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Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.
Chiu J, March PE, Lee R, Tillett D. Chiu J, et al. Among authors: tillett d. Nucleic Acids Res. 2004 Dec 7;32(21):e174. doi: 10.1093/nar/gnh172. Nucleic Acids Res. 2004. PMID: 15585660 Free PMC article.
Mutation of Phe102 to Ser in the carboxyl terminal helix of Escherichia coli thioredoxin affects the stability and processivity of T7 DNA polymerase.
Chiu J, Tillett D, March PE. Chiu J, et al. Among authors: tillett d. Proteins. 2006 Aug 1;64(2):477-85. doi: 10.1002/prot.20992. Proteins. 2006. PMID: 16671068
Site-directed, Ligase-Independent Mutagenesis (SLIM) for highly efficient mutagenesis of plasmids greater than 8kb.
Chiu J, Tillett D, Dawes IW, March PE. Chiu J, et al. Among authors: tillett d. J Microbiol Methods. 2008 May;73(2):195-8. doi: 10.1016/j.mimet.2008.02.013. Epub 2008 Feb 26. J Microbiol Methods. 2008. PMID: 18387684
Primer fabrication using polymerase mediated oligonucleotide synthesis.
Cairns MJ, Thomas T, Beltran CE, Tillett D. Cairns MJ, et al. Among authors: tillett d. BMC Genomics. 2009 Jul 31;10:344. doi: 10.1186/1471-2164-10-344. BMC Genomics. 2009. PMID: 19643029 Free PMC article.
Coexpression of the subunits of T7 DNA polymerase from an artificial operon allows one-step purification of active gp5/Trx complex.
Chiu J, Tillett D, March PE. Chiu J, et al. Among authors: tillett d. Protein Expr Purif. 2006 May;47(1):264-72. doi: 10.1016/j.pep.2005.10.016. Epub 2005 Nov 8. Protein Expr Purif. 2006. PMID: 16300964
Enzyme-free cloning of PCR products and fusion protein expression.
Neilan BA, Tillett D. Neilan BA, et al. Among authors: tillett d. Methods Mol Biol. 2002;192:125-32. doi: 10.1385/1-59259-177-9:125. Methods Mol Biol. 2002. PMID: 12494645 No abstract available.
Optimized rapid amplification of cDNA ends (RACE) for mapping bacterial mRNA transcripts.
Tillett D, Burns BP, Neilan BA. Tillett D, et al. Biotechniques. 2000 Mar;28(3):448, 450, 452-3, 456. doi: 10.2144/00283st01. Biotechniques. 2000. PMID: 10723556
Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites.
Tillett D, Neilan BA. Tillett D, et al. Nucleic Acids Res. 1999 Oct 1;27(19):e26. doi: 10.1093/nar/27.19.e26. Nucleic Acids Res. 1999. PMID: 10481038 Free PMC article.
n-butanol purification of dye terminator sequencing reactions.
Tillett D, Neilan BA. Tillett D, et al. Biotechniques. 1999 Apr;26(4):606-8, 610. doi: 10.2144/99264bm02. Biotechniques. 1999. PMID: 10343893 No abstract available.
A novel method of extracting plasmid DNA from Helicobacter species.
De Ungria MC, Tillett D, Neilan BA, Cox PT, Lee A. De Ungria MC, et al. Among authors: tillett d. Helicobacter. 1998 Dec;3(4):269-77. doi: 10.1111/j.1523-5378.1997.06085.pp.x-i1. Helicobacter. 1998. PMID: 9844068
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