Development of a quantitative relationship between inhibition percentage and both incubation time and inhibitor concentration for inhibition biosensors--theoretical and practical considerations

Biosens Bioelectron. 2001 Dec;16(9-12):1119-26. doi: 10.1016/s0956-5663(01)00240-8.

Abstract

Theoretical and practical insights into the design and development of immobilised enzyme inhibition biosensors are reported. A general mathematical expression relating the percent of enzyme inhibition (i.e. the analytical signal) to both the inhibitor concentration and the incubation time is presented. The relevant physical, chemical and biochemical parameters required by the model are developed and discussed in terms of the inhibition of acetylcholinesterase by the organophosphorous pesticide, paraoxon. A second enzyme, choline oxidase and an amperometric transducer are used to facilitate the determination acetylcholinesterase inhibitor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Acetylcholinesterase
  • Alcohol Oxidoreductases
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Biosensing Techniques / statistics & numerical data
  • Cholinesterase Inhibitors / analysis
  • Electrochemistry
  • Environmental Pollutants / analysis
  • Enzymes, Immobilized
  • Equipment Design
  • Hydrogen-Ion Concentration
  • Insecticides / analysis
  • Models, Theoretical
  • Paraoxon / analysis
  • Transducers

Substances

  • Cholinesterase Inhibitors
  • Environmental Pollutants
  • Enzymes, Immobilized
  • Insecticides
  • Alcohol Oxidoreductases
  • choline oxidase
  • Acetylcholinesterase
  • Paraoxon