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Page 1
Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis.
Sarkar SL, Alam ASMRU, Das PK, Pramanik MHA, Al-Emran HM, Jahid IK, Hossain MA. Sarkar SL, et al. Sci Rep. 2022 Apr 20;12(1):6501. doi: 10.1038/s41598-022-10413-7. Sci Rep. 2022. PMID: 35444203 Free PMC article.
Our customized-designed primers can specifically detect the viral RNA likewise Sansure. We separately optimized SYBR Green RT-PCR reaction of N, E, S, and RdRp genes based on singleplex melting curve analysis at the initial stage. ...Overall, this o …
Our customized-designed primers can specifically detect the viral RNA likewise Sansure. We separately optimized SYBR Green RT- …
A rapid and sensitive one step-SYBR green based semi quantitative real time RT-PCR for the detection of peste des petits ruminants virus in the clinical samples.
Balamurugan V, Sen A, Venkatesan G, Yadav V, Bhanot V, Bhanuprakash V, Singh RK. Balamurugan V, et al. Virol Sin. 2012 Feb;27(1):1-9. doi: 10.1007/s12250-012-3219-z. Epub 2012 Jan 22. Virol Sin. 2012. PMID: 22270801 Free PMC article.
A sensitive and rapid single step real time (rt) RT-PCR was standardized using one-step Brilliant SYBR Green kit for detection and semi-quantitation of peste des petitis ruminants virus (PPRV) using the virus RNA and matrix …
A sensitive and rapid single step real time (rt) RT-PCR was standardized using one-step Brilliant …
Cross-platform evaluation of commercial real-time SYBR green RT-PCR kits for sensitive and rapid detection of European bat Lyssavirus type 1.
Picard-Meyer E, Peytavin de Garam C, Schereffer JL, Marchal C, Robardet E, Cliquet F. Picard-Meyer E, et al. Biomed Res Int. 2015;2015:839518. doi: 10.1155/2015/839518. Epub 2015 Feb 16. Biomed Res Int. 2015. PMID: 25785274 Free PMC article.
This study evaluates the performance of five two-step SYBR Green RT-qPCR kits and five one-step SYBR Green qRT-PCR kits using real-time PCR assays. ...The limit of detection at the 95% confidence level varied between 15 to 9 …
This study evaluates the performance of five two-step SYBR Green RT-qPCR kits and five one-step SYBR …
Increased sensitivity of RT-PCR for Potato virus Y detection using RNA isolated by a procedure with differential centrifugation.
Zhang J, Nie X, Boquel S, Al-Daoud F, Pelletier Y. Zhang J, et al. J Virol Methods. 2015 Dec 1;225:49-54. doi: 10.1016/j.jviromet.2015.07.008. Epub 2015 Jul 22. J Virol Methods. 2015. PMID: 26210699 Review.
The sensitivity of reverse transcription-polymerase chain reaction (RT-PCR) for virus detection is influenced by many factors such as specificity of primers and quality of templates. ...The goal of this study was to enhance the sensitivit …
The sensitivity of reverse transcription-polymerase chain reaction (RT-PCR) for virus detection is …
Real-time reverse transcriptase PCR for the rapid and sensitive detection of Salmonella typhimurium from pork.
Techathuvanan C, Draughon FA, D'Souza DH. Techathuvanan C, et al. J Food Prot. 2010 Mar;73(3):507-14. doi: 10.4315/0362-028x-73.3.507. J Food Prot. 2010. PMID: 20202337
Reverse transcriptase PCR (RT-PCR) detects the presence of mRNA and has a greater potential for detecting viable pathogens than do DNA-based PCR assays, with improved speed and sensitivity compared with traditional methods. ...RNA was ext …
Reverse transcriptase PCR (RT-PCR) detects the presence of mRNA and has a greater potential for detecting …
Real-time reverse-transcriptase--polymerase chain reaction for Salmonella enterica detection from jalapeno and serrano peppers.
Miller ND, Draughon FA, D'Souza DH. Miller ND, et al. Foodborne Pathog Dis. 2010 Apr;7(4):367-73. doi: 10.1089/fpd.2009.0398. Foodborne Pathog Dis. 2010. PMID: 19911882
Outbreaks of Salmonella linked to fresh produce emphasize the need for rapid detection methods to curb the spread of foodborne pathogens. Reverse-transcriptase-polymerase chain reaction (RT-PCR) detects the presence of mRNA (shorte …
Outbreaks of Salmonella linked to fresh produce emphasize the need for rapid detection methods to curb the spread of foodborne pathogens. …
Development and evaluation of a simple and effective RT-qPCR inhibitory assay for detection of the efficacy of compounds towards HIV reverse transcriptase.
Marino-Merlo F, Frezza C, Papaianni E, Valletta E, Mastino A, Macchi B. Marino-Merlo F, et al. Appl Microbiol Biotechnol. 2017 Nov;101(22):8249-8258. doi: 10.1007/s00253-017-8544-6. Epub 2017 Sep 30. Appl Microbiol Biotechnol. 2017. PMID: 28963576
Assessing the actual efficacy of compounds to directly inhibit HIV reverse transcriptase (RT) activity is a main goal in preclinical antiretroviral studies. ...The results obtained with RT-qPCR-IA were compared to that obtained using a one-st …
Assessing the actual efficacy of compounds to directly inhibit HIV reverse transcriptase (RT) activity is a main goal i …
Newly Discovered Natural Hosts of Tomato chlorosis virus in Costa Rica.
Solórzano-Morales A, Barboza N, Hernández E, Mora-Umaña F, Ramírez P, Hammond RW. Solórzano-Morales A, et al. Plant Dis. 2011 Apr;95(4):497. doi: 10.1094/PDIS-11-10-0836. Plant Dis. 2011. PMID: 30743346
Total RNA was extracted from leaf tissue with TRI Reagent (Molecular Research Inc., Cincinnati, OH). Reverse transcription (RT)-PCR reactions were performed with the Qtaq One-Step qRT-PCR SYBR Kit (Clontech Laboratories, Mou …
Total RNA was extracted from leaf tissue with TRI Reagent (Molecular Research Inc., Cincinnati, OH). Reverse transcription (RT …
Development of a quantitative real-time RT-PCR for kinetic analysis of immediate-early transcripts of rat cytomegalovirus.
Loh HS, Mohd-Azmi ML. Loh HS, et al. Acta Virol. 2009;53(4):261-9. doi: 10.4149/av_2009_04_261. Acta Virol. 2009. PMID: 19941390
One-step real-time RT-PCR assay was developed for quantification of the immediate-early (IE), namely IE1 and IE2 transcripts of Rat cytomegalovirus (RCMV), strain ALL-03 in rat embryonic fibroblast cells (REF). This in-house SYBR Green I based …
One-step real-time RT-PCR assay was developed for quantification of the immediate-early (IE), namely IE1 and IE2 …
First Report of Soybean mosaic virus on Soybean in North Dakota.
Nelson BD, Domier LL. Nelson BD, et al. Plant Dis. 2009 Jul;93(7):760. doi: 10.1094/PDIS-93-7-0760C. Plant Dis. 2009. PMID: 30764385
To confirm the presence of SMV, 12 samples that were positive for SMV by DAS-ELISA also were tested by reverse transcription (RT)-PCR. RNA was extracted from sap by a Qiagen RNeasy Plant Mini Kit (Germantown, MD), reverse transcribed, and amplif …
To confirm the presence of SMV, 12 samples that were positive for SMV by DAS-ELISA also were tested by reverse transcription (RT …
11 results