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18,123 results

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Page 1
AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions.
Kido K, Yamanaka S, Nakano S, Motani K, Shinohara S, Nozawa A, Kosako H, Ito S, Sawasaki T. Kido K, et al. Elife. 2020 May 11;9:e54983. doi: 10.7554/eLife.54983. Elife. 2020. PMID: 32391793 Free PMC article.
Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying proteins that interact with a target protein in a cell or organism. ...LC-MS/MS analysis of cells that were stably expressing …
Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and TurboID is a key technology for identifying …
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells.
Roux KJ, Kim DI, Raida M, Burke B. Roux KJ, et al. J Cell Biol. 2012 Mar 19;196(6):801-10. doi: 10.1083/jcb.201112098. Epub 2012 Mar 12. J Cell Biol. 2012. PMID: 22412018 Free PMC article.
We have developed a new technique for proximity-dependent labeling of proteins in eukaryotic cells. Named BioID for proximity-dependent biotin identification, this approach is based on fusion of a promiscuous Escherichia coli biotin protein ligase to a targeting …
We have developed a new technique for proximity-dependent labeling of proteins in eukaryotic cells. Named BioID for proximity-depende …
Off-the-shelf proximity biotinylation using ProtA-TurboID.
Santos-Barriopedro I, van Mierlo G, Vermeulen M. Santos-Barriopedro I, et al. Nat Protoc. 2023 Jan;18(1):36-57. doi: 10.1038/s41596-022-00748-w. Epub 2022 Oct 12. Nat Protoc. 2023. PMID: 36224470 Review.
Proximity biotinylation is a commonly used method to identify the in vivo proximal proteome for proteins of interest. ...Following incubation, during which ProteinA-Turbo antibody-antigen complexes are formed, unbound molecules are washed away, after which bait-prox …
Proximity biotinylation is a commonly used method to identify the in vivo proximal proteome for proteins of interest. ...Follo …
A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues.
Yamanaka S, Horiuchi Y, Matsuoka S, Kido K, Nishino K, Maeno M, Shibata N, Kosako H, Sawasaki T. Yamanaka S, et al. Nat Commun. 2022 Jan 10;13(1):183. doi: 10.1038/s41467-021-27818-z. Nat Commun. 2022. PMID: 35013300 Free PMC article.
Proteolysis-targeting chimaeras (PROTACs) as well as molecular glues such as immunomodulatory drugs (IMiDs) and indisulam are drugs that induce interactions between substrate proteins and an E3 ubiquitin ligases for targeted protein degradation. Here, we develop a w …
Proteolysis-targeting chimaeras (PROTACs) as well as molecular glues such as immunomodulatory drugs (IMiDs) and indisulam are drugs that ind …
BioID: A Screen for Protein-Protein Interactions.
Roux KJ, Kim DI, Burke B, May DG. Roux KJ, et al. Curr Protoc Protein Sci. 2018 Feb 21;91:19.23.1-19.23.15. doi: 10.1002/cpps.51. Curr Protoc Protein Sci. 2018. PMID: 29516480 Free PMC article.
The ligase is fused to a protein of interest and expressed in cells, where it biotinylates proximal endogenous proteins. Because it is a rare protein modification in nature, biotinylation of these endogenous proteins by BioID fusion pr …
The ligase is fused to a protein of interest and expressed in cells, where it biotinylates proximal endogenous proteins …
Identifying Protein-Protein Interactions by Proximity Biotinylation with AirID and splitAirID.
Schaack GA, Sullivan OM, Mehle A. Schaack GA, et al. Curr Protoc. 2023 Mar;3(3):e702. doi: 10.1002/cpz1.702. Curr Protoc. 2023. PMID: 36939277 Free PMC article.
Proximity biotinylation exploits promiscuous biotinylating enzymes fused to a bait protein, resulting in the biotinylation of adjacent endogenous proteins. ...Basic Protocol 1: Construction and functional validation of AirID fusion proteins …
Proximity biotinylation exploits promiscuous biotinylating enzymes fused to a bait protein, resulting in the biotiny …
Protein biotinylation.
Alegria-Schaffer A. Alegria-Schaffer A. Methods Enzymol. 2014;536:109-14. doi: 10.1016/B978-0-12-420070-8.00010-6. Methods Enzymol. 2014. PMID: 24423271
Biotin is a naturally occurring vitamin that binds with high affinity to avidin and streptavidin proteins. Because biotin is small (244 Da), it can be conjugated to many proteins without altering their biological activities. The biotinylated molecule can be d …
Biotin is a naturally occurring vitamin that binds with high affinity to avidin and streptavidin proteins. Because biotin is small (2 …
Biotin protein ligase as you like it: Either extraordinarily specific or promiscuous protein biotinylation.
Cronan JE. Cronan JE. Proteins. 2024 Apr;92(4):435-448. doi: 10.1002/prot.26642. Epub 2023 Nov 23. Proteins. 2024. PMID: 37997490 Free PMC article. Review.
Biotin has biological activity only when covalently attached to a few key metabolic enzyme proteins. Most organisms have only one attachment enzyme, biotin protein ligase (BPL), which attaches biotin to all target proteins. ...For example, the Escherichia col …
Biotin has biological activity only when covalently attached to a few key metabolic enzyme proteins. Most organisms have only one att …
Ubiquitin-specific proximity labeling for the identification of E3 ligase substrates.
Huang HT, Lumpkin RJ, Tsai RW, Su S, Zhao X, Xiong Y, Chen J, Mageed N, Donovan KA, Fischer ES, Sellers WR. Huang HT, et al. Nat Chem Biol. 2024 Sep;20(9):1227-1236. doi: 10.1038/s41589-024-01590-9. Epub 2024 Mar 21. Nat Chem Biol. 2024. PMID: 38514884
Protein ubiquitylation controls diverse processes within eukaryotic cells, including protein degradation, and is often dysregulated in disease. ...Here we report the development of E3-substrate tagging by ubiquitin biotinylation (E-STUB), a ubiquitin-specific …
Protein ubiquitylation controls diverse processes within eukaryotic cells, including protein degradation, and is often dysregu …
Reversible biotinylation of purified proteins for measuring protein-protein interactions.
Dwivedi-Agnihotri H, Srivastava A, Shukla AK. Dwivedi-Agnihotri H, et al. Methods Enzymol. 2020;633:281-294. doi: 10.1016/bs.mie.2019.11.008. Epub 2019 Dec 5. Methods Enzymol. 2020. PMID: 32046851 Free PMC article.
Biotinylated proteins can be directly used as bait for selective immobilization on solid support for measuring protein-protein interactions. Furthermore, biotinylation of protein of interest also allows specific detection in standard bioc …
Biotinylated proteins can be directly used as bait for selective immobilization on solid support for measuring protein- …
18,123 results
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