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Refined sgRNA efficacy prediction improves large- and small-scale CRISPR-Cas9 applications.
Labuhn M, Adams FF, Ng M, Knoess S, Schambach A, Charpentier EM, Schwarzer A, Mateo JL, Klusmann JH, Heckl D. Labuhn M, et al. Nucleic Acids Res. 2018 Feb 16;46(3):1375-1385. doi: 10.1093/nar/gkx1268. Nucleic Acids Res. 2018. PMID: 29267886 Free PMC article.
Genome editing with the CRISPR-Cas9 system has enabled unprecedented efficacy for reverse genetics and gene correction approaches. While off-target effects have been successfully tackled, the effort to eliminate variability in sgRNA efficacies-which affect ex …
Genome editing with the CRISPR-Cas9 system has enabled unprecedented efficacy for reverse genetics and gene correction approaches. Wh …
[In silico CRISPR-based sgRNA design].
Wang Y, Chuai G, Yan J, Shi L, Liu Q. Wang Y, et al. Sheng Wu Gong Cheng Xue Bao. 2017 Oct 25;33(10):1744-1756. doi: 10.13345/j.cjb.170182. Sheng Wu Gong Cheng Xue Bao. 2017. PMID: 29082722 Free article. Review. Chinese.
Continuing efforts are made to refine in-silico sgRNA design with high on-target efficacy and reduced off-target effects. In this paper, we summarize the present sgRNA design tools, and show that efficient in-silico models can be built that integrate c …
Continuing efforts are made to refine in-silico sgRNA design with high on-target efficacy and reduced off-target effect …
In Silico Meets In Vivo: Towards Computational CRISPR-Based sgRNA Design.
Chuai GH, Wang QL, Liu Q. Chuai GH, et al. Trends Biotechnol. 2017 Jan;35(1):12-21. doi: 10.1016/j.tibtech.2016.06.008. Epub 2016 Jul 11. Trends Biotechnol. 2017. PMID: 27418421 Review.
CRISPR-based genome editing has been widely implemented in various cell types. In silico single guide RNA (sgRNA) design is a key step for successful gene editing using the CRISPR system, and continuing efforts are aimed at refining in silico sgRNA design wit …
CRISPR-based genome editing has been widely implemented in various cell types. In silico single guide RNA (sgRNA) design is a key ste …