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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1963 2
1965 5
1966 30
1967 76
1968 148
1969 163
1970 210
1971 262
1972 345
1973 373
1974 436
1975 381
1976 318
1977 313
1978 215
1979 260
1980 226
1981 235
1982 252
1983 231
1984 289
1985 286
1986 268
1987 316
1988 322
1989 418
1990 470
1991 496
1992 515
1993 515
1994 532
1995 562
1996 574
1997 585
1998 554
1999 517
2000 537
2001 585
2002 608
2003 582
2004 589
2005 536
2006 547
2007 510
2008 499
2009 519
2010 504
2011 463
2012 465
2013 470
2014 474
2015 502
2016 455
2017 471
2018 428
2019 410
2020 439
2021 444
2022 336
2023 303
2024 332
2025 3

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22,785 results

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Page 1
The Mechanism of Nonenzymatic Template Copying with Imidazole-Activated Nucleotides.
Walton T, Zhang W, Li L, Tam CP, Szostak JW. Walton T, et al. Angew Chem Int Ed Engl. 2019 Aug 5;58(32):10812-10819. doi: 10.1002/anie.201902050. Epub 2019 Jul 4. Angew Chem Int Ed Engl. 2019. PMID: 30908802 Review.
An important model for the study of nonenzymatic template copying, which would be a key part of any such pathway, involves the reaction of ribonucleoside-5'-phosphorimidazolides with an RNA primer/template complex. ...Here we review the discovery of this unexpected …
An important model for the study of nonenzymatic template copying, which would be a key part of any such pathway, involves the reacti …
Diaminopurine in Nonenzymatic RNA Template Copying.
Jia X, Fang Z, Kim SC, Ding D, Zhou L, Szostak JW. Jia X, et al. J Am Chem Soc. 2024 Jun 12;146(23):15897-15907. doi: 10.1021/jacs.4c02560. Epub 2024 May 31. J Am Chem Soc. 2024. PMID: 38818863 Free PMC article.
In the RNA World before the emergence of an RNA polymerase, nonenzymatic template copying would have been essential for the transmission of genetic information. ...We therefore asked whether replacing adenine (A) with diaminopurine (D) might lead to more efficient a …
In the RNA World before the emergence of an RNA polymerase, nonenzymatic template copying would have been essential for the transmiss …
Template-Directed Copying of RNA by Non-enzymatic Ligation.
Zhou L, O'Flaherty DK, Szostak JW. Zhou L, et al. Angew Chem Int Ed Engl. 2020 Sep 1;59(36):15682-15687. doi: 10.1002/anie.202004934. Epub 2020 Aug 13. Angew Chem Int Ed Engl. 2020. PMID: 32558121 Free PMC article.
The non-enzymatic replication of the primordial genetic material is thought to have enabled the evolution of early forms of RNA-based life. However, the replication of oligonucleotides long enough to encode catalytic functions is problematic due to the low efficiency of …
The non-enzymatic replication of the primordial genetic material is thought to have enabled the evolution of early forms of RNA-based …
Best Practices for Illumina Library Preparation.
Bronner IF, Quail MA. Bronner IF, et al. Curr Protoc Hum Genet. 2019 Jun;102(1):e86. doi: 10.1002/cphg.86. Curr Protoc Hum Genet. 2019. PMID: 31216112 Review.
In this unit, we describe a set of protocols and recommendations for Illumina library preparation. We review best practices in template quantitation methods; template fragmentation methodologies; solid-phase reverse-immobilization cleanup, including buffer exchange …
In this unit, we describe a set of protocols and recommendations for Illumina library preparation. We review best practices in template …
Template-independent DNA polymerases.
Yue D, Tabor S, Nichols NM. Yue D, et al. Curr Protoc Mol Biol. 2008 Oct;Chapter 3:Unit3.6. doi: 10.1002/0471142727.mb0306s84. Curr Protoc Mol Biol. 2008. PMID: 18972388
Terminal deoxynucleotidyl transferase (TdT), is a template-independent DNA polymerase that catalyzes the incorporation of deoxynucleotides at the 3'-hydroxyl terminus of DNA, accompanied by the release of inorganic phosphate. TdT does not require a template and will …
Terminal deoxynucleotidyl transferase (TdT), is a template-independent DNA polymerase that catalyzes the incorporation of deoxynucleo …
DNA-catalyzed polymerization.
Li X, Zhan ZY, Knipe R, Lynn DG. Li X, et al. J Am Chem Soc. 2002 Feb 6;124(5):746-7. doi: 10.1021/ja017319j. J Am Chem Soc. 2002. PMID: 11817938
The reaction follows step-growth kinetics to read the encoded sequence and chain-length information in the antiparallel direction. Single mismatches in the template are selected against at a level of >100:1. A method is therefore established to translate biopolymer-enco …
The reaction follows step-growth kinetics to read the encoded sequence and chain-length information in the antiparallel direction. Single mi …
Prolinyl Nucleotides Drive Enzyme-Free Genetic Copying of RNA.
Welsch F, Kervio E, Tremmel P, Richert C. Welsch F, et al. Angew Chem Int Ed Engl. 2023 Oct 9;62(41):e202307591. doi: 10.1002/anie.202307591. Epub 2023 Jul 11. Angew Chem Int Ed Engl. 2023. PMID: 37382466
Both dinucleotides and mononucleotides are incorporated at the terminus of RNA primers in aqueous buffer, as instructed by the template sequence, in up to eight consecutive extension steps. Our results show that condensation products of amino acids and ribonucleotides can …
Both dinucleotides and mononucleotides are incorporated at the terminus of RNA primers in aqueous buffer, as instructed by the template …
Quantitating primer-template interactions using deconstructed PCR.
Kahsen J, Sherwani SK, Naqib A, Jeon T, Wu LYA, Green SJ. Kahsen J, et al. PeerJ. 2024 Aug 8;12:e17787. doi: 10.7717/peerj.17787. eCollection 2024. PeerJ. 2024. PMID: 39131619 Free PMC article.
This bias can be influenced by mismatches between primers and templates, the locations of mismatches, and the nucleotide pairing of mismatches. Many studies have examined primer-template interactions through interrogation of the final products of PCR amplification w …
This bias can be influenced by mismatches between primers and templates, the locations of mismatches, and the nucleotide pairing of m …
Template-independent DNA polymerases.
Tabor S. Tabor S. Curr Protoc Mol Biol. 2001 May;Chapter 3:Unit3.6. doi: 10.1002/0471142727.mb0306s00. Curr Protoc Mol Biol. 2001. PMID: 18265232
Amplification by nucleic acid-templated reactions.
Michaelis J, Roloff A, Seitz O. Michaelis J, et al. Org Biomol Chem. 2014 May 14;12(18):2821-33. doi: 10.1039/c4ob00096j. Org Biomol Chem. 2014. PMID: 24671414 Review.
Such reaction systems are currently being explored for applications in DNA/RNA diagnosis, drug screening and as a means to design gene expression specific therapy. However, biological nucleic acid templates usually have low abundance. Therefore, either the targeted nucleic …
Such reaction systems are currently being explored for applications in DNA/RNA diagnosis, drug screening and as a means to design gene expre …
22,785 results
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