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Techniques used in studies of age-related DNA methylation changes.
Wojdacz TK, Hansen LL. Wojdacz TK, et al. Ann N Y Acad Sci. 2006 May;1067:479-87. doi: 10.1196/annals.1354.069. Ann N Y Acad Sci. 2006. PMID: 16804030 Review.
Melting curve assays for DNA methylation analysis.
Wojdacz TK, Dobrovic A. Wojdacz TK, et al. Methods Mol Biol. 2009;507:229-40. doi: 10.1007/978-1-59745-522-0_17. Methods Mol Biol. 2009. PMID: 18987818
Methylation-sensitive high-resolution melting.
Wojdacz TK, Dobrovic A, Hansen LL. Wojdacz TK, et al. Nat Protoc. 2008;3(12):1903-8. doi: 10.1038/nprot.2008.191. Nat Protoc. 2008. PMID: 19180074
Primer design versus PCR bias in methylation independent PCR amplifications.
Wojdacz TK, Borgbo T, Hansen LL. Wojdacz TK, et al. Epigenetics. 2009 May 16;4(4):231-4. doi: 10.4161/epi.9020. Epub 2009 May 14. Epigenetics. 2009. PMID: 19483476
Quality assessment of DNA derived from up to 30 years old formalin fixed paraffin embedded (FFPE) tissue for PCR-based methylation analysis using SMART-MSP and MS-HRM.
Kristensen LS, Wojdacz TK, Thestrup BB, Wiuf C, Hager H, Hansen LL. Kristensen LS, et al. BMC Cancer. 2009 Dec 21;9:453. doi: 10.1186/1471-2407-9-453. BMC Cancer. 2009. PMID: 20025721 Free PMC article.
Limitations and advantages of MS-HRM and bisulfite sequencing for single locus methylation studies.
Wojdacz TK, Møller TH, Thestrup BB, Kristensen LS, Hansen LL. Wojdacz TK, et al. Expert Rev Mol Diagn. 2010 Jul;10(5):575-80. doi: 10.1586/erm.10.46. Expert Rev Mol Diagn. 2010. PMID: 20629507 Review.
The methylation-sensitive high-resolution melting (MS-HRM) protocol, as described by Wojdacz and Dobrovic, enables detection of a methylated template in an unmethylated background, with sensitivity similar to that of methylation-specific PCR (MSP). ...
The methylation-sensitive high-resolution melting (MS-HRM) protocol, as described by Wojdacz and Dobrovic, enables detection of a met …
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