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Establishment of a cell line (HCC-M) from a human hepatocellular carcinoma.
Watanabe T, et al. Int J Cancer 1983. Among authors: Tsuchiya M. PMID 6307892
The cell line, designated HCC-M, grows as an adhering monolayer with a doubling time of 24 h in medium RPMI-1640 supplemented with 10% FCS and grows with 30% clonal efficiency in soft agar. ...HCC-M had a chromosome mode of 63 with five identifiable marker chromosomes. HCC-M produced albumin at the 10th passage but this property was lost by the 30th passage. ...
The cell line, designated HCC-M, grows as an adhering monolayer with a doubling time of 24 h in medium RPMI-1640 supplemented with 10 …
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