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Exogenous myrosinase from mustard seed increases bioavailability of sulforaphane from a glucoraphanin-rich broccoli seed extract in a randomized clinical study.
Mastaloudis A, Holcomb L, Fahey JW, Sakaguchi CA, Nieman DC, Kay C, O'Donnell R, Pecorelli A, Kinney M, Li Y, Ishaq SL, Valacchi G. Mastaloudis A, et al. Sci Rep. 2026 Feb 15;16(1):9162. doi: 10.1038/s41598-026-39389-4. Sci Rep. 2026. PMID: 41692762 Free PMC article. Clinical Trial.
In a randomized, double-blind, crossover study, sixteen subjects (9 F: 7 M) received a single oral dose of GR in broccoli seed extract with Myr-containing mustard seed powder, or broccoli seed extract alone, both with ascorbic acid. ...To our knowledge …
In a randomized, double-blind, crossover study, sixteen subjects (9 F: 7 M) received a single oral dose of GR in broccoli seed extrac …
Release of allyl isothiocyanate from mustard seed meal powder.
Dai R, Lim LT. Dai R, et al. J Food Sci. 2014 Jan;79(1):E47-53. doi: 10.1111/1750-3841.12322. Epub 2013 Dec 6. J Food Sci. 2014. PMID: 24313968
Allyl isothiocyanate (AITC) is a wide-spectrum antimicrobial compound found in mustard seeds, produced when their tissues are disrupted. The formation of AITC in mustard seed is mediated by the myrosinase enzyme which catalyzes the releas …
Allyl isothiocyanate (AITC) is a wide-spectrum antimicrobial compound found in mustard seeds, produced when their tissu …
Allyl isothiocyanate-rich mustard seed powder inhibits bladder cancer growth and muscle invasion.
Bhattacharya A, Li Y, Wade KL, Paonessa JD, Fahey JW, Zhang Y. Bhattacharya A, et al. Carcinogenesis. 2010 Dec;31(12):2105-10. doi: 10.1093/carcin/bgq202. Epub 2010 Oct 1. Carcinogenesis. 2010. PMID: 20889681 Free PMC article.
The present investigation was designed to test the hypothesis that AITC-containing cruciferous vegetables also inhibit bladder cancer development. We focused on an AITC-rich mustard seed powder (MSP-1). AITC was stably stored as its glucosinolate precursor (sinigrin …
The present investigation was designed to test the hypothesis that AITC-containing cruciferous vegetables also inhibit bladder cancer develo …
Supercritical fluid chromatography of myrosinase reaction products in ground yellow mustard seed oil.
Choubdar N, Li S, Holley RA. Choubdar N, et al. J Food Sci. 2010 May;75(4):C341-5. doi: 10.1111/j.1750-3841.2010.01584.x. J Food Sci. 2010. PMID: 20546392
Compounds generated by enzymatic hydrolysis of glucosinolates present in ground mustard have shown potential antibacterial properties against E. coli O157:H7. In the present study, supercritical fluid chromatography (SFC) was used to investigate degradation products of 4-h …
Compounds generated by enzymatic hydrolysis of glucosinolates present in ground mustard have shown potential antibacterial properties …
Supplementation of the Diet by Exogenous Myrosinase via Mustard Seeds to Increase the Bioavailability of Sulforaphane in Healthy Human Subjects after the Consumption of Cooked Broccoli.
Okunade O, Niranjan K, Ghawi SK, Kuhnle G, Methven L. Okunade O, et al. Mol Nutr Food Res. 2018 Sep;62(18):e1700980. doi: 10.1002/mnfr.201700980. Epub 2018 Jul 26. Mol Nutr Food Res. 2018. PMID: 29806738 Clinical Trial.
