This cross-sectional population-based study in the city of São Paulo, Brazil, aimed to assess prevalence of use of dietary supplements according to life stage and socio-demographic and behavioral characteristics. Prevalence of supplement use was low (6.35%) among the 865 individuals interviewed, with a higher prevalence among women (PR = 1.88; 95%CI: 1.08-1.25). However, no statistically significant difference was found for the other variables. Combined vitamin and mineral supplements headed the list. Intake of supplements is still low in this population. However, the current weak evidence of benefits from regular use and the risks of excessive consumption justify monitoring the use of supplements.