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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
2012 1
2013 2
2014 1
2015 2
2016 8
2017 14
2018 34
2019 45
2020 49
2021 31
2022 51
2023 44
2024 26

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287 results

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Page 1
Bacteriophage genome engineering with CRISPR-Cas13a.
Guan J, Oromí-Bosch A, Mendoza SD, Karambelkar S, Berry JD, Bondy-Denomy J. Guan J, et al. Nat Microbiol. 2022 Dec;7(12):1956-1966. doi: 10.1038/s41564-022-01243-4. Epub 2022 Oct 31. Nat Microbiol. 2022. PMID: 36316452 Free PMC article.
To provide reverse-genetics tools for DNA jumbo phages from this family, we combined homologous recombination with an RNA-targeting CRISPR-Cas13a enzyme and used an anti-CRISPR gene (acrVIA1) as a selectable marker. We showed that this process can insert foreign gen …
To provide reverse-genetics tools for DNA jumbo phages from this family, we combined homologous recombination with an RNA-targeting CRISPR-C …
Bacteriophages suppress CRISPR-Cas immunity using RNA-based anti-CRISPRs.
Camara-Wilpert S, Mayo-Muñoz D, Russel J, Fagerlund RD, Madsen JS, Fineran PC, Sørensen SJ, Pinilla-Redondo R. Camara-Wilpert S, et al. Nature. 2023 Nov;623(7987):601-607. doi: 10.1038/s41586-023-06612-5. Epub 2023 Oct 18. Nature. 2023. PMID: 37853129 Free PMC article.
We identified Racr candidates for almost all CRISPR-Cas types encoded by a diverse range of viruses and plasmids, often in the genetic context of other anti-CRISPR genes(5). Functional testing of nine candidates spanning the two CRISPR-Cas classes confirmed their st …
We identified Racr candidates for almost all CRISPR-Cas types encoded by a diverse range of viruses and plasmids, often in the genetic conte …
Inhibitory mechanism of CRISPR-Cas9 by AcrIIC4.
Li X, Liao F, Gao J, Song G, Zhang C, Ji N, Wang X, Wen J, He J, Wei Y, Zhang H, Li Z, Yu G, Yin H. Li X, et al. Nucleic Acids Res. 2023 Sep 22;51(17):9442-9451. doi: 10.1093/nar/gkad669. Nucleic Acids Res. 2023. PMID: 37587688 Free PMC article.
CRISPR-Cas systems act as the adaptive immune systems of bacteria and archaea, targeting and destroying invading foreign mobile genetic elements (MGEs) such as phages. MGEs have also evolved anti-CRISPR (Acr) proteins to inactivate the CRISPR-Cas systems. Recently, …
CRISPR-Cas systems act as the adaptive immune systems of bacteria and archaea, targeting and destroying invading foreign mobile genetic elem …
The Anti-Anti-CRISPR.
Torrance DW. Torrance DW. CRISPR J. 2019 Apr;2:79-80. doi: 10.1089/crispr.2019.29049.dwt. CRISPR J. 2019. PMID: 30998088 No abstract available.
Anti-anti-CRISPR.
Tang L. Tang L. Nat Methods. 2019 Nov;16(11):1080. doi: 10.1038/s41592-019-0646-x. Nat Methods. 2019. PMID: 31673149 No abstract available.
Solution structure and dynamics of anti-CRISPR AcrIIA4, the Cas9 inhibitor.
Kim I, Jeong M, Ka D, Han M, Kim NK, Bae E, Suh JY. Kim I, et al. Sci Rep. 2018 Mar 1;8(1):3883. doi: 10.1038/s41598-018-22177-0. Sci Rep. 2018. PMID: 29497118 Free PMC article.
Cas9, an RNA-guided endonuclease, specifically cleaves target DNA substrates and constitutes a well-established platform for genome editing. Recently, anti-CRISPR (Acr) proteins that inhibit Cas9 have been discovered, promising a useful off-switch for Cas9 to avoid …
Cas9, an RNA-guided endonuclease, specifically cleaves target DNA substrates and constitutes a well-established platform for genome editing. …
Light-Inducible CRISPR Labeling.
Hoffmann MD, Bubeck F, Niopek D. Hoffmann MD, et al. Methods Mol Biol. 2020;2173:137-150. doi: 10.1007/978-1-0716-0755-8_9. Methods Mol Biol. 2020. PMID: 32651915
The ability to switch on and off Cas9 DNA binding in CRISPR labeling experiments would thus be important to enable more precise interrogations of the chromatin spatial organization and dynamics and could further be used to study Cas9 DNA binding kinetics directly in living human …
The ability to switch on and off Cas9 DNA binding in CRISPR labeling experiments would thus be important to enable more precise interrogatio …
CRISPR sabotage.
van der Oost J, Brouns SJ. van der Oost J, et al. Genome Biol. 2015 Nov 9;16:248. doi: 10.1186/s13059-015-0820-0. Genome Biol. 2015. PMID: 26553202 Free PMC article.
The CRISPR-Cas system is an example of a prokaryotic immune system in which such co-evolution occurs, as was recently demonstrated by the characterization of a set of viral anti-CRISPR proteins....
The CRISPR-Cas system is an example of a prokaryotic immune system in which such co-evolution occurs, as was recently demonstrated by the ch …
Structural rearrangements allow nucleic acid discrimination by type I-D Cascade.
Schwartz EA, McBride TM, Bravo JPK, Wrapp D, Fineran PC, Fagerlund RD, Taylor DW. Schwartz EA, et al. Nat Commun. 2022 May 20;13(1):2829. doi: 10.1038/s41467-022-30402-8. Nat Commun. 2022. PMID: 35595728 Free PMC article.
We show that type I-D Cascade is capable of specifically binding ssRNA and reveal how PAM recognition of dsDNA targets initiates long-range structural rearrangements that likely primes Cas10d for Cas3' binding and subsequent non-target strand DNA cleavage. These structures allow …
We show that type I-D Cascade is capable of specifically binding ssRNA and reveal how PAM recognition of dsDNA targets initiates long-range …
Structural and mechanistic insights into the CRISPR inhibition of AcrIF7.
Kim I, Koo J, An SY, Hong S, Ka D, Kim EH, Bae E, Suh JY. Kim I, et al. Nucleic Acids Res. 2020 Sep 25;48(17):9959-9968. doi: 10.1093/nar/gkaa690. Nucleic Acids Res. 2020. PMID: 32810226 Free PMC article.
The CRISPR-Cas system provides adaptive immunity for bacteria and archaea to combat invading phages and plasmids. Phages evolved anti-CRISPR (Acr) proteins to neutralize the host CRISPR-Cas immune system as a counter-defense mechanism. ...
The CRISPR-Cas system provides adaptive immunity for bacteria and archaea to combat invading phages and plasmids. Phages evolved anti
287 results