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Sod1-deficient cells are impaired in formation of the modified nucleosides mcm(5)s(2)U and yW in tRNA.
Xu F, Byström AS, Johansson MJO. Xu F, et al. RNA. 2024 Nov 18;30(12):1586-1595. doi: 10.1261/rna.080181.124. RNA. 2024. PMID: 39322276 Free PMC article.
In addition, the lack of Sod1 induces a defect in the biosynthesis of wybutosine, which is a modified nucleoside found at position 37 of tRNA(Phe) Our results suggest that these tRNA modification defects are caused by superoxide-induced inhibition of the iron-sulfur …
In addition, the lack of Sod1 induces a defect in the biosynthesis of wybutosine, which is a modified nucleoside found at position 37 of …
Ncs2* mediates in vivo virulence of pathogenic yeast through sulphur modification of cytoplasmic transfer RNA.
Alings F, Scharmann K, Eggers C, Böttcher B, Sokołowski M, Shvetsova E, Sharma P, Roth J, Rashiti L, Glatt S, Brunke S, Leidel SA. Alings F, et al. Nucleic Acids Res. 2023 Aug 25;51(15):8133-8149. doi: 10.1093/nar/gkad564. Nucleic Acids Res. 2023. PMID: 37462076 Free PMC article.
Understanding the molecular basis of their virulence is key to develop new treatment strategies. Here, we characterize NCS2*, a point mutation identified in a clinical baker's yeast isolate. Ncs2 is essential for 2-thiolation of tRNA and the NCS2* muta …
Understanding the molecular basis of their virulence is key to develop new treatment strategies. Here, we characterize NCS2*, a point …
Loss of anticodon wobble uridine modifications affects tRNA(Lys) function and protein levels in Saccharomyces cerevisiae.
Klassen R, Grunewald P, Thüring KL, Eichler C, Helm M, Schaffrath R. Klassen R, et al. PLoS One. 2015 Mar 6;10(3):e0119261. doi: 10.1371/journal.pone.0119261. eCollection 2015. PLoS One. 2015. PMID: 25747122 Free PMC article.
In eukaryotes, wobble uridines in the anticodons of tRNA(Lys)UUU, tRNA(Glu)UUC and tRNA(Gln)UUG are modified to 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U). ...Consistent with this notion, we find cellular protein levels drastically decreased in an elp …
In eukaryotes, wobble uridines in the anticodons of tRNA(Lys)UUU, tRNA(Glu)UUC and tRNA(Gln)UUG are modified to 5-metho …
The maize NCS2 abnormal growth mutant has a chimeric nad4-nad7 mitochondrial gene and is associated with reduced complex I function.
Marienfeld JR, Newton KJ. Marienfeld JR, et al. Genetics. 1994 Nov;138(3):855-63. doi: 10.1093/genetics/138.3.855. Genetics. 1994. PMID: 7851780 Free PMC article.
The molecular basis of the maternally inherited, heteroplasmic NCS2 mutant of maize was investigated. Analysis of the NCS2 mtDNA showed that it closely resembles the progenitor cmsT mitochondrial genome, except that the mutant genome contains a fused nad4-nad7 gene …
The molecular basis of the maternally inherited, heteroplasmic NCS2 mutant of maize was investigated. Analysis of the NCS2 mtD …
Lifespan Extension by Retrotransposons under Conditions of Mild Stress Requires Genes Involved in tRNA Modifications and Nucleotide Metabolism.
Maxwell PH, Mahmood M, Villanueva M, Devine K, Avery N. Maxwell PH, et al. Int J Mol Sci. 2024 Oct 1;25(19):10593. doi: 10.3390/ijms251910593. Int J Mol Sci. 2024. PMID: 39408922 Free PMC article.
In this study, we tested a subset of genes identified by RNA-seq to be differentially expressed in S. paradoxus strains with a high-copy number of Ty1 retrotransposons compared with a strain with no retrotransposons and additional candidate genes for their contribution to …
In this study, we tested a subset of genes identified by RNA-seq to be differentially expressed in S. paradoxus strains with a high-c …
Unfolded Protein Response Suppression in Yeast by Loss of tRNA Modifications.
