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Page 1
Protein precipitation using ammonium sulfate.
Wingfield P. Wingfield P. Curr Protoc Protein Sci. 2001 May;Appendix 3:Appendix 3F. doi: 10.1002/0471140864.psa03fs13. Curr Protoc Protein Sci. 2001. PMID: 18429073 Free PMC article.
The basic theory of protein precipitation by addition of ammonium sulfate is presented and the most common applications are listed. Numerous tables are provided for calculating the appropriate amount of ammonium sulfate to add to a particular protein s …
The basic theory of protein precipitation by addition of ammonium sulfate is presented and the most common applications are li …
Ammonium Sulfate Fractionation of Antibodies.
Fishman JB, Berg EA. Fishman JB, et al. Cold Spring Harb Protoc. 2018 Jun 1;2018(6). doi: 10.1101/pdb.prot099119. Cold Spring Harb Protoc. 2018. PMID: 29858340
Before the widespread availability and use of Protein A (rabbit) or Protein G (rodent) for the purification of IgG, the use of an ammonium sulfate "cut" was the standard method to isolate IgG and other serum proteins. The addition of ammonium sulfate r …
Before the widespread availability and use of Protein A (rabbit) or Protein G (rodent) for the purification of IgG, the use of an ammoniu …
Ammonium sulfate supplementation enhances erythromycin biosynthesis by augmenting intracellular metabolism and precursor supply in Saccharopolyspora erythraea.
Yuan Y, Xu F, Ke X, Lu J, Huang M, Chu J. Yuan Y, et al. Bioprocess Biosyst Eng. 2023 Sep;46(9):1303-1318. doi: 10.1007/s00449-023-02898-x. Epub 2023 Jul 1. Bioprocess Biosyst Eng. 2023. PMID: 37392219
The results suggested that the addition of ammonium sulfate stimulates erythromycin biosynthesis. Targeted metabolomics analysis uncovered that the addition of ammonium sulfate during the late stage of fermentation resulted in an augmented intracellula …
The results suggested that the addition of ammonium sulfate stimulates erythromycin biosynthesis. Targeted metabolomics analys …
Influence of ammonium nitrate on the crystallisation of ammonium sulfate.
Huang B, Dai M, Wen Z, Li W, Zi G, Luo L, Shi Z, Yang L. Huang B, et al. Environ Technol. 2024 Apr;45(11):2196-2204. doi: 10.1080/09593330.2023.2165972. Epub 2023 Jan 17. Environ Technol. 2024. PMID: 36606665
The findings showed that the addition of ammonium nitrate increased the size and aspect ratio of ammonium sulfate crystals. The addition of ammonium nitrate inhibited the dissolution of ammonium sulfate and widened its metastable zone. .. …
The findings showed that the addition of ammonium nitrate increased the size and aspect ratio of ammonium sulfate cryst …
Ammonium sulfate-based prefractionation improved proteome coverage and detection of carbonylated proteins in Arabidopsis thaliana leaf extract.
Tola AJ, Missihoun TD. Tola AJ, et al. Planta. 2023 Feb 19;257(3):62. doi: 10.1007/s00425-023-04083-6. Planta. 2023. PMID: 36808312
Ammonium sulfate is well known to salt out proteins at high concentrations. The study revealed that it can serve to increase by 60% the total number of identified carbonylated proteins by LC-MS/MS. ...In this study, we investigated the hypothesis that a prefractiona …
Ammonium sulfate is well known to salt out proteins at high concentrations. The study revealed that it can serve to increase b …
Protein Precipitation Using Ammonium Sulfate.
[No authors listed] [No authors listed] Curr Protoc Protein Sci. 2016 Apr 1;84:A.3F.1-A.3F.9. doi: 10.1002/0471140864.psa03fs84. Curr Protoc Protein Sci. 2016. PMID: 27038272
The basic theory of protein precipitation by addition of ammonium sulfate is presented, and the most common applications are listed. Tables are provided for calculating the appropriate amount of ammonium sulfate to add to a particular protein solution. …
The basic theory of protein precipitation by addition of ammonium sulfate is presented, and the most common applications are l …
The ammonium sulfate inhibition of human angiogenin.
Chatzileontiadou DS, Tsirkone VG, Dossi K, Kassouni AG, Liggri PG, Kantsadi AL, Stravodimos GA, Balatsos NA, Skamnaki VT, Leonidas DD. Chatzileontiadou DS, et al. FEBS Lett. 2016 Sep;590(17):3005-18. doi: 10.1002/1873-3468.12335. Epub 2016 Aug 12. FEBS Lett. 2016. PMID: 27483019
In this study, we investigate the inhibition of human angiogenin by ammonium sulfate. The inhibitory potency of ammonium sulfate for human angiogenin (IC50 = 123.5 14.9 mm) is comparable to that previously reported for RNase A (119.0 6.5 mm) and RN …
In this study, we investigate the inhibition of human angiogenin by ammonium sulfate. The inhibitory potency of ammonium …
Luminol-hydrogen peroxide-horseradish peroxidase chemiluminescence intensification by kosmotrope ammonium sulfate.
Karatani H. Karatani H. Anal Sci. 2022 Mar;38(3):613-621. doi: 10.1007/s44211-022-00069-8. Epub 2022 Feb 15. Anal Sci. 2022. PMID: 35286630 Free PMC article.
The kosmotropic effect induced by ammonium sulfate (AS) at concentrations greater than approximately 2.8 M allows the marked intensification of chemiluminescence (CL) arising from a conventional luminol-hydrogen peroxide (H(2)O(2))-horseradish peroxidase (HRP) react …
The kosmotropic effect induced by ammonium sulfate (AS) at concentrations greater than approximately 2.8 M allows the marked i …
Enhanced Phytase Production by Bacillus subtilis subsp. subtilis in Solid State Fermentation and its Utility in Improving Food Nutrition.
Singh B, Kumar G, Kumar V, Singh D. Singh B, et al. Protein Pept Lett. 2021;28(10):1083-1089. doi: 10.2174/0929866528666210720142359. Protein Pept Lett. 2021. PMID: 34303326
Bacillus subtilis subsp. subtilis JJBS250 grew well in various agroresidues in SSF and secreted high enzyme titres using wheat bran at 30 C and pH 5.0 after incubation time of 48 h with substrate to moisture ratio of 1:3. Glucose and ammonium sulphate supplementatio …
Bacillus subtilis subsp. subtilis JJBS250 grew well in various agroresidues in SSF and secreted high enzyme titres using wheat bran at 30 C …
22,227 results
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