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2011 1
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16 results

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Page 1
Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafish.
Shin M, Yin HM, Shih YH, Nozaki T, Portman D, Toles B, Kolb A, Luk K, Isogai S, Ishida K, Hanasaka T, Parsons MJ, Wolfe SA, Burns CE, Burns CG, Lawson ND. Shin M, et al. Dev Cell. 2023 Nov 20;58(22):2614-2626.e7. doi: 10.1016/j.devcel.2023.07.022. Epub 2023 Aug 25. Dev Cell. 2023. PMID: 37633272
The zebrafish is amenable to a variety of genetic approaches. However, lack of conditional deletion alleles limits stage- or cell-specific gene knockout. ...Together, our results provide a framework for routine generation and application of endogenously floxed alleles in …
The zebrafish is amenable to a variety of genetic approaches. However, lack of conditional deletion alleles limits stage- or cell-spe …
Back and forth: History of and new insights on the vertebrate lymphatic valve.
Shin M, Lawson ND. Shin M, et al. Dev Growth Differ. 2021 Dec;63(9):523-535. doi: 10.1111/dgd.12757. Epub 2021 Nov 16. Dev Growth Differ. 2021. PMID: 34716915 Free PMC article. Review.
However, we have recently used a combination of imaging and genetic analysis in the zebrafish to demonstrate that valves are a conserved feature of the teleost lymphatic system. Here, we provide a historical overview of comparative lymphatic valve anatomy together with rec …
However, we have recently used a combination of imaging and genetic analysis in the zebrafish to demonstrate that valves are a conser …
5'-Modifications improve potency and efficacy of DNA donors for precision genome editing.
Ghanta KS, Chen Z, Mir A, Dokshin GA, Krishnamurthy PM, Yoon Y, Gallant J, Xu P, Zhang XO, Ozturk AR, Shin M, Idrizi F, Liu P, Gneid H, Edraki A, Lawson ND, Rivera-Pérez JA, Sontheimer EJ, Watts JK, Mello CC. Ghanta KS, et al. Elife. 2021 Oct 19;10:e72216. doi: 10.7554/eLife.72216. Elife. 2021. PMID: 34665130 Free PMC article.
We describe 5'-terminal modifications, including in its simplest form the incorporation of triethylene glycol (TEG) moieties, that consistently increase the frequency of precision editing in the germlines of three animal models (Caenorhabditis elegans, zebrafish, mice) and …
We describe 5'-terminal modifications, including in its simplest form the incorporation of triethylene glycol (TEG) moieties, that consisten …
VEGFC/FLT4-induced cell-cycle arrest mediates sprouting and differentiation of venous and lymphatic endothelial cells.
Jerafi-Vider A, Bassi I, Moshe N, Tevet Y, Hen G, Splittstoesser D, Shin M, Lawson ND, Yaniv K. Jerafi-Vider A, et al. Cell Rep. 2021 Jun 15;35(11):109255. doi: 10.1016/j.celrep.2021.109255. Cell Rep. 2021. PMID: 34133928 Free PMC article.
Here, we identify cell cycle progression as a regulator of EC sprouting and differentiation. Using transgenic zebrafish illuminating cell cycle stages, we show that venous and lymphatic precursors sprout from the cardinal vein exclusively in G1 and reveal that cell-cycle a …
Here, we identify cell cycle progression as a regulator of EC sprouting and differentiation. Using transgenic zebrafish illuminating …
An improved zebrafish transcriptome annotation for sensitive and comprehensive detection of cell type-specific genes.
Lawson ND, Li R, Shin M, Grosse A, Yukselen O, Stone OA, Kucukural A, Zhu L. Lawson ND, et al. Elife. 2020 Aug 24;9:e55792. doi: 10.7554/eLife.55792. Elife. 2020. PMID: 32831172 Free PMC article.
The zebrafish is ideal for studying embryogenesis and is increasingly applied to model human disease. ...Thus, we demonstrate that our new transcriptome annotation can outperform existing annotations, providing an important resource for zebrafish researchers....
The zebrafish is ideal for studying embryogenesis and is increasingly applied to model human disease. ...Thus, we demonstrate that ou …
Valves Are a Conserved Feature of the Zebrafish Lymphatic System.
Shin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S, Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND. Shin M, et al. Dev Cell. 2019 Nov 4;51(3):374-386.e5. doi: 10.1016/j.devcel.2019.08.019. Epub 2019 Sep 26. Dev Cell. 2019. PMID: 31564611 Free PMC article.
Zebrafish embryos bearing mutations in genes required for mammalian valve morphogenesis show defective lymphatic valve formation and edema. Together, our observations provide a foundation from which to further investigate lymphatic valve formation in zebrafish....
Zebrafish embryos bearing mutations in genes required for mammalian valve morphogenesis show defective lymphatic valve formation and …
Enhanced Cas12a editing in mammalian cells and zebrafish.
Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh S, Morrison K, Frazão JB, Bolukbasi MF, Ponnienselvan K, Luban J, Zhu LJ, Lawson ND, Wolfe SA. Liu P, et al. Nucleic Acids Res. 2019 May 7;47(8):4169-4180. doi: 10.1093/nar/gkz184. Nucleic Acids Res. 2019. PMID: 30892626 Free PMC article.
Here we describe improvements to the Cas12a system that facilitate efficient targeted mutagenesis in mammalian cells and zebrafish embryos. We show that engineered variants of Cas12a with two different nuclear localization sequences (NLS) on the C terminus provide increase …
Here we describe improvements to the Cas12a system that facilitate efficient targeted mutagenesis in mammalian cells and zebrafish em …
Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors.
Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND. Shin M, et al. Development. 2016 Oct 15;143(20):3785-3795. doi: 10.1242/dev.137901. Epub 2016 Sep 12. Development. 2016. PMID: 27621059 Free PMC article.
However, the signals that act downstream of Flt4 in this context in vivo remain unclear. To understand Flt4 signaling better, we generated zebrafish bearing a deletion in the Flt4 cytoplasmic domain that eliminates tyrosines Y1226 and 1227. ...Thus, facial lymphatic vessel …
However, the signals that act downstream of Flt4 in this context in vivo remain unclear. To understand Flt4 signaling better, we generated …
Vegfa signals through ERK to promote angiogenesis, but not artery differentiation.
Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND. Shin M, et al. Development. 2016 Oct 15;143(20):3796-3805. doi: 10.1242/dev.137919. Epub 2016 Aug 30. Development. 2016. PMID: 27578780 Free PMC article.
To address this issue, we have visualized activation of presumptive Vegfa effectors at single-cell resolution in zebrafish blood vessels. From these studies, we find that phosphorylation of the serine/threonine kinase ERK (pERK) preferentially occurs in endothelial cells u …
To address this issue, we have visualized activation of presumptive Vegfa effectors at single-cell resolution in zebrafish blood vess …
Venous-derived angioblasts generate organ-specific vessels during zebrafish embryonic development.
Hen G, Nicenboim J, Mayseless O, Asaf L, Shin M, Busolin G, Hofi R, Almog G, Tiso N, Lawson ND, Yaniv K. Hen G, et al. Development. 2015 Dec 15;142(24):4266-78. doi: 10.1242/dev.129247. Epub 2015 Nov 2. Development. 2015. PMID: 26525671 Free PMC article.
Here, we show that both arterial and venous components of the subintestinal plexus originate from a pool of specialized angioblasts residing in the floor of the posterior cardinal vein (PCV). Using live imaging of zebrafish embryos, in combination with photoconvertable tra …
Here, we show that both arterial and venous components of the subintestinal plexus originate from a pool of specialized angioblasts residing …
16 results