Bruch A, Klassen R, Schaffrath R. Bruch A, et al. Genes (Basel). 2018 Oct 23;9(11):516. doi: 10.3390/genes9110516. Genes (Basel). 2018. PMID: 30360492 Free PMC article.
We demonstrate that two different aggregation prone tRNA modification mutants (elp6 ncs2; elp3 deg1) lacking combinations of 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U: elp3; elp6; ncs2) and pseudouridine (psi: deg1) reduce, rather than increase, splicing …
We demonstrate that two different aggregation prone tRNA modification mutants (elp6 ncs2; elp3 deg1) lacking combinations of 5 …
The tRNA thiolation-mediated translational control is essential for plant immunity.
Zheng X, Chen H, Deng Z, Wu Y, Zhong L, Wu C, Yu X, Chen Q, Yan S. Zheng X, et al. Elife. 2024 Jan 29;13:e93517. doi: 10.7554/eLife.93517. Elife. 2024. PMID: 38284752 Free PMC article.
The evolutionarily conserved thiolation modification of transfer RNA (tRNA) ensures efficient decoding during translation. Here, we show that tRNA thiolation is required for plant immunity in Arabidopsis. ...CGB encodes ROL5, a homolog of yeast NCS6 re …
The evolutionarily conserved thiolation modification of transfer RNA (tRNA) ensures efficient decoding during translati …
tRNA thiolation optimizes appressorium-mediated infection by enhancing codon-specific translation in Magnaporthe oryzae.
Zhang X, He R, Li Y, Ren S, Xiang S, Zheng J, Qu Z, Zhou S, Zhou Z, Chen XL. Zhang X, et al. Nucleic Acids Res. 2025 Jan 7;53(1):gkae1302. doi: 10.1093/nar/gkae1302. Nucleic Acids Res. 2025. PMID: 39777460 Free PMC article.
Thiolation, a post-transcriptional modification catalyzed by Uba4-Urm1-Ncs2/Ncs6 pathway in three specific transfer RNAs (tRNAs), is conserved from yeast to humans and plays an important role in enhancing codon-anticodon interaction and translation efficiency. ...Im …
Thiolation, a post-transcriptional modification catalyzed by Uba4-Urm1-Ncs2/Ncs6 pathway in three specific transfer RNAs (tRNA …
Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling.
Scheidt V, Jüdes A, Bär C, Klassen R, Schaffrath R. Scheidt V, et al. Microb Cell. 2014 Nov 29;1(12):416-424. doi: 10.15698/mic2014.12.179. Microb Cell. 2014. PMID: 28357221 Free PMC article.
To study whether this involves inappropriate TOR signaling, we examined interaction between mutations in TOR pathway genes (tip41 , sap190 , ppm1 , rrd1 ) and U34 modification defects (elp3 , kti12 , urm1 , ncs2 ) and found the rapamycin hypersensitivity in the latter is e …
To study whether this involves inappropriate TOR signaling, we examined interaction between mutations in TOR pathway genes (tip41 , sap190 , …
A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae.
Huang B, Lu J, Byström AS. Huang B, et al. RNA. 2008 Oct;14(10):2183-94. doi: 10.1261/rna.1184108. Epub 2008 Aug 28. RNA. 2008. PMID: 18755837 Free PMC article.
We recently showed that the gamma-subunit of Kluyveromyces lactis killer toxin (gamma-toxin) is a tRNA endonuclease that cleaves tRNA(mcm5s2UUC Glu), tRNA(mcm5s2UUU Lys), and tRNA(mcm5s2UUG Gln) 3' of the wobble nucleoside 5-methoxycarbonylmethyl-2-thi …
We recently showed that the gamma-subunit of Kluyveromyces lactis killer toxin (gamma-toxin) is a tRNA endonuclease that cleaves t …
11 results