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The evolution of pandemic clones of methicillin-resistant Staphylococcus aureus: identification of two ancestral genetic backgrounds and the associated mec elements.
Oliveira DC, Tomasz A, de Lencastre H. Oliveira DC, et al. Microb Drug Resist. 2001 Winter;7(4):349-61. doi: 10.1089/10766290152773365. Microb Drug Resist. 2001. PMID: 11822775
Three of the previously classified clonal types (Iberian, Brazilian, and Hungarian clones) shared a common or closely related genetic background A, which was the same as the background of the earliest European isolates of MRSA from England and Denmark. ...A n …
Three of the previously classified clonal types (Iberian, Brazilian, and Hungarian clones) shared a common or closely related genetic …
Beta-lactam-specific resistant mutants of Staphylococcus aureus.
Tonin E, Tomasz A. Tonin E, et al. Antimicrob Agents Chemother. 1986 Oct;30(4):577-83. doi: 10.1128/aac.30.4.577. Antimicrob Agents Chemother. 1986. PMID: 3539012 Free PMC article.
In a highly methicillin-resistant mutant (MIC, 150 micrograms/ml), PBPs 2 and 3 were no longer detectable by the penicillin binding assay. Instead, a new PBP of poor binding capacity and anomalous molecular size (about 78 kilodaltons [kDa]) appeared in these cells. …
In a highly methicillin-resistant mutant (MIC, 150 micrograms/ml), PBPs 2 and 3 were no longer detectable by the penicillin binding a …
Altered penicillin-binding proteins in methicillin-resistant strains of Staphylococcus aureus.
Hartman B, Tomasz A. Hartman B, et al. Antimicrob Agents Chemother. 1981 May;19(5):726-35. doi: 10.1128/aac.19.5.726. Antimicrob Agents Chemother. 1981. PMID: 7294764 Free PMC article.
The penicillin-binding proteins (PBPs) of a methicillin-resistant (MR) and a methicillin-susceptible (MS) Staphylococcus aureus were compared by various approaches involving the use of high-specific-activity [3H]penicillin as a reagent. ...Thus, the pH-depend …
The penicillin-binding proteins (PBPs) of a methicillin-resistant (MR) and a methicillin-susceptible (MS) Staphylococcus aureu …
Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus.
Hartman BJ, Tomasz A. Hartman BJ, et al. J Bacteriol. 1984 May;158(2):513-6. doi: 10.1128/JB.158.2.513-516.1984. J Bacteriol. 1984. PMID: 6563036 Free PMC article.
We detected a high-molecular-weight PBP (PBP-2a; approximate size, 78,000 daltons) that was only present in the resistant bacteria but not in the isogenic sensitive strain. ...
We detected a high-molecular-weight PBP (PBP-2a; approximate size, 78,000 daltons) that was only present in the resistant bacteria bu …
Increased amounts of a novel penicillin-binding protein in a strain of methicillin-resistant Staphylococcus aureus exposed to nafcillin.
Chambers HF, Hartman BJ, Tomasz A. Chambers HF, et al. J Clin Invest. 1985 Jul;76(1):325-31. doi: 10.1172/JCI111965. J Clin Invest. 1985. PMID: 4019783 Free PMC article.
In addition to the four typical penicillin-binding proteins (PBPs), a strain of heterogeneously methicillin-resistant Staphylococcus aureus produced an extra 78-kD PBP (PBP 2a) that had a low affinity for nafcillin and penicillin. Addition of nafcillin to cultures o …
In addition to the four typical penicillin-binding proteins (PBPs), a strain of heterogeneously methicillin-resistant Staphylococcus …
Peptidoglycan composition of a highly methicillin-resistant Staphylococcus aureus strain. The role of penicillin binding protein 2A.
de Jonge BL, Chang YS, Gage D, Tomasz A. de Jonge BL, et al. J Biol Chem. 1992 Jun 5;267(16):11248-54. J Biol Chem. 1992. PMID: 1597460
This extra PBP is thought to be a transpeptidase essential for the continued cell wall synthesis and growth in the presence of beta-lactam antibiotics. ...Our experiments provided no experimental data for a unique transpeptidase activity associated with PBP2A....
This extra PBP is thought to be a transpeptidase essential for the continued cell wall synthesis and growth in the presence of beta-l …
Abnormal physiological properties and altered cell wall composition in Streptococcus pneumoniae grown in the presence of clavulanic acid.
Severin A, Severina E, Tomasz A. Severin A, et al. Antimicrob Agents Chemother. 1997 Mar;41(3):504-10. doi: 10.1128/AAC.41.3.504. Antimicrob Agents Chemother. 1997. PMID: 9055983 Free PMC article.
Naturally occurring peptidoglycan variants of Streptococcus pneumoniae.
Severin A, Tomasz A. Severin A, et al. J Bacteriol. 1996 Jan;178(1):168-74. doi: 10.1128/jb.178.1.168-174.1996. J Bacteriol. 1996. PMID: 8550412 Free PMC article.
Analysis by high-performance liquid chromatography of the stem peptide composition of cell walls purified from a large number of pneumococcal strains indicates that these bacteria produce a highly conserved species-specific peptidoglycan independent of serotype, iso …
Analysis by high-performance liquid chromatography of the stem peptide composition of cell walls purified from a large number of pneu …
The apparent importation of penicillin-resistant capsular type 14 Spanish/French clone of Streptococcus pneumoniae into Uruguay in the early 1990s.
Camou T, Hortal M, Tomasz A. Camou T, et al. Microb Drug Resist. 1998 Fall;4(3):219-24. doi: 10.1089/mdr.1998.4.219. Microb Drug Resist. 1998. PMID: 9818973
One of the subtypes (B12) was indistinguishable from a French isolate (M13P). This strain represented a South European clone, identified in the late 1980s, that also included serogroup 9 isolates. ...Follow-up of this phenomenon is mandatory from the point of view o …
One of the subtypes (B12) was indistinguishable from a French isolate (M13P). This strain represented a South European clone, …
Antibiotic susceptibility of Streptococcus pneumoniae isolates in Portugal. A multicenter study between 1989 and 1993.
Pato MV, Carvalho CB, Tomasz A. Pato MV, et al. Microb Drug Resist. 1995 Spring;1(1):59-69. doi: 10.1089/mdr.1995.1.59. Microb Drug Resist. 1995. PMID: 9156385 Clinical Trial.
A total of 1,104 strains of Streptococcus pneumoniae were isolated from hospitalized patients (75.5%) and from colonization sites at the Public Health Institute (24.5%) during the last 4 years (1989-1993). ...
A total of 1,104 strains of Streptococcus pneumoniae were isolated from hospitalized patients (75.5%) and from colonization sites at
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