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1,324 results

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Page 1
Bacteriophage genome engineering with CRISPR-Cas13a.
Guan J, Oromí-Bosch A, Mendoza SD, Karambelkar S, Berry JD, Bondy-Denomy J. Guan J, et al. Nat Microbiol. 2022 Dec;7(12):1956-1966. doi: 10.1038/s41564-022-01243-4. Epub 2022 Oct 31. Nat Microbiol. 2022. PMID: 36316452 Free PMC article.
Jumbo phages such as Pseudomonas aeruginosa KZ have potential as antimicrobials and as a model for uncovering basic phage biology. ...Fluorescent tagging of endogenous gp93 revealed that it is ejected with the phage DNA while deletion of the tubulin-like prot …
Jumbo phages such as Pseudomonas aeruginosa KZ have potential as antimicrobials and as a model for uncovering basic phage biol …
Phage-Antibiotic Synergy Is Driven by a Unique Combination of Antibacterial Mechanism of Action and Stoichiometry.
Gu Liu C, Green SI, Min L, Clark JR, Salazar KC, Terwilliger AL, Kaplan HB, Trautner BW, Ramig RF, Maresso AW. Gu Liu C, et al. mBio. 2020 Aug 4;11(4):e01462-20. doi: 10.1128/mBio.01462-20. mBio. 2020. PMID: 32753497 Free PMC article.
The continued rise in antibiotic resistance is precipitating a medical crisis. Bacteriophage (phage) has been hailed as one possible therapeutic option to augment the efficacy of antibiotics. ...Host conditions that simulate the infection environment, including seru …
The continued rise in antibiotic resistance is precipitating a medical crisis. Bacteriophage (phage) has been hailed as one po …
Practical Advice on the One-Step Growth Curve.
Kropinski AM. Kropinski AM. Methods Mol Biol. 2018;1681:41-47. doi: 10.1007/978-1-4939-7343-9_3. Methods Mol Biol. 2018. PMID: 29134585
The one-step growth experiment is fundamental to the description of a new bacteriophage. The following protocol is optimized for those working with rapidly growing bacterial cultures....
The one-step growth experiment is fundamental to the description of a new bacteriophage. The following protocol is optimized for thos …
Phage therapy for treatment of virulent Klebsiella pneumoniae infection in a mouse model.
Anand T, Virmani N, Kumar S, Mohanty AK, Pavulraj S, Bera BC, Vaid RK, Ahlawat U, Tripathi BN. Anand T, et al. J Glob Antimicrob Resist. 2020 Jun;21:34-41. doi: 10.1016/j.jgar.2019.09.018. Epub 2019 Oct 8. J Glob Antimicrob Resist. 2020. PMID: 31604128 Free article.
This study was performed to assess the therapeutic effects of a novel lytic phage (VTCCBPA43) in a pneumonic mouse model in order to explore the efficacy of phage therapy against virulent K. pneumoniae infection. ...Therapeutic efficacy was observed 2 h post-challen …
This study was performed to assess the therapeutic effects of a novel lytic phage (VTCCBPA43) in a pneumonic mouse model in order to …
A Novel Alteromonas Phage Lineage with a Broad Host Range and Small Burst Size.
Yang Y, Ma R, Yu C, Ye J, Chen X, Wang L, Jiao N, Zhang R. Yang Y, et al. Microbiol Spectr. 2022 Aug 31;10(4):e0149922. doi: 10.1128/spectrum.01499-22. Epub 2022 Jul 11. Microbiol Spectr. 2022. PMID: 35862972 Free PMC article.
Our isolation and characterization of these novel phages expand the current understanding of alterophage diversity, evolution, and phage-host interactions. IMPORTANCE The marine bacterium Alteromonas is prevalent in the global ocean with crucial ecological significa …
Our isolation and characterization of these novel phages expand the current understanding of alterophage diversity, evolution, and …
Comprehensive retrospect and future perspective on bacteriophage and cancer.
