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Page 1
A novel Borrelia-specific real-time PCR assay is not suitable for diagnosing Lyme neuroborreliosis.
Pedersen RR, Kragh KN, Fritz BG, Ørbæk M, Østrup Jensen P, Lebech AM, Bjarnsholt T. Pedersen RR, et al. Ticks Tick Borne Dis. 2022 Sep;13(5):101971. doi: 10.1016/j.ttbdis.2022.101971. Epub 2022 May 25. Ticks Tick Borne Dis. 2022. PMID: 35649311 Free article.
BACKGROUND: Diagnosing Lyme neuroborreliosis (LNB) is complicated by a lack of adequate test systems and by the complex culturing conditions required to grow the causative pathogens in the Borrelia sensu lato complex. Improved testing methods are urgently needed. He …
BACKGROUND: Diagnosing Lyme neuroborreliosis (LNB) is complicated by a lack of adequate test systems and by the complex culturing conditions …
Borrelia spirochetes in European exotic farm animals.
Hrnková J, Golovchenko M, Musa AS, Needham T, Italiya J, Ceacero F, Kotrba R, Grubhoffer L, Rudenko N, Cerný J. Hrnková J, et al. Front Vet Sci. 2022 Sep 28;9:996015. doi: 10.3389/fvets.2022.996015. eCollection 2022. Front Vet Sci. 2022. PMID: 36246336 Free PMC article.
This study focused on the detection of Borrelia spirochetes (including Borrelia miyamotoi) in exotic farm animals. Using nested-PCR with Borrelia-specific primers, 121 serum samples from 54 exotic farm animals of several species bred in four different …
This study focused on the detection of Borrelia spirochetes (including Borrelia miyamotoi) in exotic farm animals. Using neste …
Comparison of methods for economic and efficient tick and Borrelia DNA purification.
Okeyo M, Hartberger C, Margos G, Straubinger RK, Sing A, Fingerle V. Okeyo M, et al. Ticks Tick Borne Dis. 2019 Aug;10(5):1041-1045. doi: 10.1016/j.ttbdis.2019.05.002. Epub 2019 May 8. Ticks Tick Borne Dis. 2019. PMID: 31171466
DNA yield was estimated in field-collected ticks using conventional PCR targeting the Ixodes Cytochrome C oxidase (coi) gene and in cultured Borrelia isolates using quantitative real-time PCR (qPCR) targeting the FlaB encoding gene of Borrelia. …
DNA yield was estimated in field-collected ticks using conventional PCR targeting the Ixodes Cytochrome C oxidase (coi) gene a …
The real-time PCR targeting the phage terminase (terL) is not suitable for diagnostics of human Borrelia infections in Europe.
Zimmermann M, Margos G, Hartberger C, Lienhard R, Henningsson AJ, Lager M, Markowicz M, Schötta AM, Sing A, Jaulhac B, Lindgren PE, van Dam AP, Hovius JWR, Fingerle V. Zimmermann M, et al. Ticks Tick Borne Dis. 2025 Jul;16(4):102488. doi: 10.1016/j.ttbdis.2025.102488. Epub 2025 Jun 13. Ticks Tick Borne Dis. 2025. PMID: 40516432 Free article.
Here, we re-analysed a previously published real-time PCR targeting the multicopy gene of the large subunit of phage terminase (terL) in Borrelia. We analysed cultured material of Borrelia burgdorferi sl species, serum and clinical tissue samples of LB patien …
Here, we re-analysed a previously published real-time PCR targeting the multicopy gene of the large subunit of phage terminase (terL) …
Using Digital PCR to Unravel the Occurrence of Piroplasmids, Bartonella spp., and Borrelia spp. in Wild Animals from Brazil.
Calchi AC, Mongruel ACB, Cavalcanti FBP, Bartone L, Duarte JMB, Medici EP, Kluyber D, Caiaffa MG, Alves MH, Desbiez ALJ, Coelho TFSB, Machado RZ, Breitschwerdt EB, Maggi RG, André MR. Calchi AC, et al. Pathogens. 2025 Jun 6;14(6):567. doi: 10.3390/pathogens14060567. Pathogens. 2025. PMID: 40559575 Free PMC article.
The genera Bartonella and Borrelia include vector-borne bacteria that can infect and cause disease in both animals and humans. ...The proportions of positive results obtained using dPCR were 85.5% for piroplasmids, 33.6% for Bartonella spp., and 16.7% for Borrelia s …
The genera Bartonella and Borrelia include vector-borne bacteria that can infect and cause disease in both animals and humans. ...The …
Combining citizen science and molecular diagnostic methods to investigate the prevalence of Borrelia burgdorferi s.l. and Borrelia miyamotoi in tick pools across Great Britain.
