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Structure of the core domain of human cardiac troponin in the Ca(2+)-saturated form.
Takeda S, Yamashita A, Maeda K, Maéda Y. Takeda S, et al. Nature. 2003 Jul 3;424(6944):35-41. doi: 10.1038/nature01780. Nature. 2003. PMID: 12840750
The alpha-helical coiled-coil formed between TnT and TnI is integrated in a rigid and asymmetric structure (about 80 angstrom long), the IT arm, which bridges putative tropomyosin-anchoring regions. ...
The alpha-helical coiled-coil formed between TnT and TnI is integrated in a rigid and asymmetric structure (about 80 angstrom long), …
Molecular bases of multimodal regulation of a fungal transient receptor potential (TRP) channel.
Ihara M, Hamamoto S, Miyanoiri Y, Takeda M, Kainosho M, Yabe I, Uozumi N, Yamashita A. Ihara M, et al. J Biol Chem. 2013 May 24;288(21):15303-17. doi: 10.1074/jbc.M112.434795. Epub 2013 Apr 3. J Biol Chem. 2013. PMID: 23553631 Free PMC article.
TRPGz possesses a cytosolic C-terminal domain (CTD), primarily composed of intrinsically disordered regions with some regulatory modules, a putative coiled-coil region and a basic residue cluster. ...The crystal structure of the presumed coiled-coil region re …
TRPGz possesses a cytosolic C-terminal domain (CTD), primarily composed of intrinsically disordered regions with some regulatory modu …
Capturing enzyme structure prior to reaction initiation: tropinone reductase-II-substrate complexes.
Yamashita A, Endo M, Higashi T, Nakatsu T, Yamada Y, Oda J, Kato H. Yamashita A, et al. Biochemistry. 2003 May 20;42(19):5566-73. doi: 10.1021/bi0272712. Biochemistry. 2003. PMID: 12741812
A combination of two kinetic crystallographic techniques, a continuous flow of the substrates and Laue diffraction measurements, enabled us to capture the transit structures prior to the reaction proceeding. A structure comparison of the enzyme-substrate comp
A combination of two kinetic crystallographic techniques, a continuous flow of the substrates and Laue diffraction measurement
Crystal structure of the C-terminal half of tropomodulin and structural basis of actin filament pointed-end capping.
Krieger I, Kostyukova A, Yamashita A, Nitanai Y, Maéda Y. Krieger I, et al. Biophys J. 2002 Nov;83(5):2716-25. doi: 10.1016/S0006-3495(02)75281-8. Biophys J. 2002. PMID: 12414704 Free PMC article.
C20 is a leucine-rich repeat domain, and this is the first actin-associated protein with a leucine-rich repeat. Binding assays suggested that C20 also interacts with the N-terminal fragment, M1-M2-M3, of nebulin. Based on the crystal structure, we propose a m …
C20 is a leucine-rich repeat domain, and this is the first actin-associated protein with a leucine-rich repeat. Binding assays …
Crystal structure of CapZ: structural basis for actin filament barbed end capping.
Yamashita A, Maeda K, Maéda Y. Yamashita A, et al. EMBO J. 2003 Apr 1;22(7):1529-38. doi: 10.1093/emboj/cdg167. EMBO J. 2003. PMID: 12660160 Free PMC article.
Capping protein, a heterodimeric protein composed of alpha and beta subunits, is a key cellular component regulating actin filament assembly and organization. ...Here we describe the crystal structure of the chicken sarcomeric capping protein CapZ at 2.1 A re …
Capping protein, a heterodimeric protein composed of alpha and beta subunits, is a key cellular component regulating actin fil …
Structural basis for perception of diverse chemical substances by T1r taste receptors.
Nuemket N, Yasui N, Kusakabe Y, Nomura Y, Atsumi N, Akiyama S, Nango E, Kato Y, Kaneko MK, Takagi J, Hosotani M, Yamashita A. Nuemket N, et al. Nat Commun. 2017 May 23;8:15530. doi: 10.1038/ncomms15530. Nat Commun. 2017. PMID: 28534491 Free PMC article.
These receptors function as T1r2-T1r3 and T1r1-T1r3 heterodimers to recognize a wide array of sweet and umami (savory) tastes in sugars and amino acids. ...Overall, we provide a structural basis for understanding the chemical perception of taste receptors....
These receptors function as T1r2-T1r3 and T1r1-T1r3 heterodimers to recognize a wide array of sweet and umami (savory) tastes in suga …
Crystal structures of Lymnaea stagnalis AChBP in complex with neonicotinoid insecticides imidacloprid and clothianidin.
Ihara M, Okajima T, Yamashita A, Oda T, Hirata K, Nishiwaki H, Morimoto T, Akamatsu M, Ashikawa Y, Kuroda S, Mega R, Kuramitsu S, Sattelle DB, Matsuda K. Ihara M, et al. Invert Neurosci. 2008 Jun;8(2):71-81. doi: 10.1007/s10158-008-0069-3. Epub 2008 Mar 13. Invert Neurosci. 2008. PMID: 18338186 Free PMC article.
The crystal structures suggested that the guanidine moiety of IMI and CTD stacks with Tyr185, while the nitro group of IMI but not of CTD makes a hydrogen bond with Gln55. ...Yet, the NH at position 1 of CTD makes a hydrogen bond with the backbone carbonyl of Trp143 …
The crystal structures suggested that the guanidine moiety of IMI and CTD stacks with Tyr185, while the nitro group of IMI but not of CTD ma …
Distinct human and mouse membrane trafficking systems for sweet taste receptors T1r2 and T1r3.
Shimizu M, Goto M, Kawai T, Yamashita A, Kusakabe Y. Shimizu M, et al. PLoS One. 2014 Jul 16;9(7):e100425. doi: 10.1371/journal.pone.0100425. eCollection 2014. PLoS One. 2014. PMID: 25029362 Free PMC article.
A domain-swapped chimera and truncated human T1R3 mutant showed that the Venus flytrap module and cysteine-rich domain (CRD) of human T1R3 contain a region related to the inhibition of human T1R3 membrane trafficking and coordinated regulation of human T1R3 membrane
A domain-swapped chimera and truncated human T1R3 mutant showed that the Venus flytrap module and cysteine-rich domain (CRD) of human
A large-scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand-binding domains of taste receptor.
Yamashita A, Nango E, Ashikawa Y. Yamashita A, et al. Protein Sci. 2017 Nov;26(11):2291-2301. doi: 10.1002/pro.3271. Epub 2017 Sep 6. Protein Sci. 2017. PMID: 28833672 Free PMC article.
Here we report a strategy addressing large-scale expression of hetero-multimeric extracellular domains of plasma membrane proteins and its application to the extracellular domains of a taste receptor. ...We achieved the successful preparation of the heterodimeric LB …
Here we report a strategy addressing large-scale expression of hetero-multimeric extracellular domains of plasma membrane proteins an …
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