Liping Z, Sheng Y, Yinhang W, Yifei S, Jiaqun H, Xiaojian Y, Shuwen H, Jing Z. Liping Z, et al. Virol J. 2024 Nov 6;21(1):278. doi: 10.1186/s12985-024-02553-1. Virol J. 2024. PMID: 39501333 Free PMC article.
BACKGROUND: Researchers gradually focus on the relationship between phage and cancer. OBJECTIVE: To summarize the research hotspots and trends in the field of bacteriophage and cancer. ...Future research hotspots should mainly concentrate on the further exploration …
BACKGROUND: Researchers gradually focus on the relationship between phage and cancer. OBJECTIVE: To summarize the research hotspots a …
Characterization of Bacteriophage cd2, a Siphophage Infecting Carnobacterium divergens and a Representative Species of a New Genus of Phage.
Britton AP, Visser KA, Ongenae VMA, Zhang P, Wassink H, Doerksen TA, Welke CA, Lynch KH, van Belkum MJ, Dennis JJ, Yang X, Claessen D, Briegel A, Martin-Visscher LA. Britton AP, et al. Microbiol Spectr. 2023 Aug 17;11(4):e0097323. doi: 10.1128/spectrum.00973-23. Epub 2023 Jul 17. Microbiol Spectr. 2023. PMID: 37458599 Free PMC article.
This phage has a latent period of 60 min and a burst size of ~28 PFU/infected cell. The phage was found to be acid and heat sensitive, with a complete loss of phage activity when stored at pH 2 or heated to 60C. Electron microscopy shows that phage …
This phage has a latent period of 60 min and a burst size of ~28 PFU/infected cell. The phage was found to be acid and …
Isolation, characterization, and genome analysis of a broad host range Salmonella phage vB_SenS_TUMS_E4: a candidate bacteriophage for biocontrol.
Torkashvand N, Kamyab H, Shahverdi AR, Khoshayand MR, Sepehrizadeh Z. Torkashvand N, et al. Vet Res Commun. 2023 Sep;47(3):1493-1503. doi: 10.1007/s11259-023-10105-1. Epub 2023 Apr 25. Vet Res Commun. 2023. PMID: 37097546
Some strains of Salmonella have become progressively resistant to antibiotics, so they could represent a critical threat to public health and have led to the use of alternative therapeutic approaches like phage therapy. In this study, a lytic phage, vB_SenS_TUMS_E4 …
Some strains of Salmonella have become progressively resistant to antibiotics, so they could represent a critical threat to public health an …
Isolation and molecular characterisation of Achromobacter phage phiAxp-3, an N4-like bacteriophage.
Ma Y, Li E, Qi Z, Li H, Wei X, Lin W, Zhao R, Jiang A, Yang H, Yin Z, Yuan J, Zhao X. Ma Y, et al. Sci Rep. 2016 Apr 20;6:24776. doi: 10.1038/srep24776. Sci Rep. 2016. PMID: 27094846 Free PMC article.
Transmission electron microscopy revealed that, with a 67-nm diameter icosahedral head and a 20-nm non-contractile tail, phiAxp-3 has features characteristic of Podoviridae bacteriophages (order Caudovirales). With a burst size of 9000 plaque-forming units and a lat …
Transmission electron microscopy revealed that, with a 67-nm diameter icosahedral head and a 20-nm non-contractile tail, phiAxp-3 has featur …
Phage fibers and spikes: a nanoscale Swiss army knife for host infection.
Ouyang R, Ongenae V, Muok A, Claessen D, Briegel A. Ouyang R, et al. Curr Opin Microbiol. 2024 Feb;77:102429. doi: 10.1016/j.mib.2024.102429. Epub 2024 Jan 26. Curr Opin Microbiol. 2024. PMID: 38277900 Free article. Review.
However, finding a suitable phage relies on numerous factors, including host specificity, burst size, and infection cycle. The host range of a phage is, besides phage defense systems, initially determined by the recognition and attachment of receptor-b …
However, finding a suitable phage relies on numerous factors, including host specificity, burst size, and infection cycle. The …
1,324 results