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A dual lethal system to enhance containment of recombinant micro-organisms.
Torres B, Jaenecke S, Timmis KN, García JL, Díaz E. Torres B, et al. Microbiology. 2003 Dec;149(Pt 12):3595-3601. doi: 10.1099/mic.0.26618-0. Microbiology. 2003. PMID: 14663091
Metagenomics for mining new genetic resources of microbial communities.
Ferrer M, Beloqui A, Timmis KN, Golyshin PN. Ferrer M, et al. J Mol Microbiol Biotechnol. 2009;16(1-2):109-23. doi: 10.1159/000142898. Epub 2008 Oct 29. J Mol Microbiol Biotechnol. 2009. PMID: 18957866 Review.
A gene containment strategy based on a restriction-modification system.
Torres B, Jaenecke S, Timmis KN, García JL, Díaz E. Torres B, et al. Environ Microbiol. 2000 Oct;2(5):555-63. doi: 10.1046/j.1462-2920.2000.00138.x. Environ Microbiol. 2000. PMID: 11233163
The behavior of bacteria designed for biodegradation.
Ramos JL, Díaz E, Dowling D, de Lorenzo V, Molin S, O'Gara F, Ramos C, Timmis KN. Ramos JL, et al. Biotechnology (N Y). 1994 Dec;12(13):1349-56. doi: 10.1038/nbt1294-1349. Biotechnology (N Y). 1994. PMID: 7765565 Free PMC article. Review.
Early and late responses of TOL promoters to pathway inducers: identification of postexponential promoters in Pseudomonas putida with lacZ-tet bicistronic reporters.
de Lorenzo V, Cases I, Herrero M, Timmis KN. de Lorenzo V, et al. J Bacteriol. 1993 Nov;175(21):6902-7. doi: 10.1128/jb.175.21.6902-6907.1993. J Bacteriol. 1993. PMID: 8226632 Free PMC article.
Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria.
de Lorenzo V, Herrero M, Jakubzik U, Timmis KN. de Lorenzo V, et al. J Bacteriol. 1990 Nov;172(11):6568-72. doi: 10.1128/jb.172.11.6568-6572.1990. J Bacteriol. 1990. PMID: 2172217 Free PMC article.
Mutations leading to constitutive expression from the TOL plasmid meta-cleavage pathway operon are located at the C-terminal end of the positive regulator protein XylS.
Zhou LM, Timmis KN, Ramos JL. Zhou LM, et al. J Bacteriol. 1990 Jul;172(7):3707-10. doi: 10.1128/jb.172.7.3707-3710.1990. J Bacteriol. 1990. PMID: 2193914 Free PMC article.
Engineering of alkyl- and haloaromatic-responsive gene expression with mini-transposons containing regulated promoters of biodegradative pathways of Pseudomonas.
de Lorenzo V, Fernández S, Herrero M, Jakubzik U, Timmis KN. de Lorenzo V, et al. Gene. 1993 Aug 16;130(1):41-6. doi: 10.1016/0378-1119(93)90344-3. Gene. 1993. PMID: 8393826
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