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Highly active BRET-reporter based on yellow mutant of Renilla muelleri luciferase.
Eremeeva EV, Markova SV, Vysotski ES. Eremeeva EV, et al. Dokl Biochem Biophys. 2013 May-Jun;450:147-50. doi: 10.1134/S1607672913030095. Epub 2013 Jul 4. Dokl Biochem Biophys. 2013. PMID: 23824457 No abstract available.
Mn(2+)-activated luminescence of the photoprotein obelin.
Vysotski ES, Trofimov CP, Bondaƕ VS, Frank LA, Markova SV, Illarionov BA. Vysotski ES, et al. Arch Biochem Biophys. 1995 Jan 10;316(1):92-9. doi: 10.1006/abbi.1995.1014. Arch Biochem Biophys. 1995. PMID: 7840683
Cotranslational formation of active photoprotein obelin in a cell-free translation system: direct ultrahigh sensitive measure of the translation course.
Berestovskaya NG, Shaloiko LA, Gorokhovatsky AY, Bondar VS, Vysotski ES, Maximov JE, von Doehren H, Alakhov YB. Berestovskaya NG, et al. Anal Biochem. 1999 Mar 1;268(1):72-8. doi: 10.1006/abio.1998.3051. Anal Biochem. 1999. PMID: 10036164
Obelin from the bioluminescent marine hydroid Obelia geniculata: cloning, expression, and comparison of some properties with those of other Ca2+-regulated photoproteins.
Markova SV, Vysotski ES, Blinks JR, Burakova LP, Wang BC, Lee J. Markova SV, et al. Biochemistry. 2002 Feb 19;41(7):2227-36. doi: 10.1021/bi0117910. Biochemistry. 2002. PMID: 11841214
Cloning and expression of cDNA for a luciferase from the marine copepod Metridia longa. A novel secreted bioluminescent reporter enzyme.
Markova SV, Golz S, Frank LA, Kalthof B, Vysotski ES. Markova SV, et al. J Biol Chem. 2004 Jan 30;279(5):3212-7. doi: 10.1074/jbc.M309639200. Epub 2003 Oct 28. J Biol Chem. 2004. PMID: 14583604
Bioluminescent immunoassay of thyrotropin and thyroxine using obelin as a label.
Frank LA, Petunin AI, Vysotski ES. Frank LA, et al. Anal Biochem. 2004 Feb 15;325(2):240-6. doi: 10.1016/j.ab.2003.11.003. Anal Biochem. 2004. PMID: 14751258
Interchange of aequorin and obelin bioluminescence color is determined by substitution of one active site residue of each photoprotein.
Stepanyuk GA, Golz S, Markova SV, Frank LA, Lee J, Vysotski ES. Stepanyuk GA, et al. FEBS Lett. 2005 Feb 14;579(5):1008-14. doi: 10.1016/j.febslet.2005.01.004. Epub 2005 Jan 13. FEBS Lett. 2005. PMID: 15710383
Coelenterazine-binding protein of Renilla muelleri: cDNA cloning, overexpression, and characterization as a substrate of luciferase.
Titushin MS, Markova SV, Frank LA, Malikova NP, Stepanyuk GA, Lee J, Vysotski ES. Titushin MS, et al. Photochem Photobiol Sci. 2008 Feb;7(2):189-96. doi: 10.1039/b713109g. Epub 2007 Dec 10. Photochem Photobiol Sci. 2008. PMID: 18264586
Violet and greenish photoprotein obelin mutants for reporter applications in dual-color assay.
Frank LA, Borisova VV, Markova SV, Malikova NP, Stepanyuk GA, Vysotski ES. Frank LA, et al. Anal Bioanal Chem. 2008 Aug;391(8):2891-6. doi: 10.1007/s00216-008-2223-5. Epub 2008 Jul 5. Anal Bioanal Chem. 2008. PMID: 18604525
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