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2020 15
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114 results

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Colorimetric Hybridization Sensor for DNA Mimic of a SARS-CoV-2 RNA Marker: Direct and Inverse Bioanalysis.
Syed ZUQ, Samaraweera S, Wang Z, Schrader JK, Scott C, Schut J, Smith DJ, Ramsey JD, Krishnan S. Syed ZUQ, et al. ACS Meas Sci Au. 2024 Sep 9;4(6):689-694. doi: 10.1021/acsmeasuresciau.4c00043. eCollection 2024 Dec 18. ACS Meas Sci Au. 2024. PMID: 39713035 Free PMC article.
This probe selectively captures a DNA mimic of the SARS-CoV-2 RNA sequence via a base-pair hybridization. The DNA mimic oligomer sequence was tested in a buffer solution, undiluted serum, and undiluted salivary biofluids. A second complementary hybridization …
This probe selectively captures a DNA mimic of the SARS-CoV-2 RNA sequence via a base-pair hybridization. The DNA mimic …
Rapid detection of SARS-CoV-2 RNA using a one-step fast multiplex RT-PCR coupled to lateral flow immunoassay.
Bel Hadj Ali I, Souguir H, Melliti M, Mohamed MVT, Ardhaoui M, Ayouni K, Haddad-Boubaker S, Saadi Ben Aoun Y, Triki H, Guizani I. Bel Hadj Ali I, et al. BMC Infect Dis. 2024 Dec 18;24(1):1417. doi: 10.1186/s12879-024-10296-1. BMC Infect Dis. 2024. PMID: 39695471 Free PMC article.
BACKGROUND: The COVID-19 has put emphasis on pivotal needs for diagnosis and surveillance worldwide, with the subsequent shortage of diagnostic reagents and kits. ...This assay offers a viable alternative for COVID-19 diagnosis or screening at points o …
BACKGROUND: The COVID-19 has put emphasis on pivotal needs for diagnosis and surveillance worldwide, with the subsequent short …
Spatiotemporal protein interactome profiling through condensation-enhanced photocrosslinking.
Li K, Xie X, Gao R, Chen Z, Yang M, Wen Z, Weng Y, Fan X, Zhang G, Liu L, Zeng X, Han Y, Cao M, Wang X, Li J, Yang Z, Li T, Chen PR. Li K, et al. Nat Chem. 2025 Jan;17(1):111-123. doi: 10.1038/s41557-024-01663-1. Epub 2024 Nov 5. Nat Chem. 2025. PMID: 39501047
By leveraging our condensation-enhanced photocrosslinker and the spatially directed biotin tagging, DenseMAP enabled stress-granule-specific interactome mapping of the N(6)-methyladenosine readers YTHDF1 and YTHDF2, and uncovered the functional role of phosphorylation on t …
By leveraging our condensation-enhanced photocrosslinker and the spatially directed biotin tagging, DenseMAP enabled stress-granule-s …
A rapid point-of-care population-scale dipstick assay to identify and differentiate SARS-CoV-2 variants in COVID-19-positive patients.
Paul D, Verma J, Kumar S, Talukdar D, Jana P, Narendrakumar L, Kumar R, Tanwar S, Gosain M, Porey Karmakar S, Pareek M, Mani S, Chaudhuri S, Kshetrapal P, Wadhwa N, Bhatnagar S, Garg PK, Das B. Paul D, et al. Front Microbiol. 2024 Oct 21;15:1459644. doi: 10.3389/fmicb.2024.1459644. eCollection 2024. Front Microbiol. 2024. PMID: 39498137 Free PMC article.
Delta and Omicron variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) are remarkably contagious, and have been recognized as variants of concern (VOC). ...In this study, we analyzed thousands of genome sequences from 30 distinct SARS …
Delta and Omicron variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) are remarkably contagious, a …
Nanoplasmonic microarray-based solid-phase amplification for highly sensitive and multiplexed molecular diagnostics: application for detecting SARS-CoV-2.
Lee JY, Jang H, Kim S, Kang T, Park SG, Lee MY. Lee JY, et al. Mikrochim Acta. 2024 Oct 29;191(11):715. doi: 10.1007/s00604-024-06723-4. Mikrochim Acta. 2024. PMID: 39472332 Free PMC article.
