Abstract
alpha-Subunit of the Torpedo californica nicotinic acetylcholine receptor was isolated by preparative SDS-PAGE followed by reversed-phase HPLC on a C4 column in an acetonitrile-isopropanol gradient in water. After removal of the organic solvents and solubilization in beta-octylglucoside, the purified alpha-subunit binds alpha-bungarotoxin with high affinity (Kd 28 nM).
Publication types
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English Abstract
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Letter
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bungarotoxins / chemistry
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Bungarotoxins / metabolism*
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Chromatography, High Pressure Liquid
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Electrophoresis, Polyacrylamide Gel
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Receptors, Nicotinic / chemistry
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Receptors, Nicotinic / isolation & purification
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Receptors, Nicotinic / metabolism*
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Solubility
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Torpedo
Substances
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Bungarotoxins
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Receptors, Nicotinic