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High-temperature, but not high-pressure, conditions alter neuronal activity.
Mizunuma M, Takahashi N, Usami A, Matsuki N, Ikegaya Y. Mizunuma M, et al. Among authors: usami a. J Pharmacol Sci. 2009 May;110(1):117-21. doi: 10.1254/jphs.09031sc. Epub 2009 May 9. J Pharmacol Sci. 2009. PMID: 19430196
Increased intracranial pressure is generally a pathological sign observed in intracerebral hemorrhage, brain edema, and brain tumor, yet little is known about how the hyperbaric pressure per se affects neuronal activity. ...Thus, neurons are sensitive to feverish condition …
Increased intracranial pressure is generally a pathological sign observed in intracerebral hemorrhage, brain edema, and brain tumor, …
Hippocampal polysynaptic computation.
Kimura R, Kang S, Takahashi N, Usami A, Matsuki N, Fukai T, Ikegaya Y. Kimura R, et al. Among authors: usami a. J Neurosci. 2011 Sep 14;31(37):13168-79. doi: 10.1523/JNEUROSCI.1920-11.2011. J Neurosci. 2011. PMID: 21917800 Free PMC article.
Neural circuitry is a self-organizing arithmetic device that converts input to output and thereby remodels its computational algorithm to produce more desired output; however, experimental evidence regarding the mechanism by which information is modified and stored while p …
Neural circuitry is a self-organizing arithmetic device that converts input to output and thereby remodels its computational algorith …
Fluorescent pipettes for optically targeted patch-clamp recordings.
Ishikawa D, Takahashi N, Sasaki T, Usami A, Matsuki N, Ikegaya Y. Ishikawa D, et al. Among authors: usami a. Neural Netw. 2010 Aug;23(6):669-72. doi: 10.1016/j.neunet.2010.02.004. Epub 2010 Feb 24. Neural Netw. 2010. PMID: 20223634
Targeted patch-clamp recordings are a promising technique that can directly address the physiological properties of a specific neuron embedded in a neuronal network. ...Here we introduce a simpler method for neuron targeting. With fluorophore-coated pi …
Targeted patch-clamp recordings are a promising technique that can directly address the physiological properties of a specific …
Spontaneous plasticity of multineuronal activity patterns in activated hippocampal networks.
Usami A, Matsuki N, Ikegaya Y. Usami A, et al. Neural Plast. 2008;2008:108969. doi: 10.1155/2008/108969. Neural Plast. 2008. PMID: 18645610 Free PMC article.
Thus, the networks can modify themselves by altering the internal structure of their correlated activity, even though they as a whole maintained the same level of activity in space and time....
Thus, the networks can modify themselves by altering the internal structure of their correlated activity, even though they as a whole …
Oseltamivir enhances hippocampal network synchronization.
Usami A, Sasaki T, Satoh N, Akiba T, Yokoshima S, Fukuyama T, Yamatsugu K, Kanai M, Shibasaki M, Matsuki N, Ikegaya Y. Usami A, et al. J Pharmacol Sci. 2008 Apr;106(4):659-62. doi: 10.1254/jphs.sc0070467. Epub 2008 Apr 9. J Pharmacol Sci. 2008. PMID: 18403897
Oseltamivir, a widely used anti-influenza drug, inhibits virus neuraminidase. A mammalian homologue of this enzyme is expressed in the brain, yet the effect of oseltamivir on central neurons is largely unknown. ...Thus, oseltamivir is a useful tool for invest …
Oseltamivir, a widely used anti-influenza drug, inhibits virus neuraminidase. A mammalian homologue of this enzyme is expresse …
Active hippocampal networks undergo spontaneous synaptic modification.
Tsukamoto-Yasui M, Sasaki T, Matsumoto W, Hasegawa A, Toyoda T, Usami A, Kubota Y, Ochiai T, Hori T, Matsuki N, Ikegaya Y. Tsukamoto-Yasui M, et al. Among authors: usami a. PLoS One. 2007 Nov 28;2(11):e1250. doi: 10.1371/journal.pone.0001250. PLoS One. 2007. PMID: 18043757 Free PMC article.
The spontaneous synaptic plasticity depended on a rise in intracellular calcium concentrations of postsynaptic cells, but not on NMDA receptor activity. ...With computational simulations, we suggest that with this slow oscillation-induced plasticity, a recurrent net …
The spontaneous synaptic plasticity depended on a rise in intracellular calcium concentrations of postsynaptic cells, but not on NMDA …
Watching neuronal circuit dynamics through functional multineuron calcium imaging (fMCI).
Takahashi N, Sasaki T, Usami A, Matsuki N, Ikegaya Y. Takahashi N, et al. Among authors: usami a. Neurosci Res. 2007 Jul;58(3):219-25. doi: 10.1016/j.neures.2007.03.001. Epub 2007 Mar 13. Neurosci Res. 2007. PMID: 17418439 Review.
Functional multineuron calcium imaging (fMCI) is a large-scale optical recording technique that monitors the spatiotemporal pattern of action potentials, all at once, from large neuron populations. fMCI has unique advantages, including: (i) simultaneous recording from > …
Functional multineuron calcium imaging (fMCI) is a large-scale optical recording technique that monitors the spatiotemporal pattern o …
A low-cost method for brain slice cultures.
Koyama R, Muramatsu R, Sasaki T, Kimura R, Ueyama C, Tamura M, Tamura N, Ichikawa J, Takahashi N, Usami A, Yamada MK, Matsuki N, Ikegaya Y. Koyama R, et al. Among authors: usami a. J Pharmacol Sci. 2007 Jun;104(2):191-4. doi: 10.1254/jphs.sc0070119. Epub 2007 Jun 8. J Pharmacol Sci. 2007. PMID: 17558179
We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistingu …
We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost red …
The effect of pH, hydrogen peroxide and temperature on the stability of human monoclonal antibody.
Usami A, Ohtsu A, Takahama S, Fujii T. Usami A, et al. J Pharm Biomed Anal. 1996 Jun;14(8-10):1133-40. doi: 10.1016/s0731-7085(96)01721-9. J Pharm Biomed Anal. 1996. PMID: 8818025
The stability of human monoclonal antibody (C23), which is being developed as a passive immunotherapeutic agent against human cytomegalovirus, was investigated. ...
The stability of human monoclonal antibody (C23), which is being developed as a passive immunotherapeutic agent against human cytomeg …
Japanese Guideline for Occupational Allergic Diseases 2014.
Dobashi K, Akiyama K, Usami A, Yokozeki H, Ikezawa Z, Tsurikisawa N, Nakamura Y, Sato K, Okumura J; Committee for Japanese Guideline for Diagnosis; Management of Occupational Allergic Diseases; Japanese Society of Allergology. Dobashi K, et al. Among authors: usami a. Allergol Int. 2014;63(3):421-442. doi: 10.2332/allergolint.14-RAI-0771. Epub 2015 Feb 27. Allergol Int. 2014. PMID: 28942932 Review.
In 2013, a guideline for occupational allergic diseases was published for the first time in Japan. Occupational allergic diseases are likely to worsen or become intractable as a result of continuous exposure to high concentrations of causative antigens, and are soci …
In 2013, a guideline for occupational allergic diseases was published for the first time in Japan. Occupational allergic diseases are …
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