An iridium complex-based probe for photoluminescence lifetime imaging of human carboxylesterase 2 in living cells

Zihe Yan; Jinyu Wang; Yanxin Zhang; Sichun Zhang; Juan Qiao; Xinrong Zhang

doi:10.1039/c8cc04481c

An iridium complex-based probe for photoluminescence lifetime imaging of human carboxylesterase 2 in living cells

Chem Commun (Camb). 2018 Aug 21;54(65):9027-9030. doi: 10.1039/c8cc04481c. Epub 2018 Jul 26.

Authors

Zihe Yan¹, Jinyu Wang¹, Yanxin Zhang², Sichun Zhang¹, Juan Qiao², Xinrong Zhang¹

Affiliations

¹ Department of Chemistry, Beijing Key laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China. xrzhang@mail.tsinghua.edu.cn.
² Department of Chemistry, Key Lab of Organic Optoelectronics and Molecular Engineering of Ministry of Education, Tsinghua University, Beijing 100084, China. qjuan@mail.tsinghua.edu.cn.

PMID: 30047956
DOI: 10.1039/c8cc04481c

Abstract

A novel photoluminescence lifetime probe (Ir-TB) has been developed for the detection and imaging of hCE2 in living cells. A large lifetime increase by around 300 ns after the enzymatic reaction makes it an ideal tool to distinguish hCE2-hydrolyzed probes from those non-hydrolyzed ones via PLIM for the first time.

MeSH terms

Carboxylesterase / analysis*
Carboxylesterase / metabolism
Coordination Complexes / chemistry
Coordination Complexes / pharmacology*
Hep G2 Cells
Humans
Hydrolysis
Iridium / chemistry*
Kinetics
Light
Luminescence
Luminescent Agents / chemistry
Luminescent Agents / pharmacology*
Models, Chemical
Quantum Theory

Substances

Coordination Complexes
Luminescent Agents
Iridium
CES2 protein, human
Carboxylesterase