Shan J, Jia Y, Hickenbotham P, Teulières L, Clokie MRJ. Shan J, et al. Front Microbiol. 2023 Apr 26;14:1126498. doi: 10.3389/fmicb.2023.1126498. eCollection 2023. Front Microbiol. 2023. PMID: 37180256 Free PMC article.
Lyme disease is the most common tick-borne disease and is caused by a group of bacteria known as Borrelia burgdorferi sensu lato (s.l.) complex. ...To investigate the prevalence of B. burgdorferi s.l. and B. miyamotoi in ticks first, we developed a PCR …
Lyme disease is the most common tick-borne disease and is caused by a group of bacteria known as Borrelia burgdorferi sensu la …
Reptile-associated Borrelia species in the goanna tick (Bothriocroton undatum) from Sydney, Australia.
Panetta JL, Šíma R, Calvani NED, Hajdušek O, Chandra S, Panuccio J, Šlapeta J. Panetta JL, et al. Parasit Vectors. 2017 Dec 20;10(1):616. doi: 10.1186/s13071-017-2579-5. Parasit Vectors. 2017. PMID: 29262840 Free PMC article.
BACKGROUND: Knowledge on the capacity of Australian ticks to carry Borrelia species is currently limited or missing. To evaluate the potential of ticks to carry bacterial pathogens and their DNA, it is imperative to have a robust workflow that maximises recov …
BACKGROUND: Knowledge on the capacity of Australian ticks to carry Borrelia species is currently limited or missing. To evalua …
Modification of isothermal test for detecting DNA of pathogens of tick-borne borreliosis.
Olkhovskiy IA, Gorbenko AS, Komarovskiy YY, Stolyar MA, Vasil'eva DI, Epifanova OS, Khodov DA, Rudakova SA, Rudakov NV, Oskorbin IP, Filipenko ML. Olkhovskiy IA, et al. Klin Lab Diagn. 2022 Jan 21;67(1):59-64. doi: 10.51620/0869-2084-2022-67-1-59-64. Klin Lab Diagn. 2022. PMID: 35077072 English.
The copy number of the positive DNA sample of the borrelia plasmid was estimated using digital PCR. The results of the LAMP reaction were compared with those of the commercial PRC-RT test. ...In comparative testing with RT-PCR, the sensitivity o …
The copy number of the positive DNA sample of the borrelia plasmid was estimated using digital PCR. The results of the LAMP re …
The history of Lyme disease in Italy and its spread in the Italian territory.
Trevisan G, Ruscio M, Cinco M, Nan K, Forgione P, Di Meo N, Tranchini P, Nacca M, Trincone S, Rimoldi SG, Giacomet V, Ricci M, Melandri D, Artioli S, Monteforte P, Stinco G, Bonin S. Trevisan G, et al. Front Pharmacol. 2023 Jun 16;14:1128142. doi: 10.3389/fphar.2023.1128142. eCollection 2023. Front Pharmacol. 2023. PMID: 37397497 Free PMC article.
Both diagnoses were confirmed by serological assessment by an indirect immunofluorescence (IFI) technique. Borrelia cultivation from both Ixodes ricinus ticks and human lesions in Trieste (Friuli-Venezia Giulia) identified Borrelia afzelii as the prevalent ge …
Both diagnoses were confirmed by serological assessment by an indirect immunofluorescence (IFI) technique. Borrelia cultivation from …
Evidence of tick-borne organisms in mule deer (Odocoileus hemionus) from the western United States.
Yabsley MJ, Davidson WR, Stallknecht DE, Varela AS, Swift PK, Devos JC Jr, Dubay SA. Yabsley MJ, et al. Vector Borne Zoonotic Dis. 2005 Winter;5(4):351-62. doi: 10.1089/vbz.2005.5.351. Vector Borne Zoonotic Dis. 2005. PMID: 16417431
Free-ranging mule deer (MD; Odocoileus hemionus) from Arizona and California were tested for evidence of infection with several tick-borne pathogens, including species of Ehrlichia, Anaplasma, Babesia, and Borrelia. ...Polymerase chain reaction (PCR) …
Free-ranging mule deer (MD; Odocoileus hemionus) from Arizona and California were tested for evidence of infection with several ti …
35 results