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A Protein Epitope Signature Tag (PrEST) library allows SILAC-based absolute quantification and multiplexed determination of protein copy numbers in cell lines.
Zeiler M, Straube WL, Lundberg E, Uhlen M, Mann M. Zeiler M, et al. Mol Cell Proteomics. 2012 Mar;11(3):O111.009613. doi: 10.1074/mcp.O111.009613. Epub 2011 Sep 30. Mol Cell Proteomics. 2012. PMID: 21964433 Free PMC article.
Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues.
Agaton C, Galli J, Höidén Guthenberg I, Janzon L, Hansson M, Asplund A, Brundell E, Lindberg S, Ruthberg I, Wester K, Wurtz D, Höög C, Lundeberg J, Ståhl S, Pontén F, Uhlén M. Agaton C, et al. Mol Cell Proteomics. 2003 Jun;2(6):405-14. doi: 10.1074/mcp.M300022-MCP200. Epub 2003 Jun 9. Mol Cell Proteomics. 2003. PMID: 12796447
Antibody-based proteomics for human tissue profiling.
Uhlen M, Ponten F. Uhlen M, et al. Mol Cell Proteomics. 2005 Apr;4(4):384-93. doi: 10.1074/mcp.R500009-MCP200. Epub 2005 Feb 5. Mol Cell Proteomics. 2005. PMID: 15695805 Review.
A human protein atlas for normal and cancer tissues based on antibody proteomics.
Uhlén M, Björling E, Agaton C, Szigyarto CA, Amini B, Andersen E, Andersson AC, Angelidou P, Asplund A, Asplund C, Berglund L, Bergström K, Brumer H, Cerjan D, Ekström M, Elobeid A, Eriksson C, Fagerberg L, Falk R, Fall J, Forsberg M, Björklund MG, Gumbel K, Halimi A, Hallin I, Hamsten C, Hansson M, Hedhammar M, Hercules G, Kampf C, Larsson K, Lindskog M, Lodewyckx W, Lund J, Lundeberg J, Magnusson K, Malm E, Nilsson P, Odling J, Oksvold P, Olsson I, Oster E, Ottosson J, Paavilainen L, Persson A, Rimini R, Rockberg J, Runeson M, Sivertsson A, Sköllermo A, Steen J, Stenvall M, Sterky F, Strömberg S, Sundberg M, Tegel H, Tourle S, Wahlund E, Waldén A, Wan J, Wernérus H, Westberg J, Wester K, Wrethagen U, Xu LL, Hober S, Pontén F. Uhlén M, et al. Mol Cell Proteomics. 2005 Dec;4(12):1920-32. doi: 10.1074/mcp.M500279-MCP200. Epub 2005 Aug 27. Mol Cell Proteomics. 2005. PMID: 16127175
Serum microarrays for large scale screening of protein levels.
Janzi M, Odling J, Pan-Hammarström Q, Sundberg M, Lundeberg J, Uhlén M, Hammarström L, Nilsson P. Janzi M, et al. Mol Cell Proteomics. 2005 Dec;4(12):1942-7. doi: 10.1074/mcp.M500213-MCP200. Epub 2005 Aug 29. Mol Cell Proteomics. 2005. PMID: 16131484
Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling.
Nilsson P, Paavilainen L, Larsson K, Odling J, Sundberg M, Andersson AC, Kampf C, Persson A, Al-Khalili Szigyarto C, Ottosson J, Björling E, Hober S, Wernérus H, Wester K, Pontén F, Uhlen M. Nilsson P, et al. Proteomics. 2005 Nov;5(17):4327-37. doi: 10.1002/pmic.200500072. Proteomics. 2005. PMID: 16237735
From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Ek S, Andréasson U, Hober S, Kampf C, Pontén F, Uhlén M, Merz H, Borrebaeck CA. Ek S, et al. Mol Cell Proteomics. 2006 Jun;5(6):1072-81. doi: 10.1074/mcp.M600077-MCP200. Epub 2006 Mar 8. Mol Cell Proteomics. 2006. PMID: 16524965
Multiplexed PrEST immunization for high-throughput affinity proteomics.
Larsson K, Wester K, Nilsson P, Uhlén M, Hober S, Wernérus H. Larsson K, et al. J Immunol Methods. 2006 Aug 31;315(1-2):110-20. doi: 10.1016/j.jim.2006.07.014. Epub 2006 Aug 17. J Immunol Methods. 2006. PMID: 16949094
A web-based tool for in silico biomarker discovery based on tissue-specific protein profiles in normal and cancer tissues.
Björling E, Lindskog C, Oksvold P, Linné J, Kampf C, Hober S, Uhlén M, Pontén F. Björling E, et al. Mol Cell Proteomics. 2008 May;7(5):825-44. doi: 10.1074/mcp.M700411-MCP200. Epub 2007 Oct 3. Mol Cell Proteomics. 2008. PMID: 17913849
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