Wan Y - Search Results

1

Calcium chloride and arginine show diametrically opposite effects on antibody elution in Protein A and Protein L chromatography.

Qu J, Wan Y, Li Y. Qu J, et al. Among authors: wan y. J Biol Methods. 2024 Jul 10;11(2):e99010016. doi: 10.14440/jbm.2024.0006. eCollection 2024. J Biol Methods. 2024. PMID: 39323484 Free PMC article.

2

Sodium caprylate induced precipitation post Protein A chromatography as an effective means for host cell protein clearance.

Wan Y, Zhang T, Chen T, Wang Y, Li Y. Wan Y, et al. Protein Expr Purif. 2019 Dec;164:105460. doi: 10.1016/j.pep.2019.105460. Epub 2019 Jul 25. Protein Expr Purif. 2019. PMID: 31351123

3

Comparing the relative robustness of byproduct removal by wash and by elution in column chromatography for the purification of a bispecific antibody.

Wan Y, Zhang T, Wang Y, Li Y. Wan Y, et al. Protein Expr Purif. 2021 Jan;177:105762. doi: 10.1016/j.pep.2020.105762. Epub 2020 Sep 21. Protein Expr Purif. 2021. PMID: 32971297

4

Application of pH-salt dual gradient elution in purifying a WuXiBody-based bispecific antibody by MMC ImpRes mixed-mode chromatography.

Wan Y, Wang Y, Zhang T, Zhang S, Wang Y, Li Y. Wan Y, et al. Protein Expr Purif. 2021 May;181:105822. doi: 10.1016/j.pep.2021.105822. Epub 2021 Jan 9. Protein Expr Purif. 2021. PMID: 33429037

5

Removing a single-arm species by Fibro PrismA in purifying an asymmetric IgG-like bispecific antibody.

Zhang T, Wan Y, Wang Y, Li Y. Zhang T, et al. Among authors: wan y. Protein Expr Purif. 2021 Jun;182:105847. doi: 10.1016/j.pep.2021.105847. Epub 2021 Feb 13. Protein Expr Purif. 2021. PMID: 33588033

6

Comparing the relative robustness of anion exchange and the corresponding mixed-mode chromatography on removing a weakly-bound byproduct: A case study of bispecific antibody purification.

Zhang S, Zhang T, Li Y, Wan Y. Zhang S, et al. Among authors: wan y. Protein Expr Purif. 2021 Nov;187:105948. doi: 10.1016/j.pep.2021.105948. Epub 2021 Jul 22. Protein Expr Purif. 2021. PMID: 34303809

7

Assessing four subdomain-specific affinity resins' capability to separate half-antibody from intact bispecific antibody.

Chen W, Zhang T, Wan Y, Li Y. Chen W, et al. Among authors: wan y. Protein Expr Purif. 2022 Oct;198:106124. doi: 10.1016/j.pep.2022.106124. Epub 2022 May 31. Protein Expr Purif. 2022. PMID: 35661701

8

Acidic pH or salt treatment can convert soluble antibody aggregates in culture harvest into monomers and improve Protein A chromatography step yield.

Zhang T, Li B, Dong W, Wan Y, Li Y. Zhang T, et al. Among authors: wan y. Protein Expr Purif. 2024 Mar;215:106391. doi: 10.1016/j.pep.2023.106391. Epub 2023 Nov 7. Protein Expr Purif. 2024. PMID: 37939750

9

Different VH3-binding Protein A Resins Show Comparable VH3-binding Mediated Byproduct Separation Capabilities Despite Having Varied Dynamic Binding Capacities Towards A VH3 Fab.

Hu L, Wang R, Wu Q, Wan Y, Li Y. Hu L, et al. Among authors: wan y. Protein Pept Lett. 2024;31(8):611-618. doi: 10.2174/0109298665320125240805112024. Protein Pept Lett. 2024. PMID: 39161140

10

Partition coefficient screening - An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case.

Chen W, Zhang T, Wang PK, Liao CC, Li Y, Wan Y. Chen W, et al. Among authors: wan y. Protein Expr Purif. 2025 Jan;225:106583. doi: 10.1016/j.pep.2024.106583. Epub 2024 Aug 20. Protein Expr Purif. 2025. PMID: 39168394

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