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emClarity: software for high-resolution cryo-electron tomography and subtomogram averaging.
Himes BA, Zhang P. Himes BA, et al. Nat Methods. 2018 Nov;15(11):955-961. doi: 10.1038/s41592-018-0167-z. Epub 2018 Oct 22. Nat Methods. 2018. PMID: 30349041 Free PMC article.
Macromolecular complexes are intrinsically flexible and often challenging to purify for structure determination by single-particle cryo-electron microscopy (cryo-EM). Such complexes can be studied by cryo-electron tomography (cryo-ET) combined with sub …
Macromolecular complexes are intrinsically flexible and often challenging to purify for structure determination by single-particle cryo …
High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity.
Ni T, Frosio T, Mendonça L, Sheng Y, Clare D, Himes BA, Zhang P. Ni T, et al. Nat Protoc. 2022 Feb;17(2):421-444. doi: 10.1038/s41596-021-00648-5. Epub 2022 Jan 12. Nat Protoc. 2022. PMID: 35022621 Free PMC article. Review.
Cryo-electron tomography and subtomogram averaging (STA) has developed rapidly in recent years. It provides structures of macromolecular complexes in situ and in cellular context at or below subnanometer resolution and has led to unprecedented insights into the inne …
Cryo-electron tomography and subtomogram averaging (STA) has developed rapidly in recent years. It provides structures of macr …
Cryo-electron Tomography Remote Data Collection and Subtomogram Averaging.
Sheng Y, Morris K, Radecke J, Zhang P. Sheng Y, et al. J Vis Exp. 2022 Jul 12;(185):10.3791/63923. doi: 10.3791/63923. J Vis Exp. 2022. PMID: 35913165 Free PMC article.
Cryo-electron tomography (cryo-ET) has been gaining momentum in recent years, especially since the introduction of direct electron detectors, improved automated acquisition strategies, preparative techniques that expand the possibilities of what the electron microsc …
Cryo-electron tomography (cryo-ET) has been gaining momentum in recent years, especially since the introduction of direct elec …