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Page 1
Search for a crosslinker-based identification instead (1 results)
Protein-protein interactions identified by pull-down experiments and mass spectrometry.
Brymora A, Valova VA, Robinson PJ. Brymora A, et al. Curr Protoc Cell Biol. 2004 May;Chapter 17:Unit 17.5. doi: 10.1002/0471143030.cb1705s22. Curr Protoc Cell Biol. 2004. PMID: 18228443 Review.
The aim of this unit is to provide a method for the identification of new protein-protein interactions. Pull-down experiments with GST fusion proteins attached to glutathione beads are a screening technique for identification of protein-protein interactions. ...Appr …
The aim of this unit is to provide a method for the identification of new protein-protein interactions. Pull-down experiments with GS …
A crosslinker-based identification of redox relay targets.
Araki K, Ushioda R, Kusano H, Tanaka R, Hatta T, Fukui K, Nagata K, Natsume T. Araki K, et al. Anal Biochem. 2017 Mar 1;520:22-26. doi: 10.1016/j.ab.2016.12.025. Epub 2016 Dec 31. Anal Biochem. 2017. PMID: 28048978
Thiol-based redox control is among the most important mechanisms for maintaining cellular redox homeostasis, with essential participation of cysteine thiols of oxidoreductases. ...We applied a recently described thiol-ene crosslinking-based strategy, named di …
Thiol-based redox control is among the most important mechanisms for maintaining cellular redox homeostasis, with essential participa …
Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP.
Hafner M, Landthaler M, Burger L, Khorshid M, Hausser J, Berninger P, Rothballer A, Ascano M Jr, Jungkamp AC, Munschauer M, Ulrich A, Wardle GS, Dewell S, Zavolan M, Tuschl T. Hafner M, et al. Cell. 2010 Apr 2;141(1):129-41. doi: 10.1016/j.cell.2010.03.009. Cell. 2010. PMID: 20371350 Free PMC article.
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking appro …
RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing …
Identification of IgE.
Ishizaka K, Ishizaka T. Ishizaka K, et al. J Allergy Clin Immunol. 2016 Jun;137(6):1646-1650. doi: 10.1016/j.jaci.2015.12.1343. Epub 2016 Apr 15. J Allergy Clin Immunol. 2016. PMID: 27090936 Free article. Review.
Our studies were based on the hypothesis that reaginic antibody activity is associated with a unique immunoglobulin isotype, which is now called IgE. As expected, identification of IgE facilitated the analysis of immunologic mechanisms of reaginic hypersensitivity. …
Our studies were based on the hypothesis that reaginic antibody activity is associated with a unique immunoglobulin isotype, which is …
A mechanism-based cross-linker for the identification of kinase-substrate pairs.
Maly DJ, Allen JA, Shokat KM. Maly DJ, et al. J Am Chem Soc. 2004 Aug 4;126(30):9160-1. doi: 10.1021/ja048659i. J Am Chem Soc. 2004. PMID: 15281787
Recently, there has been substantial progress made in the identification of new phosphoproteins and phosphorylation sites. Unfortunately, there are very few methods available that allow this information to be used to identify the upstream kinase responsible for the phospho …
Recently, there has been substantial progress made in the identification of new phosphoproteins and phosphorylation sites. Unfortunat …
Elastin.
Mithieux SM, Weiss AS. Mithieux SM, et al. Adv Protein Chem. 2005;70:437-61. doi: 10.1016/S0065-3233(05)70013-9. Adv Protein Chem. 2005. PMID: 15837523 Review.
Tropoelastin associates with multiple tropoelastin molecules during the major phase of elastogenesis through coacervation, where this process is directed by the precise patterning of mostly alternating hydrophobic and hydrophilic sequences that dictate intermolecular alignment. M …
Tropoelastin associates with multiple tropoelastin molecules during the major phase of elastogenesis through coacervation, where this proces …
A Glycosidic-Bond-Based Mass-Spectrometry-Cleavable Cross-linker Enables In Vivo Cross-linking for Protein Complex Analysis.
Chen J, Zhao Q, Gao H, Zhao L, Chu H, Shan Y, Liang Z, Zhang Y, Zhang L. Chen J, et al. Angew Chem Int Ed Engl. 2023 Jun 12;62(24):e202212860. doi: 10.1002/anie.202212860. Epub 2023 May 4. Angew Chem Int Ed Engl. 2023. PMID: 36998115
However, the progress of in vivo CXMS studies has been limited by cross-linking biocompatibility and data analysis. Herein, a glycosidic bond-based MS-cleavable cross-linker of trehalose disuccinimidyl ester (TDS) was designed and synthesized, which was fragmented in MS un …
However, the progress of in vivo CXMS studies has been limited by cross-linking biocompatibility and data analysis. Herein, a glycosidic bon …
Target identification of small molecules based on chemical biology approaches.
Futamura Y, Muroi M, Osada H. Futamura Y, et al. Mol Biosyst. 2013 May;9(5):897-914. doi: 10.1039/c2mb25468a. Mol Biosyst. 2013. PMID: 23354001 Review.
Recently, a phenotypic approach-screens that assess the effects of compounds on cells, tissues, or whole organisms-has been reconsidered and reintroduced as a complementary strategy of a target-based approach for drug discovery. Although the finding of novel bioactive comp …
Recently, a phenotypic approach-screens that assess the effects of compounds on cells, tissues, or whole organisms-has been reconsidered and …
Affinity-based protein profiling-driven discovery of myricanol as a Nampt activator.
Lyu P, Li S, Han Y, Shen S, Feng Z, Hao P, Li Z, Lin L. Lyu P, et al. Bioorg Chem. 2023 Apr;133:106435. doi: 10.1016/j.bioorg.2023.106435. Epub 2023 Feb 18. Bioorg Chem. 2023. PMID: 36841049
Herein, we synthesized an affinity-based probe of myricanol (pMY) with a photo-affinity cross-linker to initiate a bioconjugation reaction, which was applied for target identification in live C2C12 myotubes. ...
Herein, we synthesized an affinity-based probe of myricanol (pMY) with a photo-affinity cross-linker to initiate a bioconjugation rea …
Identification of Filamin A Mechanobinding Partner III: SAV1 Specifically Interacts with Filamin A Mechanosensitive Domain 21.
Zhang H, Mao Z, Yang Z, Nakamura F. Zhang H, et al. Biochemistry. 2023 Mar 21;62(6):1197-1208. doi: 10.1021/acs.biochem.2c00665. Epub 2023 Mar 1. Biochemistry. 2023. PMID: 36857526
Using the FLNA mechanosensing domains as an affinity ligand followed by stable isotope labeling by amino acids in cell culture (SILAC)-based proteomics, we recently identified smoothelin and fimbacin as FLNA mechanobinding proteins. ...
Using the FLNA mechanosensing domains as an affinity ligand followed by stable isotope labeling by amino acids in cell culture (SILAC)-ba
933 results