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Functional metagenomics for enzyme discovery: challenges to efficient screening.
Uchiyama T, Miyazaki K. Uchiyama T, et al. Curr Opin Biotechnol. 2009 Dec;20(6):616-22. doi: 10.1016/j.copbio.2009.09.010. Epub 2009 Oct 21. Curr Opin Biotechnol. 2009. PMID: 19850467 Review.
Product-induced gene expression, a product-responsive reporter assay used to screen metagenomic libraries for enzyme-encoding genes.
Uchiyama T, Miyazaki K. Uchiyama T, et al. Appl Environ Microbiol. 2010 Nov;76(21):7029-35. doi: 10.1128/AEM.00464-10. Epub 2010 Sep 10. Appl Environ Microbiol. 2010. PMID: 20833789 Free PMC article.
Metagenomic screening for aromatic compound-responsive transcriptional regulators.
Uchiyama T, Miyazaki K. Uchiyama T, et al. PLoS One. 2013 Sep 30;8(9):e75795. doi: 10.1371/journal.pone.0075795. eCollection 2013. PLoS One. 2013. PMID: 24098725 Free PMC article.
Improved inverse PCR scheme for metagenome walking.
Uchiyama T, Watanabe K. Uchiyama T, et al. Biotechniques. 2006 Aug;41(2):183-8. doi: 10.2144/000112210. Biotechniques. 2006. PMID: 16925020
Isolation and functional analysis of cytochrome P450 CYP153A genes from various environments.
Kubota M, Nodate M, Yasumoto-Hirose M, Uchiyama T, Kagami O, Shizuri Y, Misawa N. Kubota M, et al. Biosci Biotechnol Biochem. 2005 Dec;69(12):2421-30. doi: 10.1271/bbb.69.2421. Biosci Biotechnol Biochem. 2005. PMID: 16377903
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