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64 results

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Page 1
Photoactivatable drugs for nicotinic optopharmacology.
Banala S, Arvin MC, Bannon NM, Jin XT, Macklin JJ, Wang Y, Peng C, Zhao G, Marshall JJ, Gee KR, Wokosin DL, Kim VJ, McIntosh JM, Contractor A, Lester HA, Kozorovitskiy Y, Drenan RM, Lavis LD. Banala S, et al. Among authors: lester ha. Nat Methods. 2018 May;15(5):347-350. doi: 10.1038/nmeth.4637. Epub 2018 Mar 26. Nat Methods. 2018. PMID: 29578537 Free PMC article.
Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning.
Unger EK, Keller JP, Altermatt M, Liang R, Matsui A, Dong C, Hon OJ, Yao Z, Sun J, Banala S, Flanigan ME, Jaffe DA, Hartanto S, Carlen J, Mizuno GO, Borden PM, Shivange AV, Cameron LP, Sinning S, Underhill SM, Olson DE, Amara SG, Temple Lang D, Rudnick G, Marvin JS, Lavis LD, Lester HA, Alvarez VA, Fisher AJ, Prescher JA, Kash TL, Yarov-Yarovoy V, Gradinaru V, Looger LL, Tian L. Unger EK, et al. Among authors: lester ha. Cell. 2020 Dec 23;183(7):1986-2002.e26. doi: 10.1016/j.cell.2020.11.040. Epub 2020 Dec 16. Cell. 2020. PMID: 33333022 Free PMC article.
We developed and applied a binding-pocket redesign strategy, guided by machine learning, to create a high-performance, soluble, fluorescent serotonin sensor (iSeroSnFR), enabling optical detection of millisecond-scale serotonin transients. ...
We developed and applied a binding-pocket redesign strategy, guided by machine learning, to create a high-performance, soluble, fluoresce …
Selective Serotonin Reuptake Inhibitors within Cells: Temporal Resolution in Cytoplasm, Endoplasmic Reticulum, and Membrane.
Nichols AL, Blumenfeld Z, Luebbert L, Knox HJ, Muthusamy AK, Marvin JS, Kim CH, Grant SN, Walton DP, Cohen BN, Hammar R, Looger L, Artursson P, Dougherty DA, Lester HA. Nichols AL, et al. Among authors: lester ha. J Neurosci. 2023 Mar 29;43(13):2222-2241. doi: 10.1523/JNEUROSCI.1519-22.2022. Epub 2023 Mar 3. J Neurosci. 2023. PMID: 36868853 Free PMC article.
We studied escitalopram and fluoxetine using new intensity-based, drug-sensing fluorescent reporters targeted to the plasma membrane, cytoplasm, or endoplasmic reticulum (ER) of cultured neurons and mammalian cell lines. ...
We studied escitalopram and fluoxetine using new intensity-based, drug-sensing fluorescent reporters targeted to the plasma membrane, …
Fluorescence activation mechanism and imaging of drug permeation with new sensors for smoking-cessation ligands.
Nichols AL, Blumenfeld Z, Fan C, Luebbert L, Blom AEM, Cohen BN, Marvin JS, Borden PM, Kim CH, Muthusamy AK, Shivange AV, Knox HJ, Campello HR, Wang JH, Dougherty DA, Looger LL, Gallagher T, Rees DC, Lester HA. Nichols AL, et al. Among authors: lester ha. Elife. 2022 Jan 4;11:e74648. doi: 10.7554/eLife.74648. Elife. 2022. PMID: 34982029 Free PMC article.
Here, we developed new intensity-based drug-sensing fluorescent reporters (iDrugSnFRs) for the nicotinic partial agonists dianicline, cytisine, and two cytisine derivatives - 10-fluorocytisine and 9-bromo-10-ethylcytisine. ...At the PM, the speed of solution changes limits …
Here, we developed new intensity-based drug-sensing fluorescent reporters (iDrugSnFRs) for the nicotinic partial agonists dianicline, …
Fluorescence Screens for Identifying Central Nervous System-Acting Drug-Biosensor Pairs for Subcellular and Supracellular Pharmacokinetics.
Beatty ZG, Muthusamy AK, Unger EK, Dougherty DA, Tian L, Looger LL, Shivange AV, Bera K, Lester HA, Nichols AL. Beatty ZG, et al. Among authors: lester ha. Bio Protoc. 2022 Nov 20;12(22):e4551. doi: 10.21769/BioProtoc.4551. eCollection 2022 Nov 20. Bio Protoc. 2022. PMID: 36532688 Free PMC article.