SCOPE: Broccoli contains glucosinolate glucoraphanin, which, in the presence of myrosinase, can hydrolyze to isothiocyanate sulforaphane, reported to have anticarcinogenic activity. However, the myrosinase enzyme is denatured on cooking. Addition of an active …
SCOPE: Broccoli contains glucosinolate glucoraphanin, which, in the presence of myrosinase, can hydrolyze to isothiocyanate su …
The effect of processing on the glucosinolate profile in mustard seed.
Cools K, Terry LA. Cools K, et al. Food Chem. 2018 Jun 30;252:343-348. doi: 10.1016/j.foodchem.2018.01.096. Epub 2018 Jan 16. Food Chem. 2018. PMID: 29478552
Brassica juncea mustard seed are used to make mustard paste or condiment. Mustard seed contains glucosinolates which are converted to isothiocyanates following cell disruption by the enzyme, myrosinase. ...Three mustard s …
Brassica juncea mustard seed are used to make mustard paste or condiment. Mustard seed contains glucosino …
Anti-adipogenic Effects of Sulforaphane-rich Ingredient with Broccoli Sprout and Mustard Seed in 3T3-L1 Preadipocytes.
Men X, Han X, Lee SJ, Park KT, Han JK, Choi SI, Lee OH. Men X, et al. Planta Med. 2023 Apr;89(5):526-538. doi: 10.1055/a-1853-7101. Epub 2022 May 16. Planta Med. 2023. PMID: 35577064
Glucoraphanin (GRA) is a precursor of sulforaphane (SFN), which can be synthesized by the enzyme myrosinase. In this study, we developed and validated HPLC analytical methods for the determination of GRA and SFN in mustard seed powder (MSP), broccoli sprout p …
Glucoraphanin (GRA) is a precursor of sulforaphane (SFN), which can be synthesized by the enzyme myrosinase. In this study, we develo …
Inactivation of Thioglucosidase from Sinapis alba (White Mustard) Seed by Metal Salts.
Marcinkowska M, Jeleń HH. Marcinkowska M, et al. Molecules. 2020 Sep 23;25(19):4363. doi: 10.3390/molecules25194363. Molecules. 2020. PMID: 32977439 Free PMC article.
The glucosinolates which are specialized plant metabolites of Brassica vegetables are prone to hydrolysis catalyzed by an endogenous enzyme myrosinase (thioglycoside hydrolase, thioglucosidase) that exists in Brassica plant tissue causing volatile i …
The glucosinolates which are specialized plant metabolites of Brassica vegetables are prone to hydrolysis catalyzed by an endogenous …
Brassica Nigra: Ethopharmacological Review of a Routinely Used Condiment.
Agrawal S, Yallatikar T, Gurjar P. Agrawal S, et al. Curr Drug Discov Technol. 2019;16(1):40-47. doi: 10.2174/1570163815666180308143400. Curr Drug Discov Technol. 2019. PMID: 29521240 Review.
BACKGROUND: Brassica nigra belonging to the family Brassicaceae (syn - black mustard) comprises of dried seeds and is widely cultivated in Mediterranean region and various other countries like India and Europe. ...RESULTS: B. nigra contains glycosinolates which unde …
BACKGROUND: Brassica nigra belonging to the family Brassicaceae (syn - black mustard) comprises of dried seeds and is widely c …
Purification of active myrosinase from plants by aqueous two-phase counter-current chromatography.
Wade KL, Ito Y, Ramarathnam A, Holtzclaw WD, Fahey JW. Wade KL, et al. Phytochem Anal. 2015 Jan-Feb;26(1):47-53. doi: 10.1002/pca.2535. Epub 2014 Aug 7. Phytochem Anal. 2015. PMID: 25130502 Free PMC article.
INTRODUCTION: Myrosinase (thioglucoside glucohydrolase; E.C. 3.2.1.147), is a plant enzyme of increasing interest and importance to the biomedical community. ...Optimum phase retention and separation of myrosinase from other plant proteins afforded a 6 …
INTRODUCTION: Myrosinase (thioglucoside glucohydrolase; E.C. 3.2.1.147), is a plant enzyme of increasing interest and importan …
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