The specificity and sensitivity of solid-phase RPA on nanoplasmonic microarrays was evaluated in detecting E, N, and RdRP genes of SARS-CoV-2. High specificity was achieved by minimizing primer-dimer formation and employing a stringent washing process and hig …
The specificity and sensitivity of solid-phase RPA on nanoplasmonic microarrays was evaluated in detecting E, N, and RdRP genes of SARS …
Profiling of coronaviral Mpro and enteroviral 3Cpro specificity provides a framework for the development of broad-spectrum antiviral compounds.
Rut W, Groborz K, Sun X, Hilgenfeld R, Drag M. Rut W, et al. Protein Sci. 2024 Sep;33(9):e5139. doi: 10.1002/pro.5139. Protein Sci. 2024. PMID: 39150063
However, chemical tools such as substrates, inhibitors, and activity-based probes developed for SARS-CoV-2 M(pro) can be successfully applied to investigate the activity of the M(pro) from other coronaviruses as well as the 3C(pro) from enteroviruses. ...
However, chemical tools such as substrates, inhibitors, and activity-based probes developed for SARS-CoV-2 M(pro) can b …
Development and characterization of a multimeric recombinant protein using the spike protein receptor binding domain as an antigen to induce SARS-CoV-2 neutralization.
de Lima VA, Nunes JPS, Rosa DS, Ferreira R, Oliva MLV, Andreata-Santos R, Duarte-Barbosa M, Janini LMR, Maricato JT, Akamatsu MA, Ho PL, Schenkman S. de Lima VA, et al. Immun Inflamm Dis. 2024 Jul;12(7):e1353. doi: 10.1002/iid3.1353. Immun Inflamm Dis. 2024. PMID: 39056544 Free PMC article.
BACKGROUND: SARS-CoV2 virus, responsible for the COVID-19 pandemic, has four structural proteins and 16 nonstructural proteins. S-protein is one of the structural proteins exposed on the virus surface and is the main target for producing neutralizing antibodies and …
BACKGROUND: SARS-CoV2 virus, responsible for the COVID-19 pandemic, has four structural proteins and 16 nonstructural proteins …
Analytical and Clinical Validation of an Automated Microfluidic Interleukin-6 Immunoassay.
Yang J, Barbhuiya MA, Hamilton C, Robyak K, Zhu Y. Yang J, et al. Ann Clin Lab Sci. 2024 May;54(3):388-393. Ann Clin Lab Sci. 2024. PMID: 39048159
The clinical performance was evaluated in 96 COVID-19 patients. RESULTS: The within-run and between-run coefficient of variations (CV) ranged 1.8%-4.8%. ...Measurement of IL-6 in plasma samples from 45 healthy adults showed the upper limit of reference of 5.0 pg/mL. …
The clinical performance was evaluated in 96 COVID-19 patients. RESULTS: The within-run and between-run coefficient of variati …
TurboID-mediated proximity labeling technologies to identify virus co-receptors.
Wang B, Yang F, Wang W, Zhao F, Sun X. Wang B, et al. Front Cell Infect Microbiol. 2024 Jun 27;14:1371837. doi: 10.3389/fcimb.2024.1371837. eCollection 2024. Front Cell Infect Microbiol. 2024. PMID: 38994005 Free PMC article.
SARS-CoV-2 pseudovirus were incubated with ACE2-TurboID stably expressed cell lines in the presence of biotin and ATP, which could initiate the catalytic activity of TurboID and tag adjacent endogenous proteins with biotin. ...We identified a me …
SARS-CoV-2 pseudovirus were incubated with ACE2-TurboID stably expressed cell lines in the presence of biotin an …
Contributing to the management of viral infections through simple immunosensing of the arachidonic acid serum level.
Torrente-Rodríguez RM, Ruiz-Valdepeñas Montiel V, Iftimie S, Montero-Calle A, Pingarrón JM, Castro A, Camps J, Barderas R, Campuzano S, Joven J. Torrente-Rodríguez RM, et al. Mikrochim Acta. 2024 Jun 4;191(7):369. doi: 10.1007/s00604-024-06440-y. Mikrochim Acta. 2024. PMID: 38834823 Free PMC article.
The designed methodology involves a competitive reaction between the target endogenous ARA and a biotin-ARA competitor for the recognition sites of anti-ARA antibodies covalently attached to the surface of carboxylic acid-coated magnetic microbeads (HOOC-MBs), followed by …
The designed methodology involves a competitive reaction between the target endogenous ARA and a biotin-ARA competitor for the recogn …
114 results