Abstract
A 3.1 kb fragment of Bacillus stearothermophilus ATCC12980 DNA permitted growth of Escherichia coli on sucrose. The fragment encoded genes for a levansucrase (surB) and also a levanase (surC). SurB and SurC shared 97% and 43% amino acid identity with the corresponding SacB and SacC proteins of Bacillus subtilis, whose sacB and sacC genes are organised very differently.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins*
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Base Sequence
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Cloning, Molecular
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Genes, Bacterial / genetics*
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Geobacillus stearothermophilus / enzymology
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Geobacillus stearothermophilus / genetics*
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Glycoside Hydrolases / genetics*
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Hexosyltransferases / genetics*
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Molecular Sequence Data
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Multigene Family / genetics*
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Open Reading Frames / genetics
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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Bacterial Proteins
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Hexosyltransferases
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levansucrase
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Glycoside Hydrolases
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levanase
Associated data
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GENBANK/U34874
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GENBANK/U34875