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2020 537
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2025 22

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3,706 results

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An integrated methodological framework for the validation and verification of clinical testing by qRT-PCR.
Cardona-Ramírez C, Enríquez-Valencia CE, Méndez-Callejas G, Barreto GM, Tafur-Gómez GA, Sanjuanelo-Corredor DW. Cardona-Ramírez C, et al. Heliyon. 2024 Dec 15;11(1):e41088. doi: 10.1016/j.heliyon.2024.e41088. eCollection 2025 Jan 15. Heliyon. 2024. PMID: 39801958 Free PMC article.
This paper outlines a practical method for validating quantitative-qualitative techniques used to detect genetic material through qRT-PCR, specifically focusing on SARS-CoV-2 testing and adhering to ISO/IEC 17025:2018 accreditation standards. Despite the prev …
This paper outlines a practical method for validating quantitative-qualitative techniques used to detect genetic material through qRT-PCR, s …
Enhancing chemical signal transformation in lateral flow assays using aptamer-architectured plasmonic nanozymes and para-phenylenediamine.
Sarathkumar E, Jibin K, Sivaselvam S, Sharma AS, Alexandar V, Resmi AN, Velswamy P, Jayasree RS. Sarathkumar E, et al. Nanoscale. 2025 Jan 13. doi: 10.1039/d4nr04130e. Online ahead of print. Nanoscale. 2025. PMID: 39801434
To address these challenges, we developed aptamer-architectured gold nanoparticles as nanozymes that catalytically convert para-phenylenediamine (PPD) into Bandrowski's base (BB), thereby amplifying signal strength and sensitivity. ...The nanozymes and PPD-based LFA test s …
To address these challenges, we developed aptamer-architectured gold nanoparticles as nanozymes that catalytically convert para-pheny …
Neurological post-COVID syndrome is associated with substantial impairment of verbal short-term and working memory.
Charles James J, Schulze H, Siems N, Prehn C, Quast DR, Trampe N, Gold R, Faissner S. Charles James J, et al. Sci Rep. 2025 Jan 11;15(1):1695. doi: 10.1038/s41598-025-85919-x. Sci Rep. 2025. PMID: 39799217 Free PMC article.
A substantial proportion of patients suffer from Post-COVID Syndrome (PCS) with fatigue and impairment of memory and concentration being the most important symptoms. We here set out to perform in-depth neuropsychological assessment of PCS patients referred to the Neurologi …
A substantial proportion of patients suffer from Post-COVID Syndrome (PCS) with fatigue and impairment of memory and concentration be …
Unexpected Discrepancies in the Histopathological and Microbiological Diagnoses of Suspected Mucormycosis Cases at a South Indian Tertiary Care Center During the COVID-19 Pandemic.
Rajyalakshmi R, Athaluri SA, Arava DR, Pyla KR, Manthena SV. Rajyalakshmi R, et al. Cureus. 2024 Dec 10;16(12):e75478. doi: 10.7759/cureus.75478. eCollection 2024 Dec. Cureus. 2024. PMID: 39791083 Free PMC article.
The incidence and prevalence of mucormycosis reached an all-time high during the COVID-19 pandemic due to excessive steroid use and other factors, leading to the coining of the term CAM (COVID Associated Mucormycosis). ...The aim of our research is to study t …
The incidence and prevalence of mucormycosis reached an all-time high during the COVID-19 pandemic due to excessive steroid us …
Detection of the SARS-CoV-2 nucleoprotein by electrochemical biosensor based on molecularly imprinted polypyrrole formed on self-assembled monolayer.
Liustrovaite V, Ratautaite V, Ramanaviciene A, Ramanavicius A. Liustrovaite V, et al. Biosens Bioelectron. 2024 Dec 29;272:117092. doi: 10.1016/j.bios.2024.117092. Online ahead of print. Biosens Bioelectron. 2024. PMID: 39787822
Herein, we report the development and characterisation of an electrochemical biosensor with a polypyrrole (Ppy)-based molecularly imprinted polymer (MIP) for the serological detection of the recombinant nucleocapsid protein of SARS-CoV-2 (rN). The electrochem …
Herein, we report the development and characterisation of an electrochemical biosensor with a polypyrrole (Ppy)-based molecularly imprinted …
Nanobody-Based Lateral Flow Immunoassay for Rapid Antigen Detection of SARS-CoV-2 and MERS-CoV Proteins.
