We present a procedure for optimizing the expression of recombinant tetrameric butyrylcholinesterase that enables large-scale production with the yield >30 mg/liter (>90 mg/roller bottle). Intravenous injection of the preparation significantly increased survival and decreased the severity of symptoms of poisoning with paraoxon, an organophosphorus toxin.
Keywords: biological drug; organophosphate toxins; paraoxon; prevention of poisoning; tetrameric recombinant human butyrylcholinesterase.