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Year Number of Results
1963 1
1964 1
1968 2
1969 3
1970 1
1971 3
1972 2
1973 5
1974 1
1975 6
1976 4
1977 3
1978 5
1979 4
1980 2
1981 2
1982 3
1983 6
1984 2
1985 8
1986 21
1987 26
1988 87
1989 158
1990 256
1991 378
1992 472
1993 667
1994 929
1995 1149
1996 1226
1997 1444
1998 1542
1999 1759
2000 2024
2001 2131
2002 2259
2003 2369
2004 2634
2005 2714
2006 2917
2007 3003
2008 2903
2009 2893
2010 3162
2011 3531
2012 3635
2013 3858
2014 4010
2015 4241
2016 4221
2017 4815
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2019 6486
2020 7675
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2022 6421
2023 4920
2024 4929
2025 5336
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107,459 results

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Page 1
A quantitative FastFUCCI assay defines cell cycle dynamics at a single-cell level.
Koh SB, Mascalchi P, Rodriguez E, Lin Y, Jodrell DI, Richards FM, Lyons SK. Koh SB, et al. J Cell Sci. 2017 Jan 15;130(2):512-520. doi: 10.1242/jcs.195164. Epub 2016 Nov 25. J Cell Sci. 2017. PMID: 27888217
We coupled the system to an automated imaging workflow capable of handling large datasets. The integrated assay enabled analyses of single-cell readouts at high spatiotemporal resolution. With the assay, we captured in detail the cell cycle alterations induced by an …
We coupled the system to an automated imaging workflow capable of handling large datasets. The integrated assay enabled analyses of s …
Luciferase reporter assay for unlocking ligand-mediated signaling of GPCRs.
Unal H. Unal H. Methods Cell Biol. 2019;149:19-30. doi: 10.1016/bs.mcb.2018.08.001. Epub 2018 Sep 17. Methods Cell Biol. 2019. PMID: 30616819
Genetic reporter assays are based on reporter gene expression in response to activation of specific signaling cascade, followed by monitoring the presence of the reporter protein by directly measuring its enzymatic activity. ...Firefly luciferase gene …
Genetic reporter assays are based on reporter gene expression in response to activation of specific signaling cascade, …
A Novel Reporter Gene Assay for Pyrogen Detection.
He Q, Yu C, Wang L, Ni Y, Zhang H, Du Y, Gao H, Wang J. He Q, et al. Jpn J Infect Dis. 2020 Mar 24;73(2):111-118. doi: 10.7883/yoken.JJID.2019.163. Epub 2019 Oct 31. Jpn J Infect Dis. 2020. PMID: 31666494 Free article.
This study explored the feasibility of a novel reporter gene assay (RGA) for pyrogen detection using RAW264.7 cells stably transfected with the NF-kappaB reporter gene as a pyrogenic marker. ...
This study explored the feasibility of a novel reporter gene assay (RGA) for pyrogen detection using RAW264.7 cells stably tra …
High-Content Reporter Assays.
Cook E, Hermes J, Li J, Tudor M. Cook E, et al. Methods Mol Biol. 2018;1755:179-195. doi: 10.1007/978-1-4939-7724-6_13. Methods Mol Biol. 2018. PMID: 29671271
While luminescent reporter gene assays allow for a rapid and relatively interference free assessment of the activation state of a luminescent reporter, fluorescent reporters do not. ...However, the use of high-content screening allows for the isolation …
While luminescent reporter gene assays allow for a rapid and relatively interference free assessment of the activation state o …
Endogenous Locus Reporter Assays.
Liu Y, Hermes J, Li J, Tudor M. Liu Y, et al. Methods Mol Biol. 2018;1755:163-177. doi: 10.1007/978-1-4939-7724-6_12. Methods Mol Biol. 2018. PMID: 29671270
Traditionally a reporter gene assay is built by cloning an endogenous promoter sequence or synthetic response elements in the regulatory region of a reporter gene to monitor transcriptional activity of a specific biological process (exogenous report
Traditionally a reporter gene assay is built by cloning an endogenous promoter sequence or synthetic response elements in the …
A robust reporter assay for the determination of the bioactivity of IL-4R-targeted therapeutic antibodies.
Yu X, Yu C, Wang K, Liu C, Wang L, Wang J. Yu X, et al. J Pharm Biomed Anal. 2021 May 30;199:114033. doi: 10.1016/j.jpba.2021.114033. Epub 2021 Mar 20. J Pharm Biomed Anal. 2021. PMID: 33774455 Free article.
Bioactivity determination is required to ensure the quality control of mAbs. However, current ELISA and SPR assays or cell-based anti-proliferation assays for IL-4R mAbs are either not mechanism-of-action (MOA) representative or tedious and time consuming. Therefore …
Bioactivity determination is required to ensure the quality control of mAbs. However, current ELISA and SPR assays or cell-based anti …
Development of a novel reporter gene assay for platelet-derived growth factor-BB bioactivity.
Niu A, Zhang Z, Wang X, Wang J, Xu J, Su X, Zhang X, Chen W. Niu A, et al. Biologicals. 2020 Jan;63:68-73. doi: 10.1016/j.biologicals.2019.11.004. Epub 2019 Dec 30. Biologicals. 2020. PMID: 31899075
However, current methods for measuring PDGF bioactivity do not meet the rapidly growing requirements of pharmaceutical research. Here, we describe a novel reporter gene assay (RGA) for PDGF-BB activity measurement. RGA was developed with engineered cells expressing …
However, current methods for measuring PDGF bioactivity do not meet the rapidly growing requirements of pharmaceutical research. Here, we de …
A single secreted luciferase-based gene reporter assay.
Barriscale KA, O'Sullivan SA, McCarthy TV. Barriscale KA, et al. Anal Biochem. 2014 May 15;453:44-9. doi: 10.1016/j.ab.2014.02.019. Epub 2014 Feb 28. Anal Biochem. 2014. PMID: 24583246
Promoter analysis typically employs a reporter gene fused to a test promoter combined with a second reporter fused to a control promoter that is used for normalization purposes. ...We have developed and validated a single secreted luciferase reporter ( …
Promoter analysis typically employs a reporter gene fused to a test promoter combined with a second reporter fused to a …
Affordable luciferase reporter assay for cell-based high-throughput screening.
Siebring-van Olst E, Vermeulen C, de Menezes RX, Howell M, Smit EF, van Beusechem VW. Siebring-van Olst E, et al. J Biomol Screen. 2013 Apr;18(4):453-61. doi: 10.1177/1087057112465184. Epub 2012 Oct 30. J Biomol Screen. 2013. PMID: 23112084
The firefly luciferase gene is commonly used in cell-based reporter assays. Convenient luciferase assay reagents for use in high-throughput screening (HTS) are commercially available. ...The assay was validated in a high-throughput setting using two ca …
The firefly luciferase gene is commonly used in cell-based reporter assays. Convenient luciferase assay reagents for us …
Reporter Gene Assays in Ecotoxicology.
Elad T, Belkin S. Elad T, et al. Adv Biochem Eng Biotechnol. 2017;157:135-157. doi: 10.1007/10_2016_47. Adv Biochem Eng Biotechnol. 2017. PMID: 27928578 Review.
The need for simple and rapid means for evaluating the potential toxic effects of environmental samples has prompted the development of reporter gene assays, based on tester cells (bioreporters) genetically engineered to report on sample toxicity by producing a read …
The need for simple and rapid means for evaluating the potential toxic effects of environmental samples has prompted the development of r
107,459 results
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