Recent investigations have been enhanced by the use of genetically encoded fluorescent biosensors for drugs of interest at the plasma membrane and in organelles. We describe screening and validation protocols for identifying hit pairs comprising a drug and biosensor, with …
Recent investigations have been enhanced by the use of genetically encoded fluorescent biosensors for drugs of interest at the plasma …
Correction: Fluorescence activation mechanism and imaging of drug permeation with new sensors for smoking-cessation ligands.
Nichols AL, Blumenfeld Z, Fan C, Luebbert L, Blom AEM, Cohen BN, Marvin JS, Borden PM, Kim CH, Muthusamy AK, Shivange AV, Knox HJ, Campello HR, Wang JH, Dougherty DA, Looger LL, Gallagher T, Rees DC, Lester HA. Nichols AL, et al. Among authors: lester ha. Elife. 2022 Dec 15;11:e85479. doi: 10.7554/eLife.85479. Elife. 2022. PMID: 36520038 Free PMC article.
An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy.
Khakh BS, Fisher JA, Nashmi R, Bowser DN, Lester HA. Khakh BS, et al. Among authors: lester ha. J Neurosci. 2005 Jul 20;25(29):6911-20. doi: 10.1523/JNEUROSCI.0561-05.2005. J Neurosci. 2005. PMID: 16033901 Free PMC article.
Here, we show that plasma membrane alpha4beta2 nicotinic and P2X2 channels form a molecular scale partnership and also influence each other when coactivated, resulting in nonadditive cross-inhibitory responses. Total internal reflection fluorescence and fluorescence …
Here, we show that plasma membrane alpha4beta2 nicotinic and P2X2 channels form a molecular scale partnership and also influence each other …
Early fluorescence signals detect transitions at mammalian serotonin transporters.
Li M, Lester HA. Li M, et al. Among authors: lester ha. Biophys J. 2002 Jul;83(1):206-18. doi: 10.1016/S0006-3495(02)75162-X. Biophys J. 2002. PMID: 12080113 Free PMC article.
The fluorescence also increases with membrane depolarization. During voltage-jump experiments, fluorescence relaxations show little inactivation or history dependence. The fluorescence signal has a voltage dependence similar to that of the prepriming step of …
The fluorescence also increases with membrane depolarization. During voltage-jump experiments, fluorescence relaxations show l …
Three Mutations Convert the Selectivity of a Protein Sensor from Nicotinic Agonists to S-Methadone for Use in Cells, Organelles, and Biofluids.
Muthusamy AK, Kim CH, Virgil SC, Knox HJ, Marvin JS, Nichols AL, Cohen BN, Dougherty DA, Looger LL, Lester HA. Muthusamy AK, et al. Among authors: lester ha. J Am Chem Soc. 2022 May 18;144(19):8480-8486. doi: 10.1021/jacs.2c02323. Epub 2022 Apr 21. J Am Chem Soc. 2022. PMID: 35446570 Free PMC article.
We report a reagentless, intensity-based S-methadone fluorescent sensor, iS-methadoneSnFR, consisting of a circularly permuted GFP inserted within the sequence of a mutated bacterial periplasmic binding protein (PBP). ...
We report a reagentless, intensity-based S-methadone fluorescent sensor, iS-methadoneSnFR, consisting of a circularly permuted GFP in …
Biosensors Show the Pharmacokinetics of S-Ketamine in the Endoplasmic Reticulum.
Bera K, Kamajaya A, Shivange AV, Muthusamy AK, Nichols AL, Borden PM, Grant S, Jeon J, Lin E, Bishara I, Chin TM, Cohen BN, Kim CH, Unger EK, Tian L, Marvin JS, Looger LL, Lester HA. Bera K, et al. Among authors: lester ha. Front Cell Neurosci. 2019 Nov 12;13:499. doi: 10.3389/fncel.2019.00499. eCollection 2019. Front Cell Neurosci. 2019. PMID: 31798415 Free PMC article.
The tools are genetically encoded intensity-based S-ketamine-sensing fluorescent reporters, iSKetSnFR1 and iSKetSnFR2. In solution, these biosensors respond to S-ketamine with a sensitivity, S-slope = delta(F/F(0))/(delta[S-ketamine]) of 0.23 and 1.9/muM, respectively. ...
The tools are genetically encoded intensity-based S-ketamine-sensing fluorescent reporters, iSKetSnFR1 and iSKetSnFR2. In solution, t …
64 results