Oil degradation and biosurfactant production by the deep sea bacterium Dietzia maris As-13-3

Wanpeng Wang; Bobo Cai; Zongze Shao

doi:10.3389/fmicb.2014.00711

Oil degradation and biosurfactant production by the deep sea bacterium Dietzia maris As-13-3

Front Microbiol. 2014 Dec 16:5:711. doi: 10.3389/fmicb.2014.00711. eCollection 2014.

Authors

Wanpeng Wang¹, Bobo Cai², Zongze Shao¹

Affiliations

¹ Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration Xiamen, China ; State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration Xiamen, China ; Collaborative Innovation Center of Deep Sea Biology, Third Institute of Oceanography, State Oceanic Administration Xiamen, China ; Key Laboratory of Marine Genetic Resources of Fujian Province Xiamen, China ; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources Xiamen, China.
² Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration Xiamen, China ; State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration Xiamen, China ; Collaborative Innovation Center of Deep Sea Biology, Third Institute of Oceanography, State Oceanic Administration Xiamen, China ; Key Laboratory of Marine Genetic Resources of Fujian Province Xiamen, China ; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources Xiamen, China ; Life Science College, Xiamen University Xiamen, China.

Abstract

Recent investigations of extreme environments have revealed numerous bioactive natural products. However, biosurfactant-producing strains from deep sea extreme environment are largely unknown. Here, we show that Dietzia maris As-13-3 isolated from deep sea hydrothermal field could produce di-rhamnolipid as biosurfactant. The critical micelle concentration (CMC) of the purified di-rhamnolipid was determined to be 120 mgL(-1), and it lowered the surface tension of water from 74 ± 0.2 to 38 ± 0.2 mN m(-1). Further, the alkane metabolic pathway-related genes and di-rhamnolipid biosynthesis-related genes were also analyzed by the sequencing genome of D. maris As-13-3 and quantitative real-time PCR (Q-PCR), respectively. Q-PCR analysis showed that all these genes were induced by n-Tetradecane, n-Hexadecane, and pristane. To the best of our knowledge, this is first report about the complete pathway of the di-rhamnolipid synthesis process in the genus Dietzia. Thus, our study provided the insights into Dietzia in respects of oil degradation and biosurfactant production, and will help to evaluate the potential of Dietzia in marine oil removal.

Keywords: Dietzia; biosurfactant; biosynthesis pathway; di-rhamnolipid; genome sequence; hydrocarbon degradation.