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Opportunities for improved serodiagnosis of human tuberculosis, bovine tuberculosis, and paratuberculosis.
Wadhwa A, Hickling GJ, Eda S. Wadhwa A, et al. Vet Med Int. 2012;2012:674238. doi: 10.1155/2012/674238. Epub 2012 Jun 6. Vet Med Int. 2012. PMID: 22720192 Free PMC article.
A review of recent attempts to improve diagnostic test performance indicates that the use of multiple antigens can improve the accuracy of serodiagnosis of these mycobacterial diseases. Mycobacteria also produce a variety of species-specific nonprotein molecules; ho
A review of recent attempts to improve diagnostic test performance indicates that the use of multiple antigens can improve the accura
Structure determination of lipopeptides from Mycobacterium avium subspecies paratuberculosis and identification of antigenic lipopeptide probes.
Mitachi K, Sharma Gautam LN, Rice JH, Eda K, Wadhwa A, Momotani E, Hlopak JP, Eda S, Kurosu M. Mitachi K, et al. Anal Biochem. 2016 Jul 15;505:29-35. doi: 10.1016/j.ab.2016.04.001. Epub 2016 Apr 22. Anal Biochem. 2016. PMID: 27114041 Free PMC article.
MAP infection of intestinal tissue triggers a fatal inflammatory disorder, Johne's disease (paratuberculosis). Development of fast and reliable diagnostic methods for Johne's disease in clinically suspected ruminants requires the discovery of MAP-specific antigens that ind …
MAP infection of intestinal tissue triggers a fatal inflammatory disorder, Johne's disease (paratuberculosis). Development of fast an …
Evaluation of ethanol vortex ELISA for detection of bovine tuberculosis in cattle and deer.
Wadhwa A, Johonson RE, Eda K, Waters WR, Palmer MV, Bannantine JP, Eda S. Wadhwa A, et al. BMC Vet Res. 2014 Jul 4;10:147. doi: 10.1186/1746-6148-10-147. BMC Vet Res. 2014. PMID: 24992970 Free PMC article.
Use of ethanol extract of Mycobacterium bovis for detection of specific antibodies in sera of farmed red deer (Cervus elaphus) with bovine tuberculosis.
Wadhwa A, Johnson RE, Mackintosh CG, Griffin JF, Waters WR, Bannantine JP, Eda S. Wadhwa A, et al. BMC Vet Res. 2013 Dec 17;9:256. doi: 10.1186/1746-6148-9-256. BMC Vet Res. 2013. PMID: 24341485 Free PMC article.
We tested a total of 45 red deer serum samples, divided in 3 groups - uninfected animals (n = 15), experimentally infected with M. bovis (n = 15) and experimentally infected with MAP (n = 15). ...CONCLUSIONS: The results from this study suggest that EVELISA may form a
We tested a total of 45 red deer serum samples, divided in 3 groups - uninfected animals (n = 15), experimentally infected with M. bo …
An AC electrokinetic impedance immunosensor for rapid detection of tuberculosis.
Cui H, Li S, Yuan Q, Wadhwa A, Eda S, Chambers M, Ashford R, Jiang H, Wu J. Cui H, et al. Analyst. 2013 Dec 7;138(23):7188-96. doi: 10.1039/c3an01112g. Analyst. 2013. PMID: 24135732
The results were consistent with those obtained by a conventional ELISA method. The limit of detection of the impedance sensing method was estimated to be better than 10 ng mL(-1). ...This method may form a basis for development of a point of care diagnostic …
The results were consistent with those obtained by a conventional ELISA method. The limit of detection of the impedance sensing metho …
AC electrokinetics-enhanced capacitive immunosensor for point-of-care serodiagnosis of infectious diseases.
Li S, Cui H, Yuan Q, Wu J, Wadhwa A, Eda S, Jiang H. Li S, et al. Biosens Bioelectron. 2014 Jan 15;51:437-43. doi: 10.1016/j.bios.2013.08.016. Epub 2013 Aug 23. Biosens Bioelectron. 2014. PMID: 24007749
The capacitive immunosensor presented here employs elevated AC potentials at a fixed frequency for impedimetric interrogation of the microelectrodes. ...The ACEK-enhanced capacitive immunosensor is a platform technology, and can be employed to detect any combination …
The capacitive immunosensor presented here employs elevated AC potentials at a fixed frequency for impedimetric interrogation of the …
Optimization of serum EVELISA for milk testing of Johne's disease.
Wadhwa A, Bannantine JP, Byrem TM, Stein TL, Saxton AM, Speer CA, Eda S. Wadhwa A, et al. Foodborne Pathog Dis. 2012 Aug;9(8):749-54. doi: 10.1089/fpd.2011.1115. Epub 2012 Jul 30. Foodborne Pathog Dis. 2012. PMID: 22845627
In our previous study, we developed a sensitive serum ELISA test, ethanol-vortex enzyme-linked immunosorbent assay (EVELISA), using ethanol extract of MAP. ...Using the optimized conditions, a total of 57 milk samples from Holstein dairy cattle (37 animals found pos …
In our previous study, we developed a sensitive serum ELISA test, ethanol-vortex enzyme-linked immunosorbent assay (EVELISA), using e …
Bead-based microfluidic immunoassay for diagnosis of Johne's disease.
Wadhwa A, Foote RS, Shaw RW, Eda S. Wadhwa A, et al. J Immunol Methods. 2012 Aug 31;382(1-2):196-202. doi: 10.1016/j.jim.2012.06.006. Epub 2012 Jun 15. J Immunol Methods. 2012. PMID: 22705087
Microfluidic assays were performed by magnetically immobilizing a number of beads within a microchannel of a glass microchip and detecting antibody on the collected beads using laser-induced fluorescence. ...In a further experiment, we magnetically imm …
Microfluidic assays were performed by magnetically immobilizing a number of beads within a microchannel of a glass micr …
Assessment of the immunogenicity of rabies vaccine preserved by vaporization and delivered to the duodenal mucosa of gray foxes (Urocyon cinereoargenteus).
Smith TG, Wu X, Ellison JA, Wadhwa A, Franka R, Langham GL, Skinner BL, Hanlon CA, Bronshtein VL. Smith TG, et al. Am J Vet Res. 2017 Jun;78(6):752-756. doi: 10.2460/ajvr.78.6.752. Am J Vet Res. 2017. PMID: 28541146 Free PMC article. Clinical Trial.
Saliva samples were collected weekly and tested for virus shedding by use of a conventional reverse-transcriptase PCR assay. ...A safe, potent, thermostable RABV vaccine that could be delivered orally to wildlife or domestic animals would enhance current rabies cont …
Saliva samples were collected weekly and tested for virus shedding by use of a conventional reverse-transcriptase PCR assay. ...A
Rabies surveillance in the United States during 2014.
Monroe BP, Yager P, Blanton J, Birhane MG, Wadhwa A, Orciari L, Petersen B, Wallace R. Monroe BP, et al. J Am Vet Med Assoc. 2016 Apr 1;248(7):777-88. doi: 10.2460/javma.248.7.777. J Am Vet Med Assoc. 2016. PMID: 27003019 No abstract available.
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