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9,396 results

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Page 1
Protein refolding using chemical refolding additives.
Yamaguchi S, Yamamoto E, Mannen T, Nagamune T, Nagamune T. Yamaguchi S, et al. Biotechnol J. 2013 Jan;8(1):17-31. doi: 10.1002/biot.201200025. Epub 2012 Sep 11. Biotechnol J. 2013. PMID: 22965925 Review.
The most challenging problem is that the effective condition for refolding is protein dependent and is therefore difficult to select in a rational manner. ...These methods select refolding conditions where productive refolding dominates over unproducti …
The most challenging problem is that the effective condition for refolding is protein dependent and is therefore difficult to …
Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process.
Singh A, Upadhyay V, Upadhyay AK, Singh SM, Panda AK. Singh A, et al. Microb Cell Fact. 2015 Mar 25;14:41. doi: 10.1186/s12934-015-0222-8. Microb Cell Fact. 2015. PMID: 25889252 Free PMC article. Review.
Here we review the developments in the field that are targeted at improving the yield, as well as quality of the recombinant protein by optimizing the individual steps of the process, especially solubilization of the inclusion bodies and refolding of the solubilized …
Here we review the developments in the field that are targeted at improving the yield, as well as quality of the recombinant protein …
Protein refolding for industrial processes.
Clark ED. Clark ED. Curr Opin Biotechnol. 2001 Apr;12(2):202-7. doi: 10.1016/s0958-1669(00)00200-7. Curr Opin Biotechnol. 2001. PMID: 11287238 Review.
Inclusion body refolding processes are poised to play a major role in the production of recombinant proteins. ...
Inclusion body refolding processes are poised to play a major role in the production of recombinant proteins. ...
Non-chromatographic strategies for protein refolding.
Gautam S, Dubey P, Rather GM, Gupta MN. Gautam S, et al. Recent Pat Biotechnol. 2012 Apr;6(1):57-68. doi: 10.2174/187220812799789172. Recent Pat Biotechnol. 2012. PMID: 22420882 Review.
Protein aggregation, a concentration dependent phenomenon, competes with refolding pathway. ...The nanomaterials which disperse well in aqueous buffers are also discussed in the context of facilitating protein refolding. Apart from general results with …
Protein aggregation, a concentration dependent phenomenon, competes with refolding pathway. ...The nanomaterials which dispers …
Smart Protein Refolding System Based on UCST-Type Ureido Polymers.
Tanaka Y, Niu CH, Sasaki T, Nomura S, Maruyama A, Shimada N. Tanaka Y, et al. Biomacromolecules. 2022 Sep 12;23(9):3860-3865. doi: 10.1021/acs.biomac.2c00694. Epub 2022 Aug 28. Biomacromolecules. 2022. PMID: 36030420
In this study, ureido polymers with hydrophobic groups were prepared to evaluate the effects of cooling-induced LLPS of UCST-type polymers on refolding of proteins. When protein was heated with a ureido polymer functionalized with undecyl groups, aggregation of the …
In this study, ureido polymers with hydrophobic groups were prepared to evaluate the effects of cooling-induced LLPS of UCST-type polymers o …
Spatiotemporal Control of Protein Refolding through Flash-Change Reaction Conditions.
Nakahara Y, Kawaguchi T, Matsuda Y, Endo Y, Date M, Takahashi K, Kato K, Okasora T, Ejima D, Nagaki A. Nakahara Y, et al. Langmuir. 2024 Apr 23;40(16):8483-8492. doi: 10.1021/acs.langmuir.4c00024. Epub 2024 Apr 15. Langmuir. 2024. PMID: 38618876
Protein refolding is vital for protein production; however, conventional refolding methods face challenges such as scale-up limitations and difficulties in controlling protein conformational changes on a millisecond scale. ...This FMR system all …
Protein refolding is vital for protein production; however, conventional refolding methods face challenges such …
Online monitoring of protein refolding in inclusion body processing using intrinsic fluorescence.
Igwe CL, Müller DF, Gisperg F, Pauk JN, Kierein M, Elshazly M, Klausser R, Kopp J, Spadiut O, Přáda Brichtová E. Igwe CL, et al. Anal Bioanal Chem. 2024 May;416(12):3019-3032. doi: 10.1007/s00216-024-05249-1. Epub 2024 Apr 4. Anal Bioanal Chem. 2024. PMID: 38573344 Free PMC article.
Industrial process development of protein refolding is a labor-intensive task based largely on empirical approaches rather than knowledge-driven strategies. ...Thus, the methodology presented poses a generic and reliable PAT tool enabling real-time process monitorin …
Industrial process development of protein refolding is a labor-intensive task based largely on empirical approaches rather tha …
In vitro protein refolding by chromatographic procedures.
Li M, Su ZG, Janson JC. Li M, et al. Protein Expr Purif. 2004 Jan;33(1):1-10. doi: 10.1016/j.pep.2003.08.023. Protein Expr Purif. 2004. PMID: 14680955 Review.
In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refo …
In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, …
Polyethylene Glycol-Based Refolding Kinetic Modulation of CRABP I Protein.
Subadini S, Bera K, Behera DP, Hritz J, Sahoo H. Subadini S, et al. Luminescence. 2024 Dec;39(12):e4924. doi: 10.1002/bio.4924. Luminescence. 2024. PMID: 39622558
Crowding environment has a significant impact on the folding and stability of protein in biological systems. In this work, we have used four different sizes of a molecular crowder, polyethylene glycol (PEG), to analyze the unfolding and refolding kinetics of an iLBP …
Crowding environment has a significant impact on the folding and stability of protein in biological systems. In this work, we have us …
Continuous protein refolding and purification by two-stage periodic counter-current chromatography.
Rajendran V, Ponnusamy A, Pushpavanam S, Jayaraman G. Rajendran V, et al. J Chromatogr A. 2023 Apr 26;1695:463938. doi: 10.1016/j.chroma.2023.463938. Epub 2023 Mar 23. J Chromatogr A. 2023. PMID: 37003075
However, the specific activity of the refolded protein (110-130 IU/mg) was less due to the presence of impurities and additives in the refolding buffer. ...High recovery, throughput, and increased operational flexibility make the 2-stage process an attractive option …
However, the specific activity of the refolded protein (110-130 IU/mg) was less due to the presence of impurities and additives in th …
9,396 results