Survey of aflatoxins in rice from Iran using immunoaffinity column clean-up and HPLC with fluorescence detection

Food Addit Contam Part B Surveill. 2010;3(4):263-7. doi: 10.1080/19440049.2010.516456.

Abstract

A study was undertaken to determine levels of aflatoxins in rice. A total of 261 rice samples were analyzed by HPLC using a method was based on the extraction of 50 g of finely ground rice plus 5 g NaCl with 200 ml of 80% methanol. After filtration and immunoaffinity clean-up, 20 µl was injected onto the HPLC. HPLC analysis was carried out using a Genesis RP C18 column (250 × 4.6, 4 µm I.D.) and a mobile phase with a linear gradient of water/methanol/acetonitrile (6 : 2 : 2 v/v) over 16 min. Aflatoxins were determined after post-column derivatisation with iodine by fluorescence detection at excitation and emission wavelengths of 365 and 445 nm, respectively. It was found that 68.9% of the rice samples contained aflatoxin B1 at levels greater than 0.2 ng g(-1).

Keywords: aflatoxins; chromatography, HPLC; quality assurance; rice; risk assessment; toxicology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aflatoxin B1 / analysis
  • Aflatoxins / analysis*
  • Aminolevulinic Acid / analogs & derivatives*
  • Aminolevulinic Acid / chemistry
  • Chromatography, Affinity / methods*
  • Chromatography, High Pressure Liquid / methods*
  • Food Analysis / methods*
  • Food Contamination / analysis*
  • Iran
  • Maximum Allowable Concentration
  • Seeds / chemistry
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence

Substances

  • Aflatoxins
  • 2-amino-5-hydroxy-4-oxopentanoic acid
  • Aminolevulinic Acid
  • Aflatoxin B1