Peng Y, Huang Y, Kiessling F, Renn D, Rueping M. Peng Y, et al. ACS Synth Biol. 2025 Jan 9. doi: 10.1021/acssynbio.4c00592. Online ahead of print. ACS Synth Biol. 2025. PMID: 39786915
The COVID-19 pandemic has highlighted the critical need for pathogen detection methods that offer both low detection limits and rapid results. ...Here, we developed nanobody-based lateral flow assays (LFAs) for the rapid detection of SARS-CoV-2 …
The COVID-19 pandemic has highlighted the critical need for pathogen detection methods that offer both low detection limits an …
Chest Pain With Significantly Elevated Troponins: Be Wary of False Positives.
Narayanan TT, Naneishvili T, Moody W, Townend J, Ludman P. Narayanan TT, et al. Cureus. 2025 Jan 6;17(1):e77018. doi: 10.7759/cureus.77018. eCollection 2025 Jan. Cureus. 2025. PMID: 39777375 Free PMC article.
High-sensitivity cardiac troponins are considered a gold standard for diagnosing acute myocardial infarction and myocardial injury. However, the occurrence of false positives needs to be kept in mind. ...
High-sensitivity cardiac troponins are considered a gold standard for diagnosing acute myocardial infarction and myocardial injury. H …
Automated, Quantitative Capillary Western Blots to Analyze Host Cell Proteins in COVID-19 Vaccine Produced in Vero Cell Line.
Gillespie PF, Wang Y, Yin K, Groegler E, Cunningham N, Stiving AQ, Raffaele J, Marusa N, Tubbs CM, Loughney JW, Winters MA, Rustandi RR. Gillespie PF, et al. Vaccines (Basel). 2024 Dec 5;12(12):1373. doi: 10.3390/vaccines12121373. Vaccines (Basel). 2024. PMID: 39772035 Free PMC article.
METHODS: Herein we describe the development of a capillary western blot method to quantify the HCP content in samples generated using a Vero cell line for the production of an investigational live virus vaccine candidate (V590) for Coronavirus Disease-2019 (COVID-19 …
METHODS: Herein we describe the development of a capillary western blot method to quantify the HCP content in samples generated using a Vero …
CRISPR/Cas12a with Universal crRNA for Indiscriminate Virus Detection.
Shang Z, Liu S, Liu D, Pei X, Li S, He Y, Tong Y, Liu G. Shang Z, et al. Molecules. 2024 Dec 23;29(24):6066. doi: 10.3390/molecules29246066. Molecules. 2024. PMID: 39770154 Free PMC article.
The proposed method allows the same crRNA to detect multiple viral nucleic acids with high sensitivity, including but not limited to SARS-CoV-2, human papillomaviruses (HPV), HCOV-NL63, HCOV-HKU1, and miRNA biomarkers. Taking SARS-CoV-2 a …
The proposed method allows the same crRNA to detect multiple viral nucleic acids with high sensitivity, including but not limited to SARS …
From Tradition to Innovation: Diverse Molecular Techniques in the Fight Against Infectious Diseases.
Alsharksi AN, Sirekbasan S, Gürkök-Tan T, Mustapha A. Alsharksi AN, et al. Diagnostics (Basel). 2024 Dec 21;14(24):2876. doi: 10.3390/diagnostics14242876. Diagnostics (Basel). 2024. PMID: 39767237 Free PMC article. Review.
Molecular biology advancements, notably the polymerase chain reaction (PCR), have revolutionized infectious disease diagnostics by allowing rapid and sensitive detection of pathogens' genetic material. PCR has become the gold standard for many infections, particularly high …
Molecular biology advancements, notably the polymerase chain reaction (PCR), have revolutionized infectious disease diagnostics by allowing …
3,706 results