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[Inducing hepatocellular carcinoma-specific cytotoxic T lymphocyte response using formed by fusion of FastDCs and allogeneic human hepatocellular carcinoma cells].
Guan X, Leng XS, Peng JR, Wei YH, Wang H, Yuan L. Guan X, et al. Among authors: Wang H. Zhonghua Yi Xue Za Zhi. 2005 Dec 14;85(47):3332-6. Zhonghua Yi Xue Za Zhi. 2005. PMID: 16409838 Chinese.
METHODS: CD14(+) cells were isolated and purified from the peripheral blood of a healthy HLA-A2 blood donor and cultured in fresh dendritic cell complete medium for 24 h, then proinflammatory mediators were supplemented for another 24 h, thus generating mature dendritic cell (FastDCs). ...T cells were isolated from selected CD14(-) cells and then divided into 4 groups to be stimulated with dendritoma cells, DCs, HCCLM3 cells, and mixed DCs-HCCLM3 cells respectively for 96 hours. 18 hours before the end of cultivation (3)H-TdR was added into the culture fluid. ...
METHODS: CD14(+) cells were isolated and purified from the peripheral blood of a healthy HLA-A2 blood donor and cultured in fresh dendritic …
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