was higher when intact sprouts were chewe

Cumulative excretion of dithiocarbamates following 111-micromol doses of isothiocyanates was greater than that after glucosinolates (88.9 